In vitro organogenesis of Passiflora alata

Abstract

Passiflora alata in vitro organogenesis was studied based on explant type, culture medium composition, and incubation conditions. The results indicated that the morphogenic process occurred more efficiently when hypocotyl segment-derived explants were cultured in media supplemented with cytokinin and AgNO 3 incubated under a 16-h photoperiod. The shoot bud elongation and plant development were obtained by transferring the material to MSM culture medium supplemented with GA 3 and incubated in flasks with vented lids. Histological analyses of the process revealed that the difficulties in obtaining plants could be related to the development of protuberances and leaf primordia structures, which did not contain shoot apical meristem. Roots developed easily by transferring elongated shoots to 1/2 MSM culture medium. Plant acclimatization occurred successfully, and somaclonal variation was not visually detected. The efficiency of this organogenesis protocol will be evaluated for genetic transformation of this species to obtain transgenic plants expressing genes that can influence the resistance to Cowpea aphid borne mosaic virus.

Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)

“…1). Adventitious shoot production from leaf segment has been previously observed in P. alata (Pinto et al 2010;Rodriguez et al 2007). The use of cytokinins for in vitro organogenesis in Passiflora species has also been reported with induction of adventitious buds in response to BAP alone or in association with NAA, TDZ or kinetin (Becerra et al 2004;Dornelas and Vieira 1994;Hall et al 2000;Trevisan and Mendes 2005).…”

“…Shoots from root explants of P. cincinnata and P. edulis grown in MS medium with 2.89 uM GA 3 were elongated about 2 cm after 10 days in culture . The shoot bud elongation of P. alata was more efficient using 2.88 uM GA3, its percentage was 35.0%, on the other hand, none elongated shoots were obtained in control medium (Pinto et al 2010). Table 3 showed the effect of IAA on in vitro rooting of shoots developed from leaf explants of purple passion fruit after 14 weeks of culture.…”

“…Pinto et al (2010) reported that in vitro organogenesis induction of sweet passion fruit occurred more efficiently when hypocotyl segment-derived explants were cultured in MS medium supplemented with BAP and AgNO 3 under 16-h photoperiod. Trevisan et al (2005) also reported that the bud induction and shoot development of P. edulis Sims.…”

“…Several studies also reported that the plant regeneration of passion fruit could be obtained via organogenesis from a wide range of species and types of explants such as leaf, hypocotyl, root and cotyledon (Braglia et al 2010;Fernando et al 2007;Lombardi et al 2007;Nhut et al 2007;Pinto et al 2010;Silva et al 2011). Two morphogenic pathways are known to be related to in vitro plant differentiation and regeneration process, de novo organogenesis and somatic embryogenesis (Rochas et al 2015).…”

Concentrations of Silver Nitrate in the In Vitro Development and Conservation of <i>Passiflora gibertii</i> N. E. Brown

“…It was observed that direct organogenesis occurred more efficiently when explants of P. alata were cultured in media supplemented with silver nitrate and cytokinins, recommended the supplementation for the formation of plants buds [34].…”