The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio, Desirè Di; Maccarini, Marco; Parker, Roger; Mackie, Alan; Fragneto, Giovanna; Bombelli, Francesca Baldelli

doi:10.1016/j.jcis.2017.05.086

Cited by 45 publications

(41 citation statements)

References 60 publications

(76 reference statements)

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“…We realize that an intervening protein corona layer may also affect lipid corona formation. 33,43 Indeed, we have demonstrated in related experiments that the presence of certain proteins in certain nanoparticle coronas can coincide with the adsorption of nanoparticles to lipid bilayers that would otherwise not occur. 33 Future work will address the interplay between proteins and lipids in biomolecular coronas, including competitive exchange between the functional groups of nanoparticle coatings or wrappings and lipids versus proteins.…”

Section: Nanoparticle-vesicle Suspensions Form Aggregated Superstructmentioning

confidence: 94%

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Olenick

Troiano

Vartanian

et al. 2018

Chem

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Mechanisms of corona formation around nanomaterials remain enigmatic. Here, we provide evidence for spontaneous lipid corona formation that engenders new particle properties without the need for active mixing upon attachment to stationary and suspended lipid bilayer membranes. The mechanism of lipid corona formation can be used to improve control over nano-bio interactions and to help understand why some nanomaterial-ligand combinations are detrimental to organisms but others are not. SUMMARYAlthough mixing nanoparticles with certain biological molecules can result in coronas that afford some control over how engineered nanomaterials interact with living systems, corona formation mechanisms remain enigmatic. Here, we report results from experiments and computer simulations that provide concrete lines of evidence for spontaneous lipid corona formation without active mixing upon attachment to stationary and suspended lipid bilayer membranes. Experiments show that polycation-wrapped particles disrupt the tails of zwitterionic lipids, increase bilayer fluidity, and leave the membrane with reduced z potentials. Computer simulations suggest that the contact ion pairing between the lipid head groups and the polycations' ammonium groups leads to the formation of stable, albeit fragmented, lipid bilayer coronas. The mechanistic insight regarding lipid corona formation can be used to improve control over nanobio interactions and to help understand why some nanomaterial-ligand combinations are detrimental to organisms but others are not.

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Section: Nanoparticle-vesicle Suspensions Form Aggregated Superstructmentioning

confidence: 94%

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Olenick

Troiano

Vartanian

et al. 2018

Chem

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“…Experimentally, nanostructural studies performed with neutron reflectometry (NR) have focused on the mediating effect of the protein corona on the interaction between 20 and 100 nm diameter carboxylated polystyrene nanoparticles and solid supported lipid bilayer (SLB), and on the effect of the lipid composition on the interaction between 10 nm super paramagnetic iron oxide nanoparticles and SLB . However, the strong interaction between the bilayer and the supporting substrate can have an unpredictable impact on the membrane properties.…”

Section: Introductionmentioning

confidence: 99%

The Role of Temperature and Lipid Charge on Intake/Uptake of Cationic Gold Nanoparticles into Lipid Bilayers

Lolicato

Joly

Martinez‐Seara

et al. 2019

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Understanding the molecular mechanisms governing nanoparticlemembrane interactions is of prime importance for drug delivery and biomedical applications. Neutron reflectometry (NR) experiments are combined with atomistic and coarse-grained molecular dynamics (MD) simulations to study the interaction between cationic gold nanoparticles (AuNPs) and model lipid membranes composed of a mixture of zwitterionic di-stearoyl-phosphatidylcholine (DSPC) and anionic di-stearoylphosphatidylglycerol (DSPG). MD simulations show that the interaction between AuNPs and a pure DSPC lipid bilayer is modulated by a free energy barrier. This can be overcome by increasing temperature, which promotes an irreversible AuNP incorporation into the lipid bilayer. NR experiments confirm the encapsulation of the AuNPs within the lipid bilayer at temperaturesaround 55 °C. In contrast, the AuNP adsorption is weak and impaired by heating for a DSPC-DSPG (3:1) lipid bilayer. These results demonstrate that both the lipid charge and the temperature play pivotal roles in AuNPmembrane interactions. Furthermore, NR experiments indicate that the (negative) DSPG lipids are associated with lipid extraction upon AuNP adsorption, which is confirmed by coarse-grained MD simulations as a lipid-crawling effect driving further AuNP aggregation. Overall, the obtained detailed molecular view of the interaction mechanisms sheds light on AuNP incorporation and membrane destabilization.

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“…In this context, supported lipid bilayers (SLBs), are particularly relevant as they provide a stable flat structure and a highly tuneable composition, which make them optimal candidates for many different kinds of experiments involving surface-sensitive techniques [18][19][20]. Due to their extensive implementation, detailed structural descriptions of SLBs are fundamental for the investigation of more complex systems, as those involving lipids and proteins [21][22][23].…”

Section: Introductionmentioning

confidence: 99%