IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT

Abstract

The development of micropropagule and embryo of seaweed depend on nutrient and fertilizer used. Seaweed has been reported contain hormone regulators such as auxine, cytokinine, gibbereline, and various minerals applied in stimulating the growth ocra plant and wheat culture. The objectives of this study were to determine the potential of Kappaphycus alvarezii extract and its optimal concentration in accelerating of Kappaphycus alvarezii micropropagule and embryo growth. Micropropagule and embryo produced through callus induction were planted into PES 1/20 liquid medium supplemented with seaweed extract at the concentrations of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed that medium enrichment with 50 μL of seaweed extract had the highest survival rate and growth of thallus. In addition, this concentration was also resulted in a good performance of K. alvarezii thallus with the lighter color. The advantage of this study for seaweed cultivation in Indonesia, among others, seaweed can be used as fertilizer, especially in the maintenance of seaweed seed, so that cultivation can be better develop.

Penambahan Sari Akar Eceng Gondok (Eichornia crassipes) Media MS pada Pertumbuhan Tahap Subkultur I Anggrek Bulan (Phalaenopsis sp.)

“…Berdasarkan Gambar 1 di atas menunjukkan bahwa berbeda nyata setiap perlakuan. Hal ini sesuai dengan pendapat Suryati et al (2015), menyatakan bahwa penambahan zat pengatur tumbuh seperti auksin, giberelin, dan sitokinin ataupun ekstrak organik lainnya masih dibutuhkan dalam penggunaan media dasar. Unsur hara mikro dan makro yang berperan dalam pembentukan klorofil, memperlancar proses fotosintetis, dan enzim proses sintesis protein (Taufiq, 2014).…”

Komponen Sargassum Aquifolium Sebagai Hormon Pemicu Tumbuh Untuk Eucheuma Cottonii

“…Bagian utama tumbuhan didukung oleh konsentrasi tinggi sitokinin, sedangkan perakaran dipromosikan oleh konsentrasi auksin yang tinggi. Suryati, E. et al, 2015. Menjelaskan kehadiran hormon endogen seperti auksin, sitokinin, dan asam absisat pada 11 spesies ganggang merah dari pantai Brazil. Auksin dan sitokinin memiliki peran regulasi dalam pertumbuhan dan morfogenesis in vitro pada beberapa spesies alga merah, seperti Gracilaria tenuistipitata, Gracilaria perplexa, dan Solieria filiformis.…”