Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

Vale, Vera Lúcia Costa; Silva, Marcos da Costa; Souza, Andréia Pacheco de; Trindade, Soraya Castro; Moura-Costa, Lília Ferreira de; Santos-Lima, Ellen Karla Nobre dos; Nascimento, Ivana; Saba, Hugo; Marques, Edson de Jesus; Paule, Bruno Jean Adrien; Nascimento, Roberto José Meyer

doi:10.1186/s12917-016-0811-8

Cited by 14 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest increased in Group B (toxoid PLD+bacterin) little followed by Group A (Toxoid PLD alone), then Groups D and C nearly similar response. Furthermore, near results obtained in Table-2 showed that the significant lysozyme activity of simulated macrophage was the highest increased in Group B followed by Group A, then C and the least one was represented in Group D. These outcomes were consistent with those of other studies [ 24 , 25 ] and may be attributed to the presence of somatic proteins associated with bacterin which induce the production of IFN-γ, a very important cytokine related to innate immune response and macrophage stimulation [ 26 ].…”

Section: Resultssupporting

confidence: 91%

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged

et al. 2018

View full text Add to dashboard Cite

Background:Caseous lymphadenitis (CLA) is a serious disease affects sheep and goat, caused by Corynebacterium pseudotuberculosis. Due to it is non-treatable disease, so the effective preventive vaccines are considered a significant way to combat the disease. All strains of C. pseudotuberculosis have several virulence factors that associated with their cell invasion, survival, and proliferation such as phospholipase D (PLD), outer lipid coat, and secreted proteases.Aim:The present study was directed to perform a comparative innate and acquired immune response assessment of different four vaccine formulas to evoke protection against induced (CLA) challenge in sheep.Materials and Methods:Negative ELISA (free of CLA) 15 local breed male (Balady) sheep were divided into five groups, each has received a different vaccine while the control has received saline buffer. The first vaccine composed of toxoid PLD alone the second composed of toxoid PLD with bacterin (formalinkilled bacteria), the third vaccine composed of toxoid PLD plus covaccine 8, while the fourth one composed of toxoid PLD plus locally produced polyvalent clostridial vaccine. The specific immune response was evaluated through lymphocyte proliferation assay using ELISA BrdU kit, while the non-specific response was estimated by superoxide anion production and lysozyme activity assays.Results:The study revealed that PLD toxoid could evoke the highest specific immune response, showing a stimulation index (9.12%). On the other hand, combined toxoid ↱PLD with bacterin followed by PLD toxoid showed a significant increase in the non-specific innate immune response.Conclusion:The present study indicated that the toxoid PLD alone vaccine was most efficient and provided innate and acquired immune response in animals against CLA.

show abstract

Section: Resultssupporting

confidence: 91%

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Since C. pseudotuberculosis is a facultative intracellular pathogen, the protective immune response to the bacterium is based on a Th1 cellular response, and the infection with virulent strains of the bacteria induces the production of Th1 response-related cytokines such as IFN-gamma (Meyer et al, 2005 ; Vale et al, 2016 ). Indeed, it was seen that the production of IFN-gamma and TNF following the stimulation of murine splenocytes with the bacterium is driven by the activation of MAPK p38 and ERK 1/2 signaling pathways (de Souza et al, 2014 ).…”

Section: Virulence Factorsmentioning

confidence: 99%

“…The humoral immune response is also activated in the infection by C. pseudotuberculosis . In a murine model, it was observed that there is a peak in the production of IgG1 and IgG2a 60 days after an experimental infection with a pathogenic strain of the bacteria (Vale et al, 2016 ). Recent experiments on the development of vaccines against the bacteria showed that the production of IgG2a is correlated with a higher protection against a virulent challenge in mice that were previously inoculated with C. pseudotuberculosis recombinant proteins (Silva et al, 2014 ; Brum et al, 2017 ).…”

Section: Virulence Factorsmentioning

confidence: 99%

Insight of Genus Corynebacterium: Ascertaining the Role of Pathogenic and Non-pathogenic Species

Oliveira

Aburjaile

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

This review gathers recent information about genomic and transcriptomic studies in the Corynebacterium genus, exploring, for example, prediction of pathogenicity islands and stress response in different pathogenic and non-pathogenic species. In addition, is described several phylogeny studies to Corynebacterium, exploring since the identification of species until biological speciation in one species belonging to the genus Corynebacterium. Important concepts associated with virulence highlighting the role of Pld protein and Tox gene. The adhesion, characteristic of virulence factor, was described using the sortase mechanism that is associated to anchorage to the cell wall. In addition, survival inside the host cell and some diseases, were too addressed for pathogenic corynebacteria, while important biochemical pathways and biotechnological applications retain the focus of this review for non-pathogenic corynebacteria. Concluding, this review broadly explores characteristics in genus Corynebacterium showing to have strong relevance inside the medical, veterinary, and biotechnology field.

show abstract

“…Immunity to C. pseudotuberculosis is attributed to humoral and cellular mechanisms (Lan et al, 1998). Humoral immunity is important in the fight against C. pseudotuberculosis since antibodies neutralize bacterial exotoxins, avoiding their dissemination in the host (Vale et al, 2016). The results obtained in this study regarding the detection of anti- C pseudotuberculosis specific antibodies, for both sheep and goats, demonstrated a higher production of these antibodies in the iodine-treated group of animals than in the AgNP-treated animals during the initial period of analysis.…”

Section: Discussionmentioning

confidence: 99%

Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

et al. 2019

View full text Add to dashboard Cite

Caseous lymphadenitis (CL) is an infectious and zoonotic disease characterized by the development of granulomas in the lymph nodes and internal organs of small ruminants. The etiological agent of this disease is Corynebacterium pseudotuberculosis , a Gram-positive and facultative intracellular bacterium. The conventional treatment for CL consists of drainage and chemical cauterization of the lesions using a 10% iodine solution. However, this type of treatment is not effective, due to iodine’s cytotoxic profile and low antibacterial activity. Currently, silver nanoparticles (AgNPs) can be seen as an alternative treatment for CL due to their antimicrobial activity and wound healing effects. Therefore, the present study aimed to evaluate AgNPs as a post-surgical treatment for CL. Twenty-nine goats and sheep with clinical signs of CL were selected. Surgical intervention was performed to excise the caseous lesions. To treat the lesions, an ointment formulation based on AgNP mixed with natural waxes and oils was used in the experimental group, and the conventional treatment with 10% iodine was used in the control group. Bacteria were isolated from the excised caseous material. The animals were observed for 8 weeks after the surgical treatment, and blood samples were taken weekly. The surgical wounds of sheep treated with AgNP healed faster, and the surgical wound area was smaller during the observation period; the latter effect was also observed in goats. AgNP-treated animals also had less purulent discharge and less moisture in the surgical wounds. The AgNP-treated animals had lower leukocyte counts and lower titers of anti- C. pseudotuberculosis antibodies. There was no statistically significant difference between the groups with regard to the hemogram results. The results of the susceptibility testing of C. pseudotuberculosis strains (T1, 1002, FRC41, and VD57 strains) and clinical isolates to AgNPs showed growth inhibition, even at low concentrations. It can be concluded that post-surgical treatment of CL using the AgNP-based ointment may be a promising tool in the control of CL, through faster healing, decreased wound contamination, and no apparent toxic effects.

show abstract

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

Cited by 14 publications

References 36 publications

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged

Insight of Genus Corynebacterium: Ascertaining the Role of Pathogenic and Non-pathogenic Species

Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

Contact Info

Product

Resources

About