In vitro IFN-gamma production by goat blood cells after stimulation with somatic and secreted Corynebacterium pseudotuberculosis antigens

Meyer, Roberto; Regis, Lia; Vale, Vera Lúcia Costa; Paule, Bruno Jean Adrien; Carminati, Renato; Bahia, Robson; Moura-Costa, Lília Ferreira de; Schaer, Robert; Nascimento, Ivana; Freire, Songelí Menezes

doi:10.1016/j.vetimm.2005.05.002

Cited by 22 publications

(20 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A commercial kit to perform a more reliable identification was also used (API Coryne - bioMérieux). According to previous studies, this strain can’t induce disease in goats [37,38] and mice [8], and was used as a vaccinal strain [39]. Furthermore, during the synergistic hemolysis test, we observed a pattern of less severe hemolysis when compared with other wild strains.…”

Section: Methodsmentioning

confidence: 67%

See 1 more Smart Citation

MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

et al. 2014

View full text Add to dashboard Cite

BackgroundCaseous lymphadenitis (CL) is a contagious infectious disease of small ruminants caused by Corynebacterium pseudotuberculosis. Is characterized by the formation of abscesses in the lymph nodes and intestines of infected animals, induced by inflammatory cytokines. The production of cytokines, such as IL-10, TNF-α, IL-4 and IFN-γ, is regulated by mitogen-activated protein kinase (MAPK) pathway activation. The present study investigated the involvement of MAPK pathways (MAPK p38, ERK 1 and ERK 2) with respect to the production of cytokines induced by antigens secreted by C. pseudotuberculosis over a 60-day course of infection. CBA mice (n = 25) were divided into three groups and infected with 102 colony forming units (CFU) of attenuated strain T1, 102 CFU of virulent strain VD57 or sterile saline solution and euthanized after 30 or 60 days. Murine splenocytes were treated with specific inhibitors (MAPK p38 inhibitor, ERK 1/2 inhibitor or ERK 2 inhibitor) and cultured with secreted antigens obtained from pathogenic bacteria (SeT1 or SeVD57).ResultsThe MAPK pathways evaluated were observed to be involved in the production of IL-10, under stimulation by secreted antigens, while the MAPK p38 and ERK 1 pathways were shown to be primarily involved in TNF-α production. By contrast, no involvement of the MAPK p38 and ERK 1 and 2 pathways was observed in IFN-γ production, while the ERK 2 pathway demonstrated involvement in IL-4 production only in the mouse splenocytes infected with VD57 under stimulation by SeT1.ConclusionThe authors hypothesize that MAPK p38 and ERK 1 pathways with respect to TNF-α production, as well as the MAPK p38 and ERK 1 and 2 pathways in relation to IL-10 production under infection by C. pseudotuberculosis are important regulators of cellular response. Additionally, the lack of the MAPK p38 and ERK 1/2 pathways in IFN-γ production in infected CBA murine cells stimulated with the two secreted/excreted antigens, in IL-4 production showing involvement only via the ERK 2 pathway under stimulation by SeT1 antigen during 60-day infection period with the virulent strain, suggests that these pathways regulated the production of pro-inflammatory and regulatory cytokines in the splenic cells of CBA mice.

show abstract

Section: Methodsmentioning

confidence: 67%

“…This strain induces caseous lymphadenitis in goats [38–40] and mice [8]. The bacteria were grown in the ICS-UFBA microbiology department laboratory in Brain Heart Infusion (BHI) broth.…”

Section: Methodsmentioning

confidence: 99%

MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The present study employed a naturally attenuated strain of C. pseudotuberculosis , denominated T1, isolated from a granuloma taken from a goat in a rural region of the state of Bahia, located in northeastern Brazil. Studies previously conducted with this strain show that it grows quickly in BHI broth medium, when compared to other strains, but is incapable of inducing disease in goats [22–24]. …”

Section: Introductionmentioning

confidence: 99%

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

et al. 2016

View full text Add to dashboard Cite

BackgroundCorynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.ResultsThe lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.ConclusionThe T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

show abstract

“…17 Use of this assay in goats after stimulation with secreted and somatic antigens from C. pseudotuberculosis has also been described. 12 Secreted antigens in a chemically defined medium 15 presented an increased stimulation, with higher production of IFN-c, when compared with the stimulation induced by a somatic antigen. However, the weak induction of IFN-c by a somatic antigen has not been observed by other researchers 11,18,20 ; this discrepancy can be explained by the utilization of different pathogenic, attenuated, or genetically modified strains, 17 or because of different antigen purification methods.…”

Section: Discussionmentioning

confidence: 98%

“…6,28 Because C. pseudotuberculosis is a facultative intracellular pathogen, cell-mediated immunity is an important component of the protective immune response. 9 Previously, a whole-blood gamma interferon (IFN-c) assay was found to be a promising detection tool for the eradication of CLA from small ruminant flocks, 11,12,24 An alternative approach to the immunodiagnosis of CLA is the assessment of the cellular immune response to C. pseudotuberculosis antigens, 11,12,20,24 carried out concurrently with serologic assays.…”

Section: Introductionmentioning

confidence: 99%

Corynebacterium Pseudotuberculosis Secreted Antigen-Induced Specific Gamma-Interferon Production by Peripheral Blood Leukocytes: Potential Diagnostic Marker for Caseous Lymphadenitis in Sheep and Goats

Rebouças¹,

Portela²,

Lima³

et al. 2011

J VET Diagn Invest

View full text Add to dashboard Cite

In the current study, the applicability of the quantification of gamma interferon (IFN-γ) levels for the detection of animals infected with Corynebacterium pseudotuberculosis and for determining caseous lymphadenitis (CLA) clinical status was evaluated. Peripheral blood leukocytes were collected from CLA nonendemic areas animals, from CLA seropositive animals without clinical signs of the disease, and from seropositive animals presenting CLA clinical signs. The leukocytes were stimulated with C. pseudotuberculosis-secreted antigens that were concentrated by the three-phase partitioning technique. An ovine IFN-γ enzyme-linked immunosorbent assay was used to quantify IFN-γ production. Goats and sheep with CLA had higher IFN-γ levels than uninfected seronegative animals. Leukocytes from sheep with CLA chronic abscesses produced higher IFN-γ levels when compared with seropositive sheep without CLA clinical signs, but this difference was not significant in goats. The sensitivity of the assay was 55.8% and 56%, whereas the specificity was 100% and 93%, for goats and sheep, respectively. In conclusion, IFN-γ is a potential marker for the determination of CLA infection status in small ruminants; however, further research is needed to improve assay sensitivity.

show abstract

In vitro IFN-gamma production by goat blood cells after stimulation with somatic and secreted Corynebacterium pseudotuberculosis antigens

Cited by 22 publications

References 10 publications

MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

Corynebacterium Pseudotuberculosis Secreted Antigen-Induced Specific Gamma-Interferon Production by Peripheral Blood Leukocytes: Potential Diagnostic Marker for Caseous Lymphadenitis in Sheep and Goats

Contact Info

Product

Resources

About