In vitro production of bovine blastocyst with oocyte collected from abattoir ovary

Deb, Giovanni; Dey, S. R.; Nahar, T. N.; Khan, M. Y. A.; Rahman, Md. Masudur

doi:10.3329/bjas.v45i1.27485

Cited by 7 publications

(10 citation statements)

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“…The lowest percentage of maturation was in M-I stage. This result supported by Vivian et al (2018) who reported the average nuclear maturation in bovine was 58.2%, and lower than Deb et al (2016) who reported 74.16% as detected by extrusion of first polar body and Gabr et al (2015) who reported 67% in TCM -199 medium. However this result was higher than Saleh (2017) who reported 45% by aspiration, 31% in slicing and 32% in aspiration plus slicing.…”

Section: The Effect Of Collection Technique On Ivmsupporting

confidence: 82%

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

et al. 2020

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This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1; the storage duration of ovaries were 6, 9 and 12 h. Experiment -2; the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.

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Section: The Effect Of Collection Technique On Ivmsupporting

confidence: 82%

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

et al. 2020

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“…There is a discrepancy in the reproductive performance between different cattle breeds. Using abattoir-derived ovaries and data base information we studied the effects of breed on in vitro fertilization and early embryo development (4).…”

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confidence: 99%

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Saleh¹

2017

IJVS

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The aim of this study is designed to evaluate the best methods for cow oocytes collection from abattoir specimens which is the cheapest, easily obtained and bulky number. Forty five fresh cow genitalia specimens and testicle were collected directly after slaughter from Al-Shoáalla abattoir northwest of Baghdad the capital early morning, transported in cool box under (4-8) °C to the laboratory of theriogenology in the college of veterinary medicine/Baghdad University during the period from November 2016 to February 2017. Ovaries were separated from the surrounding tissues, washed thoroughly with dis. water repeatedly, then with normal saline and finally with MEM medium containing Antibiotics and Nystatin for contaminant elimination. Oocytes were collected with four methods aspiration, slashing, slicing after aspiration and slicing. The result showed that; the collected oocytes were 55, 68, 87 and 106 oocytes respectively; slicing methods yield more oocytes count. Period of time between slaughtering and samples processing significantly affect oocytes collected percentage and quality, periods as 2, 6, 12 and 24 hours yield 75%, 68%, 61% and 55% oocytes counts of good, fair, poor to aged and bad quality oocytes respectively. Two hours period yield an elevated oocytes count with good quality. Maturation index of oocytes according to the type of collected methods showed 44, 37, 39 and 42 with 12, 8, 6 and 6 good oocyte quality for the four methods respectively. In conclusion slicing methods yield more oocytes count with a moderate quality and embryos production while aspiration methods yield a moderate oocytes count with an elevated quality and good embryos production.

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“…The current oocyte collection followed by their in vitro maturation, in vitro fertilization, in vitro culture and embryo transfer to recipient have improved much to support commercial application of the IVP. Bangladesh Livestock Research Institute (BLRI) has also adopted IVP technology (Deb et al, 2016). The present study reported embryonic development rates to cleavage and to blastocyst stage at BLRI are 75.5±2.7% and 16.6±3.9% respectively.…”

Section: Discussionmentioning

confidence: 63%

“…However, cleavage and blastocyst development rate is little improved than our previous report (Deb et al, 2016). Several factors including oocyte donor, oocytes handling, semen quality, culture media, culture conditions, osmolality of media, CO 2 level of incubator and water quality used for glassware cleaning, media preparation and others steps of IVP influences the rate of blastocyst development (Fukuda et al, 1987, Gordon, 1994, Brum et al, 2005.…”

Section: Discussionmentioning

confidence: 79%

“…Ovaries of slaughtered cows were collected from abattoir located at Mohammodpur, Dhanmondi, Dhaka for IVP embryo production. The IVP protocol was described in previous report (Deb et al, 2016). In brief, 10 cumulus-oocyte-complexes (COC) aspirated from 3 to 8 mm diameter follicles of slaughtered cow ovaries were selected for in vitro maturation (IVM).…”

Section: In Vitro Embryo Production (Ivp)mentioning

confidence: 99%

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Detection of Brucella abortus during in vitro bovine embryo production

Akter¹,

Deb

Miraz

et al. 2021

Bangladesh J. of Livestock Res.

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The in vitro embryo production (IVP) technology has emerged as a potential biotechnological approach to multiply genetically high yielding dairy cows. Its commercial application is increasing in many developed and developing countries over the years. Bangladesh livestock Research Institute (BLRI) adopted in vitro embryo production protocol from bovine ovaries of slaughterhouse. However, the risks of transmission of contagious diseases like Brucella abortus with embryos are not evaluated so far. Considering these facts, the present experiments were conducted to evaluate the efficiency of in vitro embryo production protocol with slaughterhouse ovaries as well as risk of contamination of produced embryos with Brucella abortus. To identify sources of contamination of embryos with Brucella abortus (if any), the laboratory water, different media used in the IVP process, semen, and follicular fluids were evaluated for confirmation of the organisms. In addition, vaginal swabs were collected from 2 buffaloes aborted due to suspected Brucella abortus infection. Molecular test were used to detect Brucella abortus contamination. Brucella abortus specific PCR product was not detected on agarose gel electrophoresis. The efficiency of IVP measured by cleavage and blastocyst development rates were 75.5±2.7% and 16.6±3.9%, respectively. The present study inferred that the in vitro produce embryos are free from Brucella abortus infection. Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 105-112

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In vitro production of bovine blastocyst with oocyte collected from abattoir ovary

Cited by 7 publications

References 0 publications

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Detection of Brucella abortus during in vitro bovine embryo production

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