Viral Neoplastic Transformation of Hamster Prostate Tissue in vitro

Paulson, David F.; Rabson, Alan S.; Fraley, Elwin E.

doi:10.1126/science.159.3811.200

Cited by 34 publications

(7 citation statements)

References 6 publications

(2 reference statements)

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“…These observations contrast with previous studies reporting morphological transformation of epithelial cells in vitro (Black & Rowe, 1963;Paulson et al 1967). The contrasting results may stem from the fact that the epithelial cells used by other investigators were derived from different species, or from different organs, than the lines described in this paper.…”

Section: Simian Virus 40 and Moloney-murine Sarcoma Virus Infection Ofcontrasting

confidence: 56%

Simian Virus 40 and Moloney-Murine Sarcoma Virus Infection of Bona fide Mouse Epithelium

Anderson

Smith

1980

Journal of General Virology

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Section: Simian Virus 40 and Moloney-murine Sarcoma Virus Infection Ofcontrasting

confidence: 56%

Simian Virus 40 and Moloney-Murine Sarcoma Virus Infection of Bona fide Mouse Epithelium

Anderson

Smith

1980

Journal of General Virology

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“…Additional evidence supporting a venereally transmitted agent, in the etiology of the disease comes from animal studies, in which prostate carcinoma has been induced in vitro by the SV40 oncogenic virus (Paulson et al, 1968). Findings of virus-like particles in human prostatic cancers (Tannenbaum, 1968) and the increased incidence of cervical carcinoma reported in spouises of prostatic cancer patients (Feminella & Lattimer, 1974) lend more support to this hypothesis.…”

Section: Resultsmentioning

confidence: 99%

Descriptive epidemiology of testicular and prostatic cancer in Los Angeles

Ross¹,

McCurtis²,

Be³

et al. 1979

Br J Cancer

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Summary.-Data from the Los Angeles County Cancer Surveillance Program (CSP) from 1972 to 1975 were used to study the descriptive epidemiology of testicular cancer and prostatic cancer. The very high black/white ratio and late age peak of cancer of the prostate contrasted sharply with the very low ratio and early age peak of testicular cancer. However, both sites had higher rates among upper occupational and social class groupings. Available descriptive and analytical research suggests that the etiology of prostatic cancer is most probably related to hormonal influences rather than to a horizontally transmitted agent, while the etiology of testicular cancer is most probably related to endogenous or exogenous hormonal influences in utero or in infancy, or to in utero exposure to other exogenous agents.

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“…Other possible reasons include the selection of the cell line from a precursor cell rather than a plasma cell, the absence of antigen in the culture, or the failure to secrete antibody, which may inhibit production. Furthermore, transformation of a differentiated cell with SV40 frequently leads to a decrease in specialized function (21)(22)(23)(24)(25)(26).…”

Section: Trsc-1 Cells Grown In the Presence Of [4c]ileucine Ormentioning

confidence: 99%

Transformation by Simian Virus 40 of Spleen Cells from a Hyperimmune Rabbit: Evidence for Synthesis of Immunoglobulin by the Transformed Cells

Collins¹,

Black²,

Strosberg³

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

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Spleen cells derived from a rabbit hyperimmunized with a Type III pneumococcal vaccine were exposed to simian virus 40 in vitro. After 114 days, transformed cells growing on the surface of one culture dish were observed and a cell line was established. The transformed cells had a morphology characteristic of cells transformed by simian virus 40, contained the simian virus 40-specific T antigen, and yielded infectious simian virus 40 upon cultivation with indicator cells in the presence of Sendai fusion factor. Transformed cells incorporated labeled amino acid into protein with the antigenic properties of rabbit immunoglobulin G.We have previously attempted to obtain a virus-transformed cell line capable of indefinite growth in vitro and continuous production of specific antibody (1). Lymph-node cells from immunized rats were exposed to simian virus 40 (SV40); four transformed cell lines were obtained, but none was found to synthesize immunoglobulin or immunoglobulin fragments. Attempts to establish a relationship between the transformed cells and lymphoid cells were inconclusive, and the identity of the target cell for transformation remained uncertain. In order to increase the likelihood of successful transformation of antibody-producing cells, resort was made, in the present experiments, to cells from a rabbit hyperimmuniized with a pneumococcal vaccine. Rabbits injected intravenously with pneumococcal vaccines produce large amounts of antibody of restricted heterogeneity (2). Furthermore, marked plasmacell infiltration of the spleen has been demonstrated (3). This report describes the infection with SV40 of spleen cells from one such rabbit, and the subsequent establishment of a transformed cell line capable of producing rabbit immunoglobulin of the IgG class. In the accompanying manuscript, the presence of a protein of markedly restricted heterogeneity that specifically binds the immunizing antigen is demonstrated (4). MATERIALS AND METHODSImmunization. Rabbit L-27 was kindly provided by Drs 6). When the rabbit was killed, the serum contained 11 mg of antibody against S3 pneumococcal polysaccharide per ml. Isoelectric focusing of purified antibody showed three major and one minor species (4).Initiation and Maintenance of Stationary Suspension Cultures. These procedures were performed essentially as described for rat lymph-node cells (1). Briefly, rabbit L-27 was killed. The spleen was removed, minced finely, and pressed through wire mesh. Cells were suspended in medium RPMI 1640, supplemented with 20% unheated fetal-calf serum, 2 mM glutamine and antibiotics, infected with virus (see below), seeded in 60-mm diameter plastic dishes, and maintained in the above medium at 370 in a humidified atmosphere of 5% CO2 in air. Half of the medium of each plate was changed weekly, and 3 days after each medium change each plate was supplemented with '/lo volume of fresh medium. Bonemarrow cells were obtained by aspiration, dispersed by repeated pipetting, and treated as above.Virus Transformation of Suspension Cu...

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Viral Neoplastic Transformation of Hamster Prostate Tissue in vitro

Cited by 34 publications

References 6 publications

Simian Virus 40 and Moloney-Murine Sarcoma Virus Infection of Bona fide Mouse Epithelium

Simian Virus 40 and Moloney-Murine Sarcoma Virus Infection of Bona fide Mouse Epithelium

Descriptive epidemiology of testicular and prostatic cancer in Los Angeles

Transformation by Simian Virus 40 of Spleen Cells from a Hyperimmune Rabbit: Evidence for Synthesis of Immunoglobulin by the Transformed Cells

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