In vitro hemolysis of human erythrocytes — by plant extracts with antiplasmodial activity

Sharma, Poonam; Sharma, Jayashri Devi

doi:10.1016/s0378-8741(00)00370-6

Cited by 157 publications

(94 citation statements)

References 10 publications

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“…A avaliação da estabilidade mecânica da membrana eritrocitária é um bom indicador de danos provocados por vários compostos como triagem de citotoxicidade. 39 A cultura de células fornece uma importante ferramenta para estudo da citotoxicidade de compostos com potencial atividade terapêutica 40 definindo o potencial de degeneração ou morte celular provocado pelo material constituinte nos cosméticos. Assim, resultados positivos no ensaio de citotoxicidade descaracterizam a condição de inocuidade do cosmético, sendo possível causar processos de irritabilidade nos usuários.…”

Section: Resultsunclassified

Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Yamaguchi¹,

Veiga²,

Pedrosa³

et al. 2013

Quím. Nova

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Recebido em 3/10/12; aceito em 29/1/13; publicado na web em 21/3/13 BIOLOGICAL ACTIVITIES OF ESSENTIAL OILS OF Endlicheria citriodora, A METHYL GERANATE-RICH LAURACEAE. The essential oils of branches and leaves of Endlicheria citiodora were obtained by hydrodistillation and analysed using GC-FID, GC-MS and both NMR 13 C and 1 H, resulting in the identification of methyl geranate as major constituent (93%) in both oils. Cytotoxicity, tyrosinase-inhibition and antioxidant activities were studied and characterized. High antioxidant potential (15.52 and 13.53 µg/mL), low cytotoxicity and tyrosinase inhibition (53.85%) were observed. This is the first paper reporting the biological activities and composition of the essential oils of this species.Keywords: linalool; tirosinase; Amazon. INTRODUÇÃOLauraceae é uma família botânica conhecida por possuir espécies com interesse comercial pelos seus óleos essenciais, com aproximadamente 50 gêneros e 2500 espécies. Destas, 400 espécies distribuídas em 25 gêneros são encontradas no Brasil, possuindo grande incidência na região Amazônica. [1][2][3] Os estudos já publicados descrevem a composição dos óleos essenciais de Lauraceae com predominância de terpenos. Este é o caso de Aniba rosaeodora, A. duckei e Cinnamomum camphora e outras espécies cujos constituintes majoritários são monoterpenos e hidrocarbonetos sesquiterpênicos, encontrados em espécies de diversos gêneros. [4][5][6][7][8][9][10][11] Fenilpropanóides, como safrol, metileugenol, derivados de cinamaldeído e benzenóides 6,12-14 são encontrados em espécies de Ocotea, como O. odorifera, O. pretiosa e O. sassafras. Mesmo apresentando elevado potencial aromático em suas espécies, há muitos gêneros que não possuem descrição de seus óleos essenciais, como é o caso de Endlicheria, que tem cerca de 60 espé-cies distribuídas na América Tropical. 3 Nos estudos envolvendo as espécies desse gênero só há relatos da composição de extratos, como o isolamento de neolignanas hexahidrobenzofuranóides (disodantina A, disodantina B, acetato de megafone, burchelina) e derivados bioativos de benzoato de benzila (2-hidróxibenzoato de benzila; 2-hidróxi-6-metóxibenzoato de benzila; 2,6-dimetóxibenzoato de benzila e 2,5-dimetóxibenzoato de benzila). 15,16 O uso tradicional das plantas contra doenças de pele, especialmente para fins cosméticos, é uma prática comum na medicina popular de muitas culturas e pode fornecer pistas para a descoberta de melhores agentes despigmentantes. 17,18 Formulações cosméticas produzidas a partir de ativos botânicos são utilizadas desde a antiguidade e extratos vegetais continuam desempenhando importante papel nos cosméticos contemporâneos. 19 A inibição da tirosinase, enzima chave na melanogênese, é o principal mecanismo de ação de agentes despigmentantes como o ácido kójico. Assim, os pesquisadores têm se baseado nessa atividade na busca por novos agentes clareadores. 20 Considerando o interesse das espécies da família Lauraceae, o presente trabalho teve como objetivo descrever a composição química dos óleos...

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Section: Resultsunclassified

Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Yamaguchi¹,

Veiga²,

Pedrosa³

et al. 2013

Quím. Nova

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“…Studies indicate that some plant substances like polyphenols, epicatechins, steryl glycosides and triterpenoid saponins cause damage to red cell membranes and produce hemolysis (Costa-Lotufo et al, 2002). The mechanical stability of erythrocyte membrane a good indicator of insults by vegetal substances (Sharma and Sharma, 2001;Santos et al, 2010). Then, hemolysis detection is an useful and cheap technique which can displays the effect of increasing concentrations and can to be sigmoidally related to the logarithm contact time, emphasizing the membrane stability as a biological complex to maintain its structure under stress conditions, such as oxidation, hipotonicity, pH changes, heat and in presence of osmotic active solutes (Van Ginkel and Sevanian, 1994;Sharma and Sharma, 2001;Freitas et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The mechanical stability of erythrocyte membrane a good indicator of insults by vegetal substances (Sharma and Sharma, 2001;Santos et al, 2010). Then, hemolysis detection is an useful and cheap technique which can displays the effect of increasing concentrations and can to be sigmoidally related to the logarithm contact time, emphasizing the membrane stability as a biological complex to maintain its structure under stress conditions, such as oxidation, hipotonicity, pH changes, heat and in presence of osmotic active solutes (Van Ginkel and Sevanian, 1994;Sharma and Sharma, 2001;Freitas et al, 2008). Herein, none of the extracts tested caused hemolysis even at the highest concentration (200 μg/ml), suggesting that the mechanism of cytotoxicity is probably related to a more specific pathway.…”

Section: Discussionmentioning

confidence: 99%

Biological screening of extracts of Brazilian Asteraceae plants

Carvalho¹,

Machado²,

Ferreira³

et al. 2013

Afr. J. Pharm. Pharmacol.

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Natural products are a very productive source of leads for the development of medicines. Seven Brazilian Asteraceae adult plants were randomly chosen. The current study was designed to evaluate the antiprotozoal and cytotoxic activities in vitro of 21 extracts obtained. Phytochemical properties of the most active extracts also were checked. Cytotoxic activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines (HCT-116, OVCAR 8 and SF-295). The antiprotozoal activity was evaluated against Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. None of the extracts showed antiprotozoal activity. However, 76% of the extracts displayed moderate to high in vitro cytotoxic activities against human cancer cell which suggests that they are a promising source of anticancer compound, since none of the extracts showed hemolytic activity. Terpenoids, flavonoids, saponins, tannins, besides other compound classes, were identified and may be responsible for their antitumor activity. Cytotoxic assays indicate the anticancer potential of Asteraceae species from Brazil.

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“…The toxic effect of C. swynnertonii at higher doses (for example 1000 mg) could be linked to hemolytic effect of resin (Bakari et al, 2013) which in turn, could increase total bilirubin (Sharma and Sharma, 2001).…”

Section: Effect Of C Swynnertonii Resin On Liver Functionmentioning

confidence: 99%

Effect of Commiphora swynnertonii resin on liver functions and levels of plasma glucose and lipids in carbon-tetrachloride treated chickens

Maghembe¹,

Bakari²,

Max³

et al. 2017

Int. J. Nutr. Metab.

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A 21-day study was carried out to determine the effect of resin from Commiphora swynnertonii on plasma levels of glucose and lipids together with liver functions in both normal and carbontetrachloride-treated chickens. Sixty growing male broiler chickens were randomly assigned into six groups (n = 10). G1 was a negative control, which received distilled water throughout the experiment. G2 was a positive control and received 1.59 g CCl 4 per kg body weight orally every other day to induce liver injury. G3 received 500 mg resin per kg body weight daily, while G4, G5 and G6 received 500, 750 and 1000 mg resin per kg body weight respectively daily plus 1.59 g CCl 4 per kg body weight every other day, throughout the experiment. Plasma levels of glucose, triglycerides, lipoproteins and total proteins together with liver functions were determined. Results revealed that oral administration of CCl 4 caused liver injury with consequent significant increase (P < 0.05) in the plasma glucose and lipid levels. Also a significant increase (P < 0.05) in the activities of liver enzymes (P< 0.01) and a decrease in total plasma protein was evident. On the other hand, oral administration of moderate dose of the resin reversed these effects by significantly lowering plasma glucose and lipid levels while decreasing liver enzyme activities and boosting plasma protein levels. It is concluded that administration of moderate levels of C. swynnertonii to chickens was capable of reducing plasma levels of glucose (hypoglycemic effect) and lipids (hypolipidemic effect), while protecting the liver against the injury caused by CCl 4 (hepatoprotective effect). These findings could have a positive implication on the use of the C. swynnertonii resin for treating diabetes and cardiovascular diseases in humans.

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In vitro hemolysis of human erythrocytes — by plant extracts with antiplasmodial activity

Cited by 157 publications

References 10 publications

Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

Biological screening of extracts of Brazilian Asteraceae plants

Effect of Commiphora swynnertonii resin on liver functions and levels of plasma glucose and lipids in carbon-tetrachloride treated chickens

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