Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

Yunita, Rossa; Dewi, Iswari S.; Lestari, Endang Gati; Purnamanengsih, Ragapadmi; Rahayu, Suci; Mastur, -

doi:10.13057/biodiv/d210427

Cited by 5 publications

(6 citation statements)

References 14 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A research by Purnamaningsih et al (2014) states that the regeneration of irradiated callus becomes more restricted than non-irradiated one (control). This is in line with research conducted by Yunita et al (2020), which describes that irradiation dose of 30 Gy causes death in callus above 50% of Batutegi and Situpatenggang variety rice giving. Increased radiation dose of less than 30 Gy callus death more than 70%.…”

Section: Variability Of Relative Fresh Weight Growth Of Handeuleum Ca...supporting

confidence: 91%

“…It is in line with the research of Abdelnour-Esquivel et al (2020) that each ricegenotype generates differing sensitivities for response of several parameters such as survival, regeneration, tissue growth, fresh weight, or dry weight after callus being exposed to gamma rays. Yunita et al (2020) describe that Batugegi dan Situpatenggang rice variety also show different result in sensitivities in every observation of parameters.…”

Section: Callus Relative Fresh Weight Growth (Crfwg) Of Handeuleum Ca...mentioning

confidence: 96%

See 1 more Smart Citation

Effect of Gamma Irradiation on Callus of Handeuleum (Graptophillum pictum L. Griff) Kalimantan and Papua Accession

Rosmala,

Sukma,

Khumaida

2022

J Hort Indonesia

View full text Add to dashboard Cite

Handeuleum (Graptophyllum pictum L.Griff) is a medicinal plant that is widely used as a traditional medicine for its healing properties, hence it should be developed as one of the leading Indonesian medicinal plants. The combination of tissue culture and gamma irradiation are two common methods to improve vegetatively-propagated cultivars. This experiment aims to determine the effect of gamma irradiation dose-rate on the callus culture of the two handeuleum accessions. Callus generated from proliferation were irradiated with gamma rays (0 Gy, 15 Gy, 25 Gy, and 35 Gy) and then grown on regeneration medium containing BAP (4.44 μM and 8.88 μM) and TDZ (4.44 μM and 8.88 μM). The result of the experiment shows that RD50 was at19.25 Gy. The interaction among the Papua accession, gamma irradiation dose-rate of 15 Gy, and regeneration media 4.44 μM BAP produced the highest weight of callus subculture 1 and 2. Irradiation dose-rate of 25 Gy on Kalimantan accession resulted in the highest relative fresh weight growth of irradiated callus. The treatment with a 25 Gy dose-rate produced brownish-white callus and produced the highest value of variability in the relative growth rate of irradiated callus. Keywords: accession, irradiation dose-rate, purple leaves, variability

show abstract

Section: Variability Of Relative Fresh Weight Growth Of Handeuleum Ca...supporting

confidence: 91%

Section: Callus Relative Fresh Weight Growth (Crfwg) Of Handeuleum Ca...mentioning

confidence: 96%

Effect of Gamma Irradiation on Callus of Handeuleum (Graptophillum pictum L. Griff) Kalimantan and Papua Accession

Rosmala,

Sukma,

Khumaida

2022

J Hort Indonesia

View full text Add to dashboard Cite

show abstract

“…Abiotic stress is defined as the detrimental effect of non-living factors on plants in a specific environment including drought, floods, salinity, extreme temperature, heavy metals, and other natural causes. Herwibawa, Sakhidin & Dwi Haryanto (2019) , Amsal & Ishak-Ishak (2018) , Yunita et al (2020) , Meesook, Pongtongkam & Poeaim (2018) , Che Mohd Zain et al (2016) , and Kadhimi et al (2016a) , for example, have conducted research to identify drought-resistant rice cultivars. Researchers from Malaysia, Che Mohd Zain et al (2016) and Kadhimi et al (2016b) exposed rice seeds (MRQ74 and MR269) to 350 Gy of gamma rays and generated osmotic stress using a varied concentration of Polyethylene glycol (PEG) 6,000 ranging from 0–20%.…”

Section: Resultsmentioning

confidence: 99%

“…The ability of the 28H1 and 30F1 rice mutants to absorb water in dry conditions may benefit from having the greatest number of lateral roots. Callus mutation with gamma rays was used by Yunita et al (2020) in upland rice (Situpatengang and Batutegi) where Situpatengang was irradiated with 24.68 Gy gamma rays and Batutegi was with 22.15 Gy gamma rays. Both varieties were tested with 24.11% and 25.18% PEG, respectively, and both mutant calluses were regenerated on MS +BA 3 mg/L + Zeatin 0.1 mg/L, produced 83 shoots Situpatengang and 73 shoots in Batutegi callus.…”

Section: Resultsmentioning

confidence: 99%

A systematic review on physical mutagens in rice breeding in Southeast Asia

Baadu,

Chong,

Gansau

et al. 2023

PeerJ

View full text Add to dashboard Cite

In the 1920s, Lewis Stadler initiated the introduction of permanent improvements to the genetic makeup of irradiated plants. Since then, studies related to breeding mutations have grown, as efforts have been made to expand and improve crop productivity and quality. Stadler’s discovery began with x-rays on corn and barley and later extended to the use of gamma-rays, thermal, and fast neutrons in crops. Radiation has since been shown to be an effective and unique method for increasing the genetic variability of species, including rice. Numerous systematic reviews have been conducted on the impact of physical mutagens on the production and grain quality of rice in Southeast Asia. However, the existing literature still lacks information on the type of radiation used, the rice planting materials used, the dosage of physical mutagens, and the differences in mutated characteristics. Therefore, this article aims to review existing literature on the use of physical mutagens in rice crops in Southeast Asian countries. Guided by the PRISMA Statement review method, 28 primary studies were identified through a systematic review of the Scopus, Science Direct, Emerald Insight, Multidisciplinary Digital Publishing, and MDPI journal databases published between 2016 and 2020. The results show that 96% of the articles used seeds as planting materials, and 80% of the articles focused on gamma-rays as a source of physical mutagens. The optimal dosage of gamma-rays applied was around 100 to 250 Gy to improve plant development, abiotic stress, biochemical properties, and nutritional and industrial quality of rice.

show abstract

“…Therefore, plant biotechnology could help plant breeder by creating and detecting the genetic differences then enhance the yield and quality of tomatoes by increasing the plant tolerance for different stress factors (Abdel-Raheem et al, 2007). In vitro selection to obtain drought tolerant genotypes can use selection agents in the form of osmotic compounds that can simulate dry conditions in the field (Yunita et al, 2020). Polyethylene glycol (PEG) is widely used to induce drought stress under controlled laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Somaclonal Variation Selection for Tolerance to PEG-Induced Drought Stress in Four Local Tomato (Solanum lycopersicum L.) Varieties

Naddaf¹

2020

SJKFU

View full text Add to dashboard Cite

Growth and development of tomato is restricted by numerous abiotic stress factors especially drought. Crop genetic improvement through traditional methods is time-consuming and conducted under uncontrolled conditions; thus, in vitro selection offers an efficient alternative. This research was carried out at the National Commission for Biotechnology (NCBT) and the General Commission for Scientific Agricultural Research (GCSAR), Syria during 2018 and 2019. The main objective was to select callus tolerant to drought stress and regenerate tolerant somaclonal variant plants in four local tomato varieties. Callus (formed from root explants on MS medium supplemented with 2 mg/L BAP and 0.2 mg/L NAA) was subjected to four different levels of drought stress induced by PEG 6000 (2%, 4%, 6%, 8% in addition to the non-treated control). After 8 weeks, cell viability, relative growth rate (RGR), proline content, and tolerance index (TI) were determined. Callus was then transferred to regeneration medium (MS supplemented with 2 mg/L zeatin, 0.2 mg/L GA3 and the sub-lethal concentration of PEG (4% for Daher-Aljabal, 6% for Brieh and 6%, 8% for Daraa variety). The regenerated somaclonal variant plants were evaluated, based on morphological characters, for drought tolerance compared to mother plant. Results showed that RGR, TI and cell viability decreased while free proline content increased in response to increasing PEG concentration. Based on the studied parameters, Daraa was the most tolerant variety, followed by Daher-Aljabal, Brieh and Baskanta. A single regenerated plantlet was obtained from Daher-Aljabal variety named DH1 and another plantlet from Brieh variety named BR1, while two regenerated plantlets were obtained from Daraa variety named DA1 and DA2. All of them showed better tolerance to drought stress than mother plants at 8% PEG concentration in terms of root length, plant height, root-shoot ratio and dry weight. In conclusion, a successful callus formation and plant regeneration protocol has been developed under drought stress treatments induced by PEG 6000. Four somaclonal variant plants were successfully developed and they were different from mother plants based on the studied parameters.

show abstract

Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

Cited by 5 publications

References 14 publications

Effect of Gamma Irradiation on Callus of Handeuleum (Graptophillum pictum L. Griff) Kalimantan and Papua Accession

Effect of Gamma Irradiation on Callus of Handeuleum (Graptophillum pictum L. Griff) Kalimantan and Papua Accession

A systematic review on physical mutagens in rice breeding in Southeast Asia

Somaclonal Variation Selection for Tolerance to PEG-Induced Drought Stress in Four Local Tomato (Solanum lycopersicum L.) Varieties

Contact Info

Product

Resources

About