In Vitro Pathogenicity of Some Entomopathogenic Fungal Strains against Green Peach Aphid Myzus persicae (Homoptera: Aphididae)

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Pathogenicity of four fungal strains, two of Beauveria bassiana (designated as BB-72 and BB-252), and two of Lecanicillium lecanii (designated as V-4 and CS-625) to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae) was assessed. All treatments showed highly significant effects on the mortality of the aphid when applied as filtrate or conidia. The application methods did not have a significant effect on the mortality rate. The overall mortalities caused by both applications were statistically similar. The maximum overall mortality was caused by BB-72, followed by CS-625 in both application methods. On the other hand, (V-4) caused the minimum mortality rate, followed by (BB-252), which was statistically similar to those caused by V-4. The time also had significant effects on aphid mortality rates. The mortality was the minimum after 3 days reaching its maximum after 10 days. As the number of days increased, there was a corresponding increase in the mortality showing a direct relationship between mortality and time. The maximum mean individual mortality of 95% was caused by CS-625, followed by BB-72 (92%) after 10 days with the conidial application. Similarly, the combined effects of the three most virulent fungal strains showed highly significant differences on the mortality of peach aphid. The combination BB-72 + BB-252 showed the highest percent mortality, followed by BB-72 + BB-252 + CS-625. The combinations BB-72 + CS-625 and BB-252 + CS-625 had statistically similar effects of causing aphid mortality.

Section: Resultssupporting

confidence: 56%

Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae)

Javed

Mukhtar

et al. 2019

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“…Earlier studies have been conducted with different species or isolates of EPF against different host species, which showed different pathogenicity. For instance, as a result of the application of B. bassiana (BB-72 and BB-252) and L. lecanii (V-4) isolates to Myzus persicae individuals, 95, 91, and 87% mortality rates were recorded (Nazir et al 2018). In another study, B. bassiana had been reported to have an effect over 75%, as a result of application of BAU004, BAU018, and BAU019 isolates to the same aphid species (Al-alawi and Obeidat 2014).…”

Section: Resultsmentioning

confidence: 99%

Effects of some entomopathogenic fungi on the aphid species, Aphis gossypii Glover (Hemiptera: Aphididae)

Erol

Abdelaziz

Birgücü

et al. 2020

Background Effects of the entomopathogenic fungi Beauveria bassiana, Verticillium alfalfae, and Trichoderma viride; secondary metabolites of MS1 (B. bassiana) and MS2 (V. alfalfae); and Dimethoate active substances on the aphid species, Aphis gossypii Glover (Hemiptera: Aphididae), were tested. Main body of the abstract Fungus isolates were prepared as 107 conidia ml−1 of spore suspensions and applied on the 2nd instar nymphs of A. gossypii. After the applications, evaluations were made on the 1st, 3rd, 5th, and 7th days by counting the live individuals. Obtained data were 100 and 93% mortality rate at MS1 (B. bassiana) and MS2 (V. alfalfae), and secondary metabolites were recorded in the 3rd day count results. On the 5th day counts, the highest mortality rates after secondary metabolites were statistically at the same group with B. bassiana, T. viride, and dimethoate. On the 7th day, counting results of all experiment groups were analyzed statistically and were found effective. Short conclusion Obtained results showed that the fungal secondary metabolites might be useful when utilized as a biocontrol agent against the aphids.

“…Ortiz et al (2016) reported LC 50 of 1.07 × 10 7 to 1.31 × 10 10 condia ml −1 of different (Kumar and Kaur 2017). Nazir et al (2019) tested isolates of B. bassiana (BB-72 and BB-252) against green peach aphid, Myzus persicae, where they recorded high mortality of aphids (95 and 91%, respectively) after 10 days of treatment. Different isolates from different countries have different potential in the management of different insect pests.…”

Section: Efficacy Against Spodoptera Lituramentioning

confidence: 99%

Molecular characterization of new isolates of the entomopathogenic fungus Beauveria bassiana and their efficacy against the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Dhar

Jindal

Jariyal

et al. 2019

Thirteen fungal isolates, having characteristics similar to Beauveria bassiana, were isolated from field soils of Punjab, India, on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolates, using PCR amplification with B. bassiana-specific primer and nucleotide sequence analysis of ITS region, confirmed only three isolates as B. bassiana (now named as BbR1, BbR2, and BbR3) along with MTCC 2028 reference isolate. Comparative RAPD-PCR amplification with ten different RAPD primers showed that all the three isolates were closely related. Genetic relatedness dendrogram developed, using RAPD-PCR data by UPGMA, measured the quantitative description of genetic diversity and showed that the standard isolate MTCC2028 was 58% similar to new isolates. B. bassiana isolates achieved their maximum growth during the optimum incubation period of 7 days. Significant mortality rates of the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), were recorded at all the tested concentrations of conidial suspensions (10 3 to 10 7 conidia ml −1 ) of B. bassiana. BbR2 was found to be the most potential local isolate, causing the maximum mortality (83.33%) of S. litura, with LC 50 value of 935.663 conidia ml −1 , followed by BbR3 (74. 33%), BbR1 (72.22%), and BbM1 (67.12%) isolates.