In vitro inhibitory effects of asiaticoside and madecassoside on human cytochrome P450

Winitthana, Thidarat; Niwattisaiwong, Nuansri; Patarapanich, Chamnan; Tantisira, Mayuree H.; Lawanprasert, Somsong

doi:10.1016/j.tiv.2011.02.010

Cited by 17 publications

(11 citation statements)

References 8 publications

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“…For example, the inhibitory effects of asiaticoside and madecassoside on human CYPs were studied by Winitthana et al (2011). The results showed that asiaticoside inhibited CYP2C19 and CYP3A4.…”

Section: Resultsmentioning

confidence: 99%

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Purwantiningsih

Hussin

2014

Indonesian J. Pharm.

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Herbal remedies are often used concomitantly with prescribed medication, which leads to an increase in the potential of herb-drug interactions. Orthosiphon stamineus is one of the popular herbal preparations that is traditionally used, especially as a diuretic or for the elimination of kidney stones. The inhibitory effect of O. stamineus extract on human cytochrome P450 isoforms, namely CYP1A2, CYP3A4, CYP2C9 and CYP2D6, has been determined using luminescent methods. The final concentrations of O. stamineus extract tested were 0.01, 0.1, 1, 10, 100 and 1000µg/mL. IC 50 value was used to assess the modulation potencies for each CYP isoform. The extract showed a moderate inhibition towards CYP2C9 with the IC 50 of 20.12µg/mL and showed IC 50 of 49.90, 89.24 and 97.82µg/mL for CYP1A2, CYP2D6 and CYP3A4, respectively. These data suggest that O. stamineus extract may potentiate the herb-drug interaction via CYP inhibition.

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Section: Resultsmentioning

confidence: 99%

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Purwantiningsih

Hussin

2014

Indonesian J. Pharm.

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“…When samples were not preincubated, the IC 50 value of gypenosides was 1.85 lg/mL, whereas after preincubation, its IC 50 values were at 1.87 lg/mL and 2.53 lg/mL in without or with NADPH, respectively. A compound with a IC 50 ratio >1 was considered to be a mechanism-based inhibitor (Obach et al, 2007;Winitthana et al, 2011). Gypenosides was shown to be a possible non mechanismbased inhibitor of CYP2D6 (IC 50 ratio = 0.74).…”

Section: Determination Of the Inhibition Mode Of Gypenosidesmentioning

confidence: 99%

Inhibitory effects of gypenosides on seven human cytochrome P450 enzymes in vitro

Jiang

Qiu

et al. 2013

Food and Chemical Toxicology

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“…Aktivitas antiinflamasi dari ekstrak air herba pegagan dapat dikaitkan dengan adanya glikosida seperti asiaticoside dan madecassoside (George et al, 2009 (Winitthana et al, 2009). Enzim CYP450 tidak hanya terdapat di hati tetapi juga banyak terdapat di usus (Obach et al, 2000).…”

Section: Pendahuluanunclassified

“…Salah satu manfaat dari ekstrak air herba pegagan adalah memberikan efek antiinflamasi Winitthana et al, 2009;(George, 2009) sehingga dilakukan perlakuan ibuprofen bersama dengan ekstrak air herba pegagan untuk mengetahui perubahan parameter bioavailabilitas dari ibuprofen. Tampak gambar 1, bahwa pemberian ibuprofen bersama dengan ekstrak air herba pegagan baik kadar 25 %b/v, 50 %b/v maupun 100 %b/v dapat berpengaruh pada profil obat, pada umumnya kadar obat dalam darah mengalami peningkatan seiring meningkatnya konsentrasi ekstrak air herba pegagan yang diberikan, tetapi waktu untuk mendapatkan kadar puncak pada masing-masing perlakuan hampir sama.…”

Section: Penentuan Kadar Ibuprofen Pada Masing-masing Perlakuanunclassified

“…Salah satu zat aktif dari ekstrak air herba pegagan yaitu saponin jenis asiaticoside dan madecassoside yang memiliki aktifitas sebagai antiinflamasi ( (Zheng dan Qin, 2007;James and Dubery, 2009). Assiaticoside merupakan suatu inhibitor aktivitas enzim CYP450 (CYP3A4 dan CYP2C19) (Winitthana et al 2009). Enzim CYP450 banyak terdapat di dalam hati maupun usus (acuan).…”

Section: Penentuan Parameter Bioavailabilitas Ibuprofenunclassified

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BIOAVAILABILITAS TABLET IBUPROFEN PADA PEMBERIAN BERSAMAAN DENGAN EKSTRAK AIR HERBA PEGAGAN (Centella asiatica (L) Urban) PADA KELINCI JANTAN

Sutanti¹,

Wahyuningsih²

2013

Pharmaciana

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In vitro inhibitory effects of asiaticoside and madecassoside on human cytochrome P450

Cited by 17 publications

References 8 publications

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Inhibitory effects of gypenosides on seven human cytochrome P450 enzymes in vitro

BIOAVAILABILITAS TABLET IBUPROFEN PADA PEMBERIAN BERSAMAAN DENGAN EKSTRAK AIR HERBA PEGAGAN (Centella asiatica (L) Urban) PADA KELINCI JANTAN

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