The aim of this study is designed to evaluate the best methods for cow oocytes collection from abattoir specimens which is the cheapest, easily obtained and bulky number. Forty five fresh cow genitalia specimens and testicle were collected directly after slaughter from Al-Shoáalla abattoir northwest of Baghdad the capital early morning, transported in cool box under (4-8) °C to the laboratory of theriogenology in the college of veterinary medicine/Baghdad University during the period from November 2016 to February 2017. Ovaries were separated from the surrounding tissues, washed thoroughly with dis. water repeatedly, then with normal saline and finally with MEM medium containing Antibiotics and Nystatin for contaminant elimination. Oocytes were collected with four methods aspiration, slashing, slicing after aspiration and slicing. The result showed that; the collected oocytes were 55, 68, 87 and 106 oocytes respectively; slicing methods yield more oocytes count. Period of time between slaughtering and samples processing significantly affect oocytes collected percentage and quality, periods as 2, 6, 12 and 24 hours yield 75%, 68%, 61% and 55% oocytes counts of good, fair, poor to aged and bad quality oocytes respectively. Two hours period yield an elevated oocytes count with good quality. Maturation index of oocytes according to the type of collected methods showed 44, 37, 39 and 42 with 12, 8, 6 and 6 good oocyte quality for the four methods respectively. In conclusion slicing methods yield more oocytes count with a moderate quality and embryos production while aspiration methods yield a moderate oocytes count with an elevated quality and good embryos production.

Thirty six trials of flushing with riched Delbeco`s phosphate buffer media applied in this study using a plastic two ways folly catheter, introduced by metal robe inside the cervix of donor cows and by air bubble fixed it in the cervical canal in 3 positions, one near the cervico-vaginal opening or at the first third of the cervical canal, the 2nd one at the middle third of the cervical canal and the 3rd one near the utero-cervical opening or at the last third of the cervical canal. The process of flushing was carried out after the fixation of the folly catheter in the three sites and the number of embryos collected by each collection site.The results showed that the number of embryos detected in the third method where the fixation was in the last 3rd of cervix relatively more than the other two sites.The study showed that the position and the fixation of the folly catheter in the last 3rd of the cervical canal and toward the utero-cervical opening gave more number of embryos than the two other sites upon the process of flushing and collection of embryos.

     Sperm capacitation refers to the physiological changes in which spermatozoa must undergo in order to have its ability to penetrate and fertilize a mature oocyte. Recognition of the phenomenon was quite important to early in vitro fertilization (IVF) experiments as well as to the fields of embryology and reproductive biology. This initial study is designed to study the process of capacitation of ram sperm with three heparin levels (50,100,150 IU). 140 pairs of abattoir testicular samples (70 pairs each season), with 70 ewe genitalia specimens (35 each season). Spermatozoa sample were capacitated with heparin in CO2 incubator for 45 minutes, and evaluated by its total motility and its ability to penetrate oocytes zona pellucida for fertilization. Different heparin level for spermatozoa capacitation in relation to seasonal influences revealed that; in regarding to capacitation index there was an increased spermatozoa activity and motility significantly for the three levels within season, but capacitation index showed significant variations between the three heparin levels out of breeding season in which the highest heparin level gave the best capacitation index than the other two. Results of oocytes collection in relation to seasonal influences revealed to; total oocytes number was 2035 oocytes on both season in which 1039 oocytes were obtained within season while a number of 1006 oocytes was collected out of season. Oocytes maturity index within season time is about 40% for the oocytes grade V.good and good grade, fair and poor grade is 25%.Oocytes collected out of breeding time showed range between 25% for the v. good and good grade to 10% for the other. Fertilizable index for embryos development after capacitated spermatozoa adding to the medium containing matured oocytes is range from 35% for the v. good and good graded oocytes within a season to 10% out of season. In conclusion; heparin can induced spermatozoa capacitation in any levels through the breeding season while the high level of heparin gave better results, fertilizable index and embryo production are both gave best result via breeding season.