In Vitro Transformation by Adenovirus-Simian Virus 40 Hybrid Viruses

Abstract

Clones were isolated from hamster cells transformed by the adenovirus 2-SV40 and adenovirus 12-SV40 transcapsidant hybrid viruses. The clones were characterized with respect to their cytomorphology, virus and antigen content, and the histomorphology of tumors induced by transplantation of the clonal sublines to hamsters. Three different cellular and colonial morphologies were observed. Clones with an SV40 morphology gave rise to tumors predominantly with an SV40 histology, whereas clones with an adenovirus morphology produced typical adenovirus tumors upon transplantation of the transformed cells. Clones which had features of both SV40 and adenovirus transformed cells gave rise to “intermediate” and adenovirus tumors. The results indicate that multiple events occur during transformation and tumorigenesis by the transcapsidant virus populations and provide an explanation for the multiplicity of findings which have been reported with these virus populations.

Contact-Inhibited Revertant Cell Lines Isolated From Sv40-Transformed Cells

“…The presence of the S V40-specific T antigen was determined by the indirect fluorescent antibody technique as described (10) . SV40 virus was rescued from SV-3T3 and revertant cells by fusion with MA-134 cells, a continuous cell line derived from African green monkey kidney cells (AGMK), in the presence of Sendai fusion factor, as described by Burns and Black (11) .…”