In Vitro Inhibition of Cellulolytic Enzymes of Fusarium Oxysporum by Trichoderma spp and Pseudomonas Fluorescens on Arachis Hypogaea L

Abstract

In an attempt to develop biocontrol system for management of Fusarium wilt in groundnut, Trichoderma viride, Trichoderma harzianum,and Pseudomonas fluorescens were evaluated for their antagonistic activity against Fusarium oxysporum in vitro. .Fusarium wilt diseasescaused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtratesof T.viride (1%), T. harzianum (1.5%), and P. fluorescens (2%) on the in vitro inhibition of cellulolytic enzymes of Fusarium oxysporum. Theactivity of 1,4 endoglucanases, 1,4exoglucanase Cellobiase produced by Fusariumoxysporum was higher, when compared to control.Maximum inhibition of above Cellulolytic enzymes (1, 4 endoglucanases, 1,4exoglucanase, Cellobiase) was shown by T. viride treatment wasfollowed by T. harzianum and P. fluorescens. Of all the treatments, T. viride treatment showed higher rate of inhibition of Cellulolytic enzymesof Fusarium oxysporum followed by that of T. harzianum and P. fluorescens.This present study indicates that culture filtrate of T.viride(1%)is the best biocontrol agent in the inhibition of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea .LDOI: http://dx.doi.org/10.3126/ijasbt.v3i1.12138 Int J Appl Sci Biotechnol, Vol. 3(1): 106-110

Production, characterization and inhibition of fungal cellulases utilizing rice bran as substrate in solid state fermentation.

“…The flavonoid isolated from plants could be used as natural potential insecticide as they could bind the substrate binding site of the insect pest cellulases (red pumpkin beetle). The characterization and inhibition of a cellulase enzyme (endo-1, 4-beta-D-glucanase), was an example of repellency of insect pest by a plant derived flavonoid compound through inhibition of cellulases activity [25][26][27].…”

“…The culture filtrate of Trichoderma viride was reported as the one of biocontrol agent of Fusarium wilt disease caused by F. oxysporum [26]. Kaempfolienol, crotepoxide, boesenboxide, zeylenol, 2′-hydroxy-4, 4′, 6′-trimethoxychalcone, (24S)-24methyl-5α-lanosta-9, 25-dien-3β-ol, β-sitosterol and βsitosterol-3-O-β-D-glucopyranoside were isolated previously from narrow-leaved peacock ginger [18].…”

Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean

“…This fungus attacks at the germination phase with rot symptoms in the roots, hypocotyl, and cotyledons, causing damping-off and stunted growth therefore failure to germinate Cui et al 2016). F. oxysporum has also been reported to attack legumes (Husnain et al 2019), peanuts (Rajeswari 2015), and beans (Ellis et al 2013). Soybean yields could decrease by 22 kg ha -1 for every 1% increase of root rot disease caused by F. oxysporum (Maier et al 2018).…”

Antifungal properties of Beauveria bassiana strains against Fusarium oxysporum f. sp. lycopersici race 3 in tomato crop

“…The medium was prepared as described by Rajeswari (2015). Briefly, 50 ml adjusted to 1200 ml) in a 250 ml Erlenmeyer flasks and supplemented with crab shell chitin (1% w/v) as carbon source.…”

“…The B. bassiana strain 1215 was cultured on potato dextrose agar media at 28±2ºC for 15 days. Four disks of five mm diameter were cut and transferred into 50 ml flasks containing liquid medium (Rajeswari, 2015). The flasks were placed on a rotary shaker at 28 °C and 150 rpm for eight days (Ahmed Idris et al, 2007).…”