In Vitro Interactions of Moroccan Propolis Phytochemical’s on Human Tumor Cell Lines and Anti-Inflammatory Properties

Abstract

Propolis is a resin manufactured by bees through the mixture of plant exudates and waxes with secreted substances from their metabolism, resulting in a complex mixture of natural substances of which quality depends on the phytogeographic and climatic conditions around the hive. The present study investigated the contribution of phenolic compounds to the cytotoxic and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (LC/DAD/ESI-MSn) analysis after phenolic extraction. The cytotoxicity of the extracts was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a composition rich in phenolic acids, such as caffeic and p-coumaric acid, as well as flavonoids, such as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3 from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4 from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity observed for sample MP2.

Unraveling the chemical composition, antioxidant, α-amylase and α-glucosidase inhibition of Moroccan propolis

“…The amount of resveratrol found in all analyzed samples was higher than that signaled by (Ristivojević et al, 2020) for Serbian, Turkish, Russian and Chinese propolis samples. Twenty-six phenolic compounds were previously detected in four Moroccan propolis samples, where pinobanksin, caffeic acid isoprenyl ester, isosakuranetin, caffeic acid, galangin and chrysin were the predominant phenolic components identified (Falcão et al, 2019). Thus, Moroccan propolis could have its place in the pharmaceutical revolution and the search for new natural drugs with high therapeutic efficacy.…”

Bioactive and Nutritional Potential of Medicinal and Aromatic Plant (MAP) Seasoning Mixtures

“…Subsequently, reviews on cancer prevention and treatment in animal and cell line models have been reflecting the effectiveness of phenolic compounds in inhibiting cancer development. Additionally, these compounds are described as having different mechanisms of action against tumor cells, namely through targeting human cell receptors, enzymes, transcription factors, and others [ 18 ]. Quercetin derivatives, widely occurring in plants and present in the studied extracts have been attracting further attention due to their anticancer activity, as well as antioxidant, antiviral, antibacterial, and anti-inflammatory effects [ 52 ].…”

“…In this field, recent studies emphasize the efficiency of MAPs extracts in the development of bakery and dairy products, with functional properties for consumers’ health improvement [ 13 , 14 , 15 ]. At a more specific level, these plants can also be used as complementary therapies in the treatment of diabetes mellitus, given their recognized antidiabetic activity through different mechanisms of action [ 16 ], in inhibiting the proliferation of tumor cells, targeting enzymes and DNA transcription factors [ 17 , 18 ], and being important allies in the maintenance of human immune system, due to the presence of compounds with recognized anti-inflammatory potential [ 19 ].…”

Avaliação da atividade anti-inflamatória de própolis de abelha Apis mellifera: uma revisão

“…O processo se inicia com a coleta das resinas vegetais pelos insetos, por meio de suas mandíbulas, e posterior transporte até a colmeia, utilizando a parte posterior da tíbia, chamada corbícula. No ninho, as abelhas acrescentam a esse material enzimas glandulares, como a β-13-glicosidase, cera e ácidos graxos-poliinsatudaros (Lungareze, 2010;Lima, 2012;Silva, 2012;Silva, Rodrigues, Feás & Estevinho, 2012;Búfalo, 2013;Falcão, 2013;El-Guendouz, et al, 2016;Salas, et al, 2016;Tiveron, et al, 2016;Banzato, 2018;Núñez, et al, 2018;Falcão, et al, 2019;Oliveira, 2020;Boeing, et al, 2021). Tais enzimas adicionadas têm como função a quebra de glicosídeos a agliconas (por exemplo, flavonas e flavanonas) (Alves, 2018).…”