Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean

Sari, Maya Rizki; Nawangsih, Abdjad Asih; Wahyudi, Aris Tri

doi:10.13057/biodiv/d220602

Cited by 15 publications

(7 citation statements)

References 28 publications

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“…This could be attributed to compromised defense mechanisms of the susceptible cultivar, allowing opportunistic pathogens to colonize and proliferate, or possibly due to speci c root exudates from this cultivar that inadvertently promote the growth of these pathogens. The exclusivity of Streptomyces panaciradicis, Pseudomonas uorescens, and Streptomyces griseoplanus in the SDS-tolerant rhizosphere may also re ect host selection, as the two former have been leveraged as biocontrol agents against Fusarium pathogens [151,152] and the latter as a biocontrol agent against Macrophomina [153]. The Pseudomonas genus has also been associated with SDS-suppressive soils spanning 45 soybean elds [10].…”

Section: Discussionmentioning

confidence: 99%

Single-molecule-based characterization of the soybean rhizosphere microbiome

Hale,

Watts,

Conatser

et al. 2024

Preprint

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Background The rhizosphere microbiome displays structural and functional dynamism driven by plant, microbial, and environmental factors. While such plasticity is a well-evidenced determinant of host health, individual and community-level microbial activity within the rhizosphere remain poorly understood, due in part to the insufficient taxonomic resolution achieved through traditional marker gene amplicon sequencing. This limitation necessitates more advanced approaches (e.g., long-read sequencing) to derive ecological inferences with practical application. To this end, the present study coupled synthetic long-read technology with avidity sequencing to investigate eukaryotic and prokaryotic microbiome dynamics within the soybean (Glycine max) rhizosphere under field conditions. Results Synthetic long-read sequencing permitted de novo reconstruction of the entire 18S-ITS1-ITS2 region of the eukaryotic rRNA operon as well as all nine hypervariable regions of the 16S rRNA gene. All mapped eukaryotic amplicon sequence variants displayed genus-level classification, and 44.77% achieved species-level classification. The resultant eukaryotic microbiome encompassed five kingdoms (19 genera) of protists in addition to fungi – a depth unattainable with conventional short-read methods. In the prokaryotic fraction, every mapped amplicon sequence variant was resolved at the species level, and 23.13% at the strain level. Thirteen species of Bradyrhizobium were thereby distinguished in the prokaryotic microbiome, with strain-level identification of the two Bradyrhizobium species most reported to nodulate soybean. Moreover, the applied methodology delineated structural and compositional dynamism in response to experimental parameters (i.e., growth stage, cultivar, and biostimulant application), unveiled a saprotroph-rich core microbiome, provided empirical evidence for host selection of mutualistic taxa, and identified key microbial co-occurrence network members likely associated with edaphic and agronomic properties. Conclusions This study is the first to combine synthetic long-read technology and avidity sequencing to profile both eukaryotic and prokaryotic fractions of a plant-associated microbiome. Findings herein provide an unparalleled taxonomic resolution of the soybean rhizosphere microbiota and represent significant biological and technological advancements in crop microbiome research.

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Section: Discussionmentioning

confidence: 99%

Single-molecule-based characterization of the soybean rhizosphere microbiome

Hale,

Watts,

Conatser

et al. 2024

Preprint

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“…In a different study, Streptomyces atratus and S. tsukiyonensis were effective against brown rot fungus, Gloeophyllum trabeum , and protected the wood properties [ 43 ]. When the Trichoderma strains (#5001 and #5029) and streptomycetes (#USC-6914 and #USC-595-B) were used together, possibly the combined biological control mechanisms [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ] of them resulted in the induced antagonistic activity against the pathogen and provided better protection than the single BCA application. Therefore, these BCAs can be used together as an effective management tool to protect wood decay from P. noxium .…”

Section: Discussionmentioning

confidence: 99%

Testing the Biocontrol Ability of a Trichoderma-Streptomycetes Consortium against Pyrrhoderma noxium (Corner) L.W. Zhou and Y.C. Dai in Soil

Panchalingam

Ashfield-Crook

Naik³

et al. 2022

JoF

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The Brown root rot pathogen Pyrrhoderma noxium (Corner) L.W. Zhou and Y.C. Dai is known to infect a large number of culturally and economically important plant species across the world. Although chemical control measures have been effective in managing this pathogen, their adverse effects on the ecosystem have limited their use. The use of biological control agents (BCAs) thus is generally accepted as an environmentally friendly way of managing various pathogens. Testing various consortia of the BCAs with different antagonistic mechanisms may even provide better disease protection than the use of a single BCA against aggressive plant pathogens such as the P. noxium. In the presented study, the wood decay experiment and the pot trial confirmed that the consortium of Trichoderma strains (#5029 and 5001) and streptomycetes (#USC−6914 and #USC−595-B) used was effective in protecting wood decay and plant disease caused by P. noxium. Among the treatments, complete elimination of the pathogen was observed when the BCAs were applied as a consortium. In addition, the BCAs used in this study promoted the plant growth. Therefore, Trichoderma and streptomycetes consortium could be used as a potential biocontrol measure to manage P. noxium infections in the field over the application of hazardous chemical control measures.

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“…The dry actinomycetes cells were weighed and compared with the control filter paper. The best alternative medium resulting in the highest actinomycetes biomass was used for further actinomycete culture in the formulation stage (Sari et al 2021).…”

Section: Optimization Of Alternative Media For Actinomycete Cell Prop...mentioning

confidence: 99%

“…Next, 100 µL of each dilution was plated into a Petri dish containing ISP2 agar and incubated for five days at ± 25°C. The number of actinomycetes colonies was counted according to (Sari et al 2021).…”

Section: Evaluation Of Actinomycetes Viability In Various Carrier Mat...mentioning

confidence: 99%

“…Actinomycetes colonize the rhizosphere and interact with plants by producing growth-promoting compounds that can inhibit the growth of phytopathogens and promote plant growth (Boukhatem et al 2022). Earlier studies reported that actinomycetes, especially the Streptomyces group, from soil and rhizosphere have activity in increasing plant growth and inhibiting the spread of soil-borne phytopathogens, such as Fusarium oxysporum (Mariastuti et al 2018;Sari et al 2021) Rhizoctonia solani (Ebrahimi-Zarandi et al 2021) and Verticillium dahliae (Xue et al 2013). Furthermore, in several developed countries such as the United States, Canada, Finland, and New Zealand, biofertilizer agents and actinomycetes-based biocontrol of plant pathogenic bacteria and fungi have been used (Palaniyandi et al 2013a).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of culture medium and bioformulation of rhizobial actinomycetes to enhance soybean plant growth

FATMAWATI,

QURRATA’AINI,

ROSYIDI

et al. 2023

Biodiversitas

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Abstract. Fatmawati U, Qurrata’aini MR, Rosyidi NW, Sari DP, Santosa S. 2023. Optimization of culture medium and bioformulation of rhizobial actinomycetes to enhance soybean plant growth. Biodiversitas 24: 2912-2918. Actinomycetes are a group of Gram-positive bacteria that are well-known for having plant growth-promoting activity. In an earlier study, Streptomyces sp. strain ASR 58 and Streptomyces sp. strain ASR 67, isolated from soybean rhizosphere, were found to be capable of producing Indole-Acetic Acid (IAA), dissolving phosphate, and stimulating soybean growth. Optimization of culture media and selection of carrier materials for formulation are necessary to maintain actinomycetes' cell viability and plant growth property stability. This study aimed to evaluate several culture media, determine the optimal carrier materials in actinomycetes formulation, and evaluate the effect of actinomycete bioformulation on the soybean plant growth in planta. The sugar potato broth medium was the best in increasing the cell biomass of ASR 67 and co-culture of ASR 58 and ASR 67 compared to other mediums (skim molasses and rice bran broth). The viability of ASR 58 cell (5.3 x 107 CFU/mL), ASR 67 (3.8 x 107 CFU/mL), and its combination of both (4.3 x 107 CFU/mL) was best maintained in talc powder carrier during 10 weeks of storage as revealed by the number of viable cells decreases. Applying ASR 58 and ASR 67 co-culture on talc powder formula enhanced soybean plant biomass four weeks after planting, including plant height, the number of leaves, and root dry weight.

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Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean

Cited by 15 publications

References 28 publications

Single-molecule-based characterization of the soybean rhizosphere microbiome

Single-molecule-based characterization of the soybean rhizosphere microbiome

Testing the Biocontrol Ability of a Trichoderma-Streptomycetes Consortium against Pyrrhoderma noxium (Corner) L.W. Zhou and Y.C. Dai in Soil

Optimization of culture medium and bioformulation of rhizobial actinomycetes to enhance soybean plant growth

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