Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

Brisdelli, Fabrizia; Perilli, Mariagrazia; Sellitri, Doriana; Bellio, Pierangelo; Bozzi, Argante; Amicosante, Gianfranco; Nicoletti, Marcello; Piovano, Marisa; Celenza, Giuseppe

doi:10.1016/j.lfs.2016.06.023

Cited by 19 publications

(8 citation statements)

References 38 publications

(41 reference statements)

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“…Among these drugs, docetaxel significantly increased the expression level of caspase-3 within 8 h, which is consistent as reported previously that docetaxel exhibited strong apoptotic induction effect both on HeLa cells and MCF-7 cells (Al-Abd et al, 2011). 5-FU and adriamycin induced the activation of caspase-3 at 48 h in Hela cells, which is similar to the former studies (Brisdelli et al, 2016;Mavrikou et al, 2019). In contrast, the induction of caspase-3 activity in MCF-7 cells by adriamycin was negligible relative to that in HeLa cells, while caspase-3 in MCF-7 cells was not activated by 5-FU stimulation.…”

Section: Discussionsupporting

confidence: 92%

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Gong

Wang

Abbas

et al. 2021

Sci. China Life Sci.

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Apoptosis is a form of programmed cell death that is essential for maintaining internal environmental stability. Disordered apoptosis can cause a variety of diseases; therefore, sensing apoptosis can provide help in study of mechanism of the relevant diseases and drug development. It is known that caspase-3 is a key enzyme involved in apoptosis and the expression of its activity is an indication of apoptosis. Here, we present a genetically encoded switch-on mNeonGreen2-based molecular biosensor. mNeonGreen2 is the brightest monomeric green fluorescent protein. The substrate of caspase-3, DEVD amino acid residues, is inserted in it, while cyclized by insertion of Nostoc punctiforme DnaE intein to abolish the fluorescence (inactive state). Caspase-3-catalyzed cleavage of DEVD linearizes mNeonGreen2 and rebuilds the natural barrel structure to restore the fluorescence (activated state). The characterization exhibited that the Caspase-3 biosensor has shortened response time, higher sensitivity, and prolonged functional shelf life in detection of caspase-3 amongst the existing counterparts. We also used the Caspase-3 biosensor to evaluate the effect of several drugs on the induction of apoptosis of HeLa and MCF-7 tumor cells and inhibition of Zika virus invasion. Supporting Information The supporting information is available online at 10.1007/s11427-021-1986-7. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Section: Discussionsupporting

confidence: 92%

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Gong

Wang

Abbas

et al. 2021

Sci. China Life Sci.

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“…Bačkorová et al [4] showed antiproliferative activity and induction of apoptosis mediated by well-known depside atranorin in wide spectrum of cancer cell lines. Promising results showed also treatments with another depside as lobaric acid [10], protolichesterinic acid [11], olivetoric acid [12] and physodic acids [13] with high anti-cancer potential.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Goga

Kello

Vilková

et al. 2019

BMC Complement Altern Med

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Background: Lichens produce a huge diversity of bioactive compounds with several biological effects. Gyrophoric acid (GA) is found in high concentrations in the common lichen Umbilicaria hirsuta, however evidence for biological activity was limited to anti-proliferative activity described on several cancer cell lines. Methods: We developed and validated a new protocol for GA isolation, resulting in a high yield of highly pure GA (validated by HPLC and NMR) in an easy and time saving manner. Anti-proliferative and pro-apoptotic activity, oxygen radicals formation and stress/survival proteins activity changes was study by flow cytometry. Results: The highly purified GA showed anti-proliferative activity against HeLa (human cervix carcinoma) and other tumor cells. Moreover, GA threated cells showed a significant increase in caspase-3 activation followed by PARP cleavage, PS externalization and cell cycle changes mediated by oxidative stress. Production of oxygen radicals led to DNA damage and changes in stress/survival pathways activation. Conclusions: GA treatment on HeLa cells clearly indicates ROS production and apoptosis as form of occurred cell death. Moreover, DNA damage and changing activity of stress/survival proteins as p38MAPK, Erk1/2 and Akt mediated by GA treatment confirm pro-apoptotic potential. The pharmacological potential of U. hirsuta derived GA is discussed.

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“…Bim expression, which mediates cytochrome-c release might be increased in an additive manner by both PLA and doxorubicin. Protolichesterinic acid also seems to behave as a competitive inhibitor of fatty acid synthase [82]. Bessadóttir et al also tested effects of PLA (from Cetraria islandica) in human multiple myeloma (RPMI 8226), (U266) and human pancreatic carcinoma (AsPC-1).…”

Section: In Vitro Evaluation Of Anticancer Efficacy Of Isolated Lichementioning

confidence: 99%

Anticancer Potential of Lichens’ Secondary Metabolites

et al. 2020

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Lichens produce different classes of phenolic compounds, including anthraquinones, xanthones, dibenzofuranes, depsides and depsidones. Many of them have revealed effective biological activities such as antioxidant, antiviral, antibiotics, antifungal, and anticancer. Although no clinical study has been conducted yet, there are number of in vitro and in vivo studies demonstrating anticancer effects of lichen metabolites. The main goal of our work was to review most recent published papers dealing with anticancer activities of secondary metabolites of lichens and point out to their perspective clinical use in cancer management.

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Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro

Cited by 19 publications

References 38 publications

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Anticancer Potential of Lichens’ Secondary Metabolites

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