Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Abstract

Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

Heterotopic ovarian transplantation results in less apoptosis than orthotopic transplantation in a minipig model

“…We found a superior response in heterotopic implants compared with orthotopic implants, including the observation that the heterotopic cryopreserved implants lost fewer follicles in comparison to orthotopic implants. Our findings are consistent with those of other reports [ 1 ] and suggest that fresh and subcutaneous transplants exhibit a potential that has thus far not been effectively utilized in research [ 8 , 27 , 28 ].…”

Assessment of the effect of different vitrification solutions on human ovarian tissue after short‐term xenotransplantation onto the chick embryo chorioallantoic membrane

“…A clear understanding of the events that occur during the early stages of ovarian tissue transplantation will therefore reduce injury to both the follicles and stromal cells. Previous studies have tracked the successful xenotransplantation of ovarian tissue into severe‐combined immunodeficiency disease (SCID) mice (Zhang et al, ; Amorim et al, ; Dittrich et al, ; Jafarabadi et al, ); here, we grafted thawed ovarian tissue onto a chick embryo chorioallantoic membrane (CAM) model for a short time to track the early events following transplantation.…”

Adding Ascorbic Acid to Vitrification and IVC Medium Influences Preantral Follicle Morphology, but Not Viability

“…sucrose, enhances cell dehydration before freezing of extracellular water. It has been stated that sucrose concentrations ranging from 0.4 to 0.8 m are appropriate to vitrify mouse ovaries (Zhang et al. 2009).…”

“…sucrose, enhances cell dehydration before freezing of extracellular water. It has been stated that sucrose concentrations ranging from 0.4 to 0.8 M are appropriate to vitrify mouse ovaries (Zhang et al 2009). In a previous study, we have shown the suitability of 0.5 M sucrose in the vitrification of caprine ovarian tissue (Santos et al 2007a).…”