Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cheng, Xiaju; Tian, Xin; Wu, Anqing; Li, Jianxiang; Tian, Jian; Chong, Yu; Chai, Zhifang; Yang, Zhao; Chen, Chunying; Ge, Cuicui

doi:10.1021/acsami.5b04290

Cited by 256 publications

(226 citation statements)

References 34 publications

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“…HP and HSA coronas decreased hepatocyte uptake of all bare 40 and 80 nm BPEI-and LA-AuNP and 80 nm PEG-AuNP at 12 h and 24 h, but showed an increase in cell uptake for 40 nm PEG-AuNP ( Figure 4(A-F)). An inhibitory effect of serum coronas on the uptake of AuNP with hexadecyltrimethyl ammonium bromide-Au nanorods and poly (4-styrene sulfonic acid)-Au-nanorods in HeLa cells (Hauck et al, 2008); and citrate-AuNP in HepG2 and RAW 264.7 (Cheng et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Human serum albumin (HSA) and IgG coronas enhanced cellular uptake of citrate coated AuNP in human umbilical vein endothelial cells (HUVEC) compared to fibrinogen . Serum coated AuNP reduced NP cellular uptake in HepG2 and murine macrophages (Cheng et al, 2015). AuNP with tannic acid inhibited CYP2C9, CYP2C19, CYP2D6 and CYP3A4 activities in human liver microsomes (Ye et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

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Protein corona modulation of hepatocyte uptake and molecular mechanisms of gold nanoparticle toxicity

2016

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Protein corona formation over gold nanoparticles (AuNP) can modulate cellular responses by altering AuNP physicochemical properties. The liver plays an essential role in metabolism, detoxification and elimination of xenobiotics and drugs as well as circulating NP clearance. We investigated human hepatic uptake of 40 and 80 nm AuNP with branched polyethylenimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings as well as human plasma protein (HP) and human serum albumin (HSA) coronas. AuNP-mediated cytotoxicity, reactive oxygen/reactive nitrogen species (ROS/RNS), and CYP activity in human hepatocytes as well as molecular mechanisms with 40 nm bare and HP BPEI-AuNP were investigated. Time-dependent increase in uptake occurred for all bare AuNP but HP and HSA decreased uptake except for 40 nm HP PEG-AuNP. BPEI-AuNP showed time-and concentration-dependent increase in ROS/ RNS which correlated with cytotoxicity at 24 h. HP corona substantially reduced ROS/RNS. The 40 and 80 nm bare, HP or HSA LA-and PEG-AuNP were not toxic but HP was as cytotoxic as bare BPEI-AuNP. All bare and HP BPEI-AuNP, except for HP 80 nm BPEI-AuNP toward CYP1A2, inhibited CYP1A2, CYP2C9 and CYP3A4 activity. Transcriptional profiling was dose-dependent with 40 nm bare BPEI-AuNP (1.9% genes at IC 10 and 18.9% at LC 50 ) and HP (23.5% at LC 50 ). Differentially expressed genes at LC 50 were mainly involved in phase I metabolism and phospholipidosis pathways. Cytotoxicity of bare BPEI-AuNP caused an upregulation of antioxidant and pro-apoptotic genes. These studies contribute to a better understanding of the dramatic effect of protein coronas (PC) on AuNP cellular uptake, cytotoxicity and their underlying molecular mechanisms of action. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Protein corona modulation of hepatocyte uptake and molecular mechanisms of gold nanoparticle toxicity

2016

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“…Several studies investigated the role of either size, surface chemistry or the presence of a protein corona on cell uptake of AuNP (Cheng et al, 2015;Choi et al, 2017;Freese et al, 2012;Li & Monteiro-Riviere, 2016;Mirsadeghi et al, 2015;Monteiro-Riviere et al, 2013;Sasidharan et al, , 2016. However, there is lack of information on the definite links between the NP physicochemical properties, signature biocorona adsorbomes, cell uptake patterns, toxicity and gene expression profiles.…”

Section: Introductionmentioning

confidence: 99%

Surface chemistry of gold nanoparticles determines the biocorona composition impacting cellular uptake, toxicity and gene expression profiles in human endothelial cells

2017

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This study investigated the role of nanoparticle size and surface chemistry on biocorona composition and its effect on uptake, toxicity and cellular responses in human umbilical vein endothelial cells (HUVEC), employing 40 and 80 nm gold nanoparticles (AuNP) with branched polyethyleneimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings. Proteomic analysis identified 59 hard corona proteins among the various AuNP, revealing largely surface chemistry-dependent signature adsorbomes exhibiting human serum albumin (HSA) abundance. Size distribution analysis revealed the relative instability and aggregation inducing potential of bare and corona-bound BPEI-AuNP, over LA-and PEG-AuNP. Circular dichroism analysis showed surface chemistry-dependent conformational changes of proteins binding to AuNP. Timedependent uptake of bare, plasma corona (PC) and HSA corona-bound AuNP (HSA-AuNP) showed significant reduction in uptake with PC formation. Cell viability studies demonstrated dose-dependent toxicity of BPEI-AuNP. Transcriptional profiling studies revealed 126 genes, from 13 biological pathways, to be differentially regulated by 40 nm bare and PC-bound BPEI-AuNP (PC-BPEI-AuNP). Furthermore, PC formation relieved the toxicity of cationic BPEI-AuNP by modulating expression of genes involved in DNA damage and repair, heat shock response, mitochondrial energy metabolism, oxidative stress and antioxidant response, and ER stress and unfolded protein response cascades, which were aberrantly expressed in bare BPEI-AuNP-treated cells. NP surface chemistry is shown to play the dominant role over size in determining the biocorona composition, which in turn modulates cell uptake, and biological responses, consequently defining the potential safety and efficacy of nanoformulations. ARTICLE HISTORY

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“…22 Since the PC affects the properties of the NP surface, it has significant impact on the interaction between membrane and NPs. [23][24][25][26][27] Many studies are described in the literature where NP cellular uptake is related to the presence of a PC on the NPs in biological fluids, [28][29][30] in particular it has been shown that a key factor in NPs cellular uptake is their adhesion to the cell membrane that significantly affects their final uptake rates. 31 NP-cell membrane interaction is a complex dynamic process in which the environmental proteins are known to play an important multifaceted role: not only they adsorb onto the NP surface forming the PC, but also they compete with the NP in the interaction with the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio

Maccarini

Parker

et al. 2017

Journal of Colloid and Interface Science

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2 HypothesisIt is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. ExperimentsIn the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NPmembrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. FindingsOur study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon. KEYWORDSsupported lipid bilayer, protein corona nanoparticles, quartz crystal microbalance, neutron reflectometry, soft corona, hard corona. ABBREVIATIONNPs nanoparticles; SLB supported lipid bilayer; PC protein corona; QCM-D quartz crystal microbalance with dissipation; NR neutron reflectometry; PS polystyrene; PBS phosphate buffered saline; FBS fetal bovine serum; HC hard corona; SC soft corona; DOPC 1,2-Dioleoylsn-glycero-3-phosphocholine; SLD scattering length density; APM area per molecule; 4MW 4 matching water; SMW silicon matching water; LUV large unilamellar vesicle; GUV giant unilamellar vesicle; DPPC Dipalmitoyl-phosphatidyl-choline.3

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Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Cited by 256 publications

References 34 publications

Protein corona modulation of hepatocyte uptake and molecular mechanisms of gold nanoparticle toxicity

Protein corona modulation of hepatocyte uptake and molecular mechanisms of gold nanoparticle toxicity

Surface chemistry of gold nanoparticles determines the biocorona composition impacting cellular uptake, toxicity and gene expression profiles in human endothelial cells

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

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