In vitro interaction of usnic acid in combination with antimicrobial agents against methicillin-resistant Staphylococcus aureus clinical isolates determined by FICI and ΔE model methods

Segatore, Bernardetta; Bellio, Pierangelo; Setacci, Domenico; Brisdelli, Fabrizia; Piovano, Marisa; Garbarino, Juan A.; Nicoletti, Marcello; Amicosante, Gianfranco; Perilli, Mariagrazia; Celenza, Giuseppe

doi:10.1016/j.phymed.2011.10.012

Cited by 75 publications

(49 citation statements)

References 29 publications

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“…Many different biological properties, such as anti-inflammatory, antimicrobial, antioxidant, cytotoxic and antiproliferative activities, have been associated to lichen compounds [1][2][3][4]. In the recent years, the interest in the pharmaceutical potential of lichen metabolite [5,6] is increasing even if the biological activity and therapeutical potential of relatively few compounds have been deeply investigated.…”

Section: Introductionmentioning

confidence: 99%

Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

et al. 2016

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“…In regarding to toxicity, the authors also showed that usnic acid is safe in mice at concentrations of up to 100mg/kg. Segatore et al (2012) evaluated usnic acid in combination with antimicrobials and observed the following: synergism with gentamicin, antag- onism with levofloxacin, unresponsiveness when used in combination with erythromycin, and variability when associated with oxacillin and clindamycin. The authors suggested usnic acid as a good candidate for an antimicrobial agent when used in combination with other commercially available drugs.…”

Section: Determination Of Antibacterial Activity Of Organic Extract Omentioning

confidence: 99%

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

et al. 2017

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It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.

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“…Several studies have shown relevant pharmacological properties attributed to the usnic acid: Antibiotic (Segatore et al 2012, Pompilio et 1074KETSIA S.N. MARINHO et al al.…”

Section: Introductionmentioning

confidence: 99%

Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats

Marinho

Antonio

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to evaluate the teratogenic and hepatotoxic potential of the usnic acid encapsulated into PLGA-microspheres. In total, 12 female Wistar rats in pregnancy were randomly distributed in the control group (n= 6) that received 1.0 mL of physiological solution and treatment group (n= 6) that received 25 mg/kg of encapsulated usnic acid by oral administration. All females were euthanized at day 20 of pregnancy and their fetuses were removed and analyzed. During the pregnancy was observed a reduction in weight gain. There was no difference in serum transaminases levels analyzed as well as any difference in liver weight in both groups. The histomorphometric analysis of the liver from the treatment group revealed an increase in number of hepatocytes and a decrease in nuclear area of these cells. Moreover, no alteration was observed in cell area of hepatocytes or number of Kupffer cells. The fetuses had an increase in total number of hepatocytes and a reduction in the amount of megakaryocytes. These results show the hepatotoxic potential of usnic acid during pregnancy. However, its toxicity can be minimized by encapsulation in microspheres.

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In vitro interaction of usnic acid in combination with antimicrobial agents against methicillin-resistant Staphylococcus aureus clinical isolates determined by FICI and ΔE model methods

Cited by 75 publications

References 29 publications

Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

Hepatic toxicity caused by PLGA-microspheres containing usnic acid from the lichen C ladonia substellata (AHTI) during pregnancy in Wistar rats

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