In Vitro Model for Modulation of Helper T Cell Differentiation and Activation

Heo, Yong

doi:10.1002/0471140856.tx1809s24

Cited by 5 publications

(6 citation statements)

References 22 publications

(31 reference statements)

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“…This study showed that the proliferation of T-cell stimulation by con A was increased by the induced AD and decreased by the administration of GTT. The type-2 response becomes predominant when the differentiation or activation of Th2 cells is preferred, but development or stimulation of Th1 cells is suppressed [12, 27]. Orally administered GTT decreased the IgE and increased the IgG2a in the blood of NC/Nga mice.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effects of green tea extract from tannase digests on house dust mite antigen-induced atopic dermatitis-like lesions in NC/Nga mice

Hwang

Chang

Kim³

et al. 2019

Arch Dermatol Res

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Atopic dermatitis (AD) is one of the most common chronic inflammatory skin diseases, which is affected by several factors. Anti-histamines, steroids, and immunosuppressive agents have been used for the treatment of AD. However, many studies have reported that long-term use and abuse of these drugs causes many side effects. This study was performed to evaluate the ameliorative effect of green tea extract on AD-like lesions in NC/Nga mice. Green tea extract from tannase digest (GTT), beta-hexosaminidase, and histamine were measured in IgE-antigen complex-stimulated RBL-2H3 cells. Dorsal skin application of house dust mite-ointment induced AD-like symptoms in NC/Nga mice. Dermatitis scores, skin moisture, transepidermal waterloss (TEWL), thickness of skin and ear, T-cell proliferation, levels of immunoglobulins and cytokines, and infiltration of mast cell were measured to assess the degree of AD induction. Skin moisture and TEWL were measured using probes, and ELISA was performed to measure the immunoglobulin and cytokine levels in blood. GTT was selected based on its ability to inhibit the release of beta-hexosaminidase and histamine in IgE-antigen complex-stimulated RBL-2H3 cells. Oral administration of GTT significantly suppressed the skin inflammation and symptoms of AD-like skin lesions in NC/Nga mice. GTT may have a potential therapeutic effect in the treatment of AD.

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Section: Discussionmentioning

confidence: 99%

Ameliorative effects of green tea extract from tannase digests on house dust mite antigen-induced atopic dermatitis-like lesions in NC/Nga mice

Hwang

Chang

Kim³

et al. 2019

Arch Dermatol Res

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“…The most important aspect to be discussed is GC oil's systemic effect on the modulation of the in vivo type-2 response. The type-2 response becomes predominant when differentiation or activation of Th2 cells is preferred, but development or stimulation of Th1 cells is suppressed [ 13 , 14 ]. Considering that the skewedness toward type-2 responses is a background mechanism for the occurrence of atopic dermatitis [ 1 , 4 , 25 ], dermal application of GC oil could systematically block the pathogenesis of atopic dermatitis through correcting the immune homeostasis skewed in favor of Th2.…”

Section: Discussionmentioning

confidence: 99%

“…Interleukin-4 (IL-4) production from Th2 cells, mast cells, or other immune components cells is known to trigger isotype-switching to IgE and IgG1 in B cells [ 18 ]. Evaluation of IL-4 production from splenic T cells is a tool for investigating the predominance of Th2 reactivity [ 13 ]. Hyper IgE production is a hallmark of atopic dermatitis in humans [ 1 , 4 ], and well documented in transgenic mice or NC/Nga mice suffering from atopic dermatitis-like skin lesions [ 8 , 26 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of German chamomile oil application on alleviating atopic dermatitis-like immune alterations in mice

2010

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Historically, German chamomile (GC) oil has been used for treatment of skin disorders. BALB/c mice were sensitized twice a week with 100 µL of 1% 2,4-dinitrochlorobenzene (DNCB) and challenged twice the following week with 100 µL of 0.2% DNCB for atopic dermatitis induction. Thereafter, 3% GC oil was applied daily (70 µL, 6 times week) on the dorsal skin for 4 weeks. Saline or jojoba oil was used for the control mice. Blood was collected after second DNCB challenge, and at 2 and 4 weeks after initiating oil application. Serum IgE levels were significantly lowered in the GC oil application group at the end of the 4-week application period. The GC oil application for 4 weeks resulted in reduction in serum IgG1 level compared with that after 2-week application. The GC oil application group showed a significantly lower serum histamine level than the control group 2 weeks after oil application. Scratching frequency of the GC oil application group was significantly lower than either control groups. This study is to demonstrate GC oil's immunoregulatory potential for alleviating atopic dermatitis through influencing of Th2 cell activation.

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“…Single cell suspensions were prepared from spleens or mesenteric lymph nodes as described elsewhere [73] , [74] . Three mesenteric lymph nodes were collected from each mouse.…”

Section: Methodsmentioning

confidence: 99%

Aberrant Immune Responses in a Mouse with Behavioral Disorders

et al. 2011

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BTBR T+tf/J (BTBR) mice have recently been reported to have behaviors that resemble those of autistic individuals, in that this strain has impairments in social interactions and a restricted repetitive and stereotyped pattern of behaviors. Since immune responses, including autoimmune responses, are known to affect behavior, and individuals with autism have aberrant immune activities, we evaluated the immune system of BTBR mice, and compared their immunity and degree of neuroinflammation with that of C57BL/6 (B6) mice, a highly social control strain, and with F1 offspring. Mice were assessed at postnatal day (pnd) 21 and after behavioral analysis at pnd70. BTBR mice had significantly higher amounts of serum IgG and IgE, of IgG anti-brain antibodies (Abs), and of IgG and IgE deposited in the brain, elevated expression of cytokines, especially IL-33 IL-18, and IL-1β in the brain, and an increased proportion of MHC class II-expressing microglia compared to B6 mice. The F1 mice had intermediate levels of Abs and cytokines as well as social activity. The high Ab levels of BTBR mice are in agreement with their increased numbers of CD40hi/I-Ahi B cells and IgG-secreting B cells. Upon immunization with KLH, the BTBR mice produced 2–3 times more anti-KLH Abs than B6 mice. In contrast to humoral immunity, BTBR mice are significantly more susceptible to listeriosis than B6 or BALB/c mice. The Th2-like immune profile of the BTBR mice and their constitutive neuroinflammation suggests that an autoimmune profile is implicated in their aberrant behaviors, as has been suggested for some humans with autism.

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In Vitro Model for Modulation of Helper T Cell Differentiation and Activation

Cited by 5 publications

References 22 publications

Ameliorative effects of green tea extract from tannase digests on house dust mite antigen-induced atopic dermatitis-like lesions in NC/Nga mice

Ameliorative effects of green tea extract from tannase digests on house dust mite antigen-induced atopic dermatitis-like lesions in NC/Nga mice

Effect of German chamomile oil application on alleviating atopic dermatitis-like immune alterations in mice

Aberrant Immune Responses in a Mouse with Behavioral Disorders

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