In-Vitro Management of Phytohormones for Micropropagation of Sugarcane

Qazi, A. Q.; Nizamani, Ghulam Shah; Khan, Muhammad Tahir; Yasmeen, Shafquat; Baloch, Shahla Karim; Ali, M. A.; Khan, Imtiaz Ahmed; Ahmad, Sagheer; Nizamani, Muhammad Rashid; Siddiqui, M. A.

doi:10.17582/journal.pjar/2020/33.1.180.191

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“…The composition of hormones in the growth media plays crucial part. For shoot development BAP and Kinetin play vital role [2].…”

Section: Plant Height and Shootsmentioning

confidence: 99%

“…Propagation using in vitro techniques or tissue culture is one of the solutions in seed propagation of sugarcane plants because it can produce a lot of seeds, has the same genetics as the parent, does not require large areas of land, and is disease free, especially systemic diseases [1][2][3]. The application of in vitro culture techniques in seed multiplication from new superior varieties produced is useful for replacing old varieties with new varieties on a large scale.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Ten Genotypes of Sugarcane Mutants on Media Containing BA and Thidiazuron

Sukendah¹,

Purnamaningsih²,

Priyadarshini³

2023

Advances in Biological Sciences Research

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Mutation induction in sugarcane by gamma irradiation has produced 10 mutant clones. Each mutant clone needs to be reproduced before further release. Multiplication of mutant clones is done in-vitro to produce true-to-type mutant sugar cane seeds. Four in-vitro media containing BA (Benzylaminopurine) and Thidiazuron were designed to obtain the right media for each sugarcane mutant. The medium used was basic Murashige Skoog (MS) medium containing 100 mg/l PVP and 3% sucrose, with the addition of BA (1 and 3 mg/l) and thidiazuron (0 and 0.5 mg/l). The explants used were shoots from the ten genotypes of the sugarcane mutants, measuring ±0.5 cm. The study used a completely randomized design with observational variables: shoot height, number of shoots, number of roots, visual shoots and roots. The results showed that growth media containing 3 mg/l BA and 0.5 mg/l thidiazuron was the best medium for multiplication of mutant sugarcane. Growth regulator auxin NAA was more effective in inducing the formation of sugarcane roots. Clones K10 and K8 had the best shoot multiplication ability compared to other mutant clones.

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“…The composition of hormones in the growth media plays crucial part. For shoot development BAP and Kinetin play vital role [2].…”

Section: Plant Height and Shootsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%