In vitro neoplastic transformation of newborn hamster salivary-gland tissue by oncogenic DNA viruses

Abstract

Explant cultures of newborn hamster salivary-glands were infected with 4 oncogenic DNA viruses: polyoma virus, human adenovirus 12, simian virus 40 (SV40) and the LLE46 strain of adenovirus 7 (the adenovirus 7-SV40 "hybrid").Only SV40 and LLE46 infected tissues underwent "transformation." Subcutaneous injection of SV40 transformed cells into irradiated adult hamsters produced fibrosarcomas. Subcutaneous injection of LLE46 transformed cells, however, produced tumors which were bimorphic with 2 distinct histological elements, one composed of small hyperchromatic undifferentiated cells resembling a n adenovirus type tumor a n d the other resembling a n SV40-induced sarcoma. No evidence of salivary-gland function as indicated by amylase production could be demonstrated in the transformed cultures or i n the tumors which they produced. N 1959 DAWE AND L A W~ OBSERVED THAT NEW-I born mouse salivary-glands, infected with polyoma virus and grown as organ cultures in vitro, developed morphologic characteristics of neoplasia. Vogt and Dulbeccolfi reported transformation of cellular morphology and growth characteristics of monolayer cultures of mouse and hamster embryo cells exposed in vitro to polyoma virus. They also were able to induce progressively growing neoplasms in adult hamsters by subcutaneously transplanting the transformed hamster embryo cells.Subsequently simian virus 40 (SV40), anFrom the Surgery and Pathologic Anatomy Branches,

Viral Neoplastic Transformation of Hamster Prostate Tissue in vitro

“…The transformation mnaxb,e spontaneouIS (1), chemnica,lly indUced (2, 3), or pro-dlUced by infection wvith a varietv of oncoglenic ViruLses (4,5). Transformnation in V itro Of nliurine prostatic tISSuies bx miethv,lcholanthrene waI,s recently, reported: the transformed cells pro-CILuCedI a xvarietx of histo logica,l types of tumor01S wvhen imiplantecd in svIngeneic hosts (3).…”

“…lateral aiidc v entral-lobe portions. It was niiincedi into 1 -mmii explants land theii uised to establish txxo 2-ountce (57 g) presc-riptioii bo)ttle CUltures fromi each lobe bx miethodis described (5). Wheni the cells began to migrate fr-omi the explants on the 2thd aind 3rd diaxvs, one Cuilture-bOttle eachi of dorsal-, laterald-.…”

In Vitro Transformation by Adenovirus-Simian Virus 40 Hybrid Viruses

“…This tumor probably arose from transformation with the transcapsidant particle and presumably contains the ad 7 and SV40 genomes but no ad 12 genetic information. In the majority of studies, mixed or dimorphic tumors resulted from inoculation of animals with transcapsidant viruses (22,26,35,36). Such tumors probably represent several oncogenic events by the various particles in the hybrid population and are analogous to some of the mass culture lines in the present and past studies (11).…”