Detection of Brucella abortus during in vitro bovine embryo production

Akter, Arzuba; Deb, Giovanni; Miraz, Mfh; Kabir, MA; Hossain, Smj; Islam, Soriful; Sk, Dey

doi:10.3329/bjlr.v27i1.55174

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…2). The cleavage and blastocyst rate of slaughterhouse driven COCs in conventional culture dish was found to be 75.5±2.7% and 16.6±3.9% which are in accordance with our current GPS and drop culture cleavage and blastocyst rate [14]. Different plastic dishes with varying sizes and shapes are used to culture gametes and COCs.…”

Section: Resultssupporting

confidence: 86%

Development of Bovine Embryo Culture System for Maintaining Genetic Identity of Embryos

Deb,

Miraz,

Hossain

2024

AJRAVS

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Cumulus-oocyte complexes (COCs) recovered during an ovum pick-up (OPU) session from a cow is low (1 to 15 COCs). Moreover, mixing of two or more cows COCs to increase total number in a culture droplet will loss the genetic identity of embryos. The present study was aimed to develop a culture system suitable for culture of smaller number of COCs (less than 10 COCs). Slaughterhouse bovine ovaries were used for the experiment. Aspirated oocytes possessing an even cytoplasm and covered with minimum 3 layers of compact cumulus cell were subjected to in vitro maturation (IVM) followed by in vitro fertilization (IVF) using fresh semen capacitated by treating with heparin sodium salt. Cumulus cells were removed by gentle pipetting and denuded zygotes were subjected to in vitro culture (IVC) for blastocyst development. Cleavage rates were evaluated at day 3 (day 0: day of IVF) and blastocyst rate at day 8 as a proportion of the presumed zygote transferred into IVC-I medium. Results show that development rate up to cleavage did not affect by COCs densities. Single COCs did not reach to the blastocyst stage when cultured individually. The development rate to the blastocyst stage was 13.9±9.0%, 10.8±4.9% and 16.6±3.9% for 3, 5, and 10 COCs groups,

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Section: Resultssupporting

confidence: 86%

Development of Bovine Embryo Culture System for Maintaining Genetic Identity of Embryos

Deb,

Miraz,

Hossain

2024

AJRAVS

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“…Research in the field of embryo culture and transfer remains notably limited in Bangladesh. So far, progress has been achieved mainly in optimizing culture conditions and research for enhancing blastocyst development [3]- [5]. Bangladesh has Deb et al…”

Section: Introductionmentioning

confidence: 99%

Birth of First In vitro Produced Calves in Bangladesh and Their Reproductive Fitness

Deb,

Miraz,

Kabir

et al. 2024

EJVETMED

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The goal of the current study is to produce calves by implanting recipient cows with in vitro generated embryos. For in vitro maturation, slaughterhouse-driven cumulus-oocyte-complexes (COCs) with an even cytoplasm and at least three layers of compact cumulus cells were chosen (IVM). For the purpose of in vitro fertilization, capacitated fresh spermatozoa and presumed matured COCs were co-cultured for 18 to 20 hours (IVF). Cumulus cells were removed by carefully pipetting them into TL-HEPES. The zygotes spent three days in an in vitro culture (IVC) droplet. After this initial phase of culture, the 8–32 cell embryos were moved to IVC-II media, where they remained until Day 8 of development. On day 8, these embryos were transferred into recipient cows, with two embryos transferred to each of the five recipient cows. On day 60 of embryo transfer (ET), rectal palpation was used to assess pregnancy followed by ultrasonography on day 90. Out of five recipients, one cow delivered twine calves with a pregnancy rate of 20%. The calves were named Falguni and Chaitali. The duration of gestation was 277 days. Falguni weighed 12.87 kg at birth, whereas Chaitali weighed 18.59 kg. Falguni and Chaitali both showed typical growth patterns, with an average 254.77 g and 463.15 g/day. At a regular age, both heifer calves reach puberty and give birth. This research emphasizes the possibility that ovocytes from slaughterhouse ovaries can be used to produce healthy IVP calves, which would be a major step forward in Bangladesh’s fast expansion of high-yield dairy cattle.

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“…There are numerous irrefutable evidences of colonization of the uterus in animals infected by Brucella spp. but there is, so far, no evidence of the presence of the bacterium in the ovary of infected animals (Akter et al., 2020; Neglia et al., 2007).…”

Section: Introductionmentioning

confidence: 99%

Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in vitro embryos?

Andrade,

Custódio,

Silva Fernandes do Vale

et al. 2024

Reprod Domestic Animals

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Animal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect Brucella spp. in the ovarian follicular fluid of brucellosis‐positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from Brucella‐positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus Brucella (bcsp31 gene). All 47 bovine aspirates were negative for Brucella spp. 0.00% (95% CI: 0.00–4.00%). Our results demonstrated that Brucella spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that Brucella spp.‐infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis‐seropositive cows to transfer embryos to heifers/cows not infected by Brucella, aiming to produce calves free of the infection.

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Detection of Brucella abortus during in vitro bovine embryo production

Cited by 3 publications

References 11 publications

Development of Bovine Embryo Culture System for Maintaining Genetic Identity of Embryos

Development of Bovine Embryo Culture System for Maintaining Genetic Identity of Embryos

Birth of First In vitro Produced Calves in Bangladesh and Their Reproductive Fitness

Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in vitro embryos?

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