In Vitro Identification of Gold Nanorods through Hyperspectral Imaging

Stacy, Bradley M.; Hussain, Saber M.; Comfort, Kristen K.; Comfort, Donald A.

doi:10.21236/ada582417

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…Dark-field microscopy is used to characterize the expression of scattered light from Au NPs that can be utilized to visualize Au NPs in living cells. 30,31 As shown in Figure 3c, it was obvious the cellular uptake level of Au NPs in DMEM was much higher than that in c-DMEM by both cell lines, and much more Au NPs were internalized by RAW 264.7 cells than Hep G2 cells.…”

Section: ■ Results and Discussionmentioning

confidence: 95%

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cheng

Tian

et al. 2015

ACS Appl. Mater. Interfaces

255

212

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The interaction at nanobio is a critical issue in designing safe nanomaterials for biomedical applications. Recent studies have reported that it is nanoparticle-protein corona rather than bare nanoparticle that determines the nanoparticle-cell interactions, including endocytic pathway and biological responses. Here, we demonstrate the effects of protein corona on cellular uptake of different sized gold nanoparticles in different cell lines. The experimental results show that protein corona significantly decreases the internalization of Au NPs in a particle size- and cell type-dependent manner. Protein corona exhibits much more significant inhibition on the uptake of large-sized Au NPs by phagocytic cell than that of small-sized Au NPs by nonphagocytic cell. The endocytosis experiment indicates that different endocytic pathways might be responsible for the differential roles of protein corona in the interaction of different sized Au NPs with different cell lines. Our findings can provide useful information for rational design of nanomaterials in biomedical application.

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Section: ■ Results and Discussionmentioning

confidence: 95%

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cheng

Tian

et al. 2015

ACS Appl. Mater. Interfaces

255

212

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“…To date, gold NPs (AuNPs) are among the most frequently investigated and utilized materials. Due to their unique plasmonic properties, general biocompatibility, and ease of functionalization, AuNPs have distinguished themselves as a leading candidate for a vast number of biomedical applications, including photothermal therapy, drug delivery, and enhanced bio-imaging [ 2 , 3 ]. However, coinciding with this growth in NP utilization is an increased likelihood of unintentional material exposure, with yet unresolved biological consequences.…”

Section: Introductionmentioning

confidence: 99%

The role of biological fluid and dynamic flow in the behavior and cellular interactions of gold nanoparticles

2015

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BackgroundDue to their distinctive physicochemical properties, nanoparticles (NPs) have proven to be extremely advantageous for product and application development, but are also capable of inducing detrimental outcomes in biological systems. Standard in vitro methodologies are currently the primary means for evaluating NP safety, as vast quantities of particles exist that require appraisal. However, cell-based models are plagued by the fact that they are not representative of complex physiological systems. The need for a more accurate exposure model is highlighted by the fact that NP behavior and subsequent bioresponses are highly dependent upon their surroundings. Therefore, standard in vitro models will likely produce inaccurate NP behavioral analyses and erroneous safety results. As such, the goal of this study was to develop an enhanced in vitro model for NP evaluation that retained the advantages of cell culture, but implemented the key physiological variables of accurate biological fluid and dynamic flow.ResultsIn this study, a cellular microenvironment was modeled and created after an inhalation exposure scenario. This system comprised of A549 lung epithelial cells, artificial alveolar fluid (AAF), and biologically accurate dynamic flow. Under the influence of microenvironment variables, tannic acid coated gold NPs (AuNPs) displayed modulated physicochemical characteristics, including increased agglomeration, disruption of the spectral signature, and decreased rate of ionic dissolution. Furthermore, AuNP deposition efficiency, internalization patterns, and the nano-cellular interface varied as a function of fluid composition and flow condition. AAF incubation simultaneously influenced both AuNPs and cellular behavior, through excessive NP agglomeration and alteration to A549 morphology. Dynamic flow targeted the nano-cellular interface, with differential responses including modified deposition, internalization patterns, and cellular elongation. Lastly, the biocompatibility of the system was verified to ensure cellular health following AAF exposure and fluid dynamics.ConclusionsThis study confirmed the feasibility of improving standard in vitro models through the incorporation of physiological variables. Utilization of this enhanced system demonstrated that to elucidate true NP behavior and accurately gauge their cellular interactions, assessments should be carried out in a more complex and relevant biological exposure model.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0117-1) contains supplementary material, which is available to authorized users.

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“…To explore the cellular uptake of DOX-TSMLs-AuNSs-PEG with or without magnetic targeting by HepG2 cells, we have tracked the delivery system internalization into the cells through colocalization of the Au signal (golden yellow fluorescence). Au was successfully identified in vitro through their spectral signature alone . As shown in Figure A∼C, HepG2 cells incubated with DOX-TSMLs-AuNSs-PEG for different periods of time clearly revealed the time-dependent cellular uptake manner.…”

Section: Resultsmentioning

confidence: 93%

Radiofrequency-Triggered Tumor-Targeting Delivery System for Theranostics Application

Wang

Zhang

Shi

et al. 2015

ACS Appl. Mater. Interfaces

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In this study, a new type of magnetic tumor-targeting PEGylated gold nanoshell drug delivery system (DOX-TSMLs-AuNSs-PEG) based on doxorubicin-loaded thermosensitive magnetoliposomes was successfully obtained. The reverse-phase evaporation method was used to construct the magnetoliposomes, and then gold nanoshells were coated on the surface of it. The DOX-TSMLs-AuNSs-PEG delivery system was synthesized after SH-PEG2000 modification. This multifunction system was combined with a variety of functions, such as radiofrequency-triggered release, chemo-hyperthermia therapy, and dual-mode magnetic resonance/X-ray imaging. Importantly, the DOX-TSMLs-AuNSs-PEG complex was found to escape from endosomes after cellular uptake by radiofrequency-induced endosome disruption before lysosomal degradation. All results in vitro and in vivo indicated that DOX-TSMLs-AuNSs-PEG is a promising effective drug delivery system for diagnosis and treatment of tumors.

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In Vitro Identification of Gold Nanorods through Hyperspectral Imaging

Cited by 5 publications

References 27 publications

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

The role of biological fluid and dynamic flow in the behavior and cellular interactions of gold nanoparticles

Radiofrequency-Triggered Tumor-Targeting Delivery System for Theranostics Application

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