In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries

Hasegawa, Akiko; Hamada, Yukari; Mehandjiev, Tzvetozar; Koyama, Koji

doi:10.1016/j.fertnstert.2003.08.028

Cited by 45 publications

(33 citation statements)

References 38 publications

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“…Vitrification is a relatively inexpensive procedure and does not require any special instrument [10]. Ovarian tissue vitrification has been performed in mice [11][12][13], domestic animals [14][15][16][17], nonhuman primates [18][19][20], and in humans [21][22][23][24]. To date, no pregnancy has been reported in humans after transplantation of vitrified tissue [3].…”

Section: Introductionmentioning

confidence: 99%

In vitro development of human primordial follicles to preantral stage after vitrification

Khosravi

Reid

Moini

et al. 2013

J Assist Reprod Genet

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Purpose The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue. Methods Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed. Results High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P <0.05) whereas primordial and transitory follicles showed a significant decrease (P <0.05) compared to their respective counterparts at day 0 of culture.Conclusions Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.

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Section: Introductionmentioning

confidence: 99%

In vitro development of human primordial follicles to preantral stage after vitrification

Khosravi

Reid

Moini

et al. 2013

J Assist Reprod Genet

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“…This procedure decreases or totally eliminates the risk of transmitting malignant cells from cryopreserved ovarian tissue back into recipients and also allows direct monitoring and assessment of follicle quality during the maturation period (Amorim et al 2009). Live pups have been obtained from culture of isolated pre-antral follicles from cryopreserved fetal, newborn, and immature mice (Cox et al 1996, Liu et al 2001, Migishima et al 2003, Hasegawa et al 2004; however, there has been no attempt to achieve pregnancies from in vitro culture of pre-antral follicles from adult animals.…”

Section: Introductionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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“…Until now, vitrification has been adopted to cryopreserve embryos [3,4], oocytes [5][6][7][8][9] and ovarian tissue [10][11][12][13]. Kuwayama reported that vitrification, compared with slow cooling, resulted in high survival rates for all stages of embryo development [14].…”

Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

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In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries

Cited by 45 publications

References 38 publications

In vitro development of human primordial follicles to preantral stage after vitrification

In vitro development of human primordial follicles to preantral stage after vitrification

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

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