Assessment of the effect of different vitrification solutions on human ovarian tissue after short‐term xenotransplantation onto the chick embryo chorioallantoic membrane

Zeng, Yucui; Tang, Huiru; Zeng, Liping; Chen, Yun; Wang, Guoping; Wu, Ruifang

doi:10.1002/mrd.22631

Cited by 6 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preparation of the chick embryo chorioallantoic membrane (CAM) for transplantation of cancer cells were performed as described early [15,16]. Briefly, fertilized eggs of White Leghorn chickens were purchased at a local hatchery and incubated at 37°C-38°C with 60% relative humidity for 3 days.…”

Section: Cam-xenotransplantation: Induction Of Angiogenesis and Tumormentioning

confidence: 99%

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation

Todorov

Isachenko

et al. 2020

BMC Cancer

View full text Add to dashboard Cite

Background: Ovarian tissue cryopreservation has a wide range of cancerous indications. Avoiding relapse becomes a specific concern that clinicians frequently encounter. The data about the comparative viability of cancer cells after cryopreservation are limited. This study aimed to evaluate the effect of cryopreservation on breast cancer cells. Methods: We used in-vitro cultured ZR-75-1 and MDA-MB-231 cell lines. Cell samples of each lineage were distributed into the non-intervened and cryopreserved groups. The cryopreservation procedures comprised programmed slow freezing followed by thawing at 100°C, 60 s. Biological phenotypes and the related protein markers were compared between the two groups. The EVOS FL Auto 2 Cell Image System was used to monitor cell morphology. Cell proliferation, motility, and penetration were characterized by CCK-8, wound-healing, and transmembrane assay, respectively. The expression of Ki-67, P53, GATA3, E-cadherin, Vimentin, and F-Actin was captured by immunofluorescent staining and western blotting as the proxy measurements of the related properties. The chorioallantoic membrane (CAM) xenotransplantation was conducted to explore angiogenesis induced by cancer cells. Results: After 5 days in vitro culture, the cell concentration of cryopreserved and non-intervened groups was 15.7 × 10 4 vs. 14.4 × 10 4 cells/ml, (ZR-75-1, p > 0.05), and 25.1 × 10 4 vs. 26.6 × 10 4 cells/ml (MDA-MB-231, p > 0.05). Some cryopreserved ZR-75-1 cells presented spindle shape with filopodia and lamellipodia and dissociated from the cell cluster after cryopreservation. Both cell lines demonstrated increased cell migrating capability and invasion after cryopreservation. The expression of Ki-67 and P53 did not differ between the cryopreserved and non-intervened groups. E-cadherin and GATA3 expression downregulated in the cryopreserved ZR-75-1 cells. Vimentin and F-actin exhibited an upregulated level in cryopreserved ZR-75-1 and MDA-MB-231 cells. The cryopreserved MDA-MB-231 cells induced significant angiogenesis around the grafts on CAM with the vascular density 0.313 ± 0.03 and 0.342 ± 0.04, compared with that of non-intervened cells of 0.238 ± 0.05 and 0.244 ± 0.03, p < 0.0001.

show abstract

Section: Cam-xenotransplantation: Induction Of Angiogenesis and Tumormentioning

confidence: 99%

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation

Todorov

Isachenko

et al. 2020

BMC Cancer

View full text Add to dashboard Cite

show abstract

“…Preparation of the chick embryo chorioallantoic membrane (CAM) for transplantation of cancer cells were performed as described early [15,16]. Brie y, fertilized eggs of White Leghorn chickens were purchased at a local hatchery and incubated at 37 °C-38 °C with 60% relative humidity for three days.…”

Section: Model Of Solid Tumors Tissue Cam-xenotransplantation: Stimulmentioning

confidence: 99%

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-Xenotransplantation.

Todorov²,

Isachenko

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Ovarian tissue cryopreservation has a wide range of cancerous indications. Avoiding relapse becomes a specific area of concern that clinicians frequently encounter. The data about the comparative viability of cancer cells after cryopreservation are limited. This study aimed to evaluate the effect of cryopreservation on breast cancer cells.Methods: Samples were prepared using ZR-75-1 and MDA-MB-231 cell lines and divided into cryopreserved and non-intervened groups, respectively. Biological properties and the related protein markers were investigated. Cell morphology was monitored under the microscope. Cell proliferation, migration, and invasion were characterized by CCK-8, wound-healing, and transmembrane assay, respectively. The expression of Ki-67, P53, GATA-3, E-cadherin, Vimentin, and F-Actin was measured by immunofluorescent staining and western blotting. Xenotransplantation was established on the chorioallantoic membrane (CAM) culture system to explore angiogenesis close to the grafts.Results: Lamellipodia and filopodia were observed in cryopreserved ZR-75-1 cells. Both cell lines demonstrated increased cell motility and invasive ability after cryopreservation. However, cell proliferation was invariable in accordance with a regular expression of Ki-67 and P53. In ZR-75-1 cells, data exhibited a downregulation of E-cadherin after the decreased expression of GATA3, indicating the loss of intercellular adhesion after cryopreservation. Vimentin and F-actin were both upregulated in cryopreserved sample cells. Angiogenesis in CAM was significantly activated by cryopreserved MDA-MB-231 cells.Conclusions: Cryopreservation causes an increasing malignancy of ZR-75-1 and MDA-MB-231 cells and thus raises the risk of metastasis.

show abstract

Review of non-permeating cryoprotectants as supplements for vitrification of mammalian tissues

et al. 2020

View full text Add to dashboard Cite

Assessment of the effect of different vitrification solutions on human ovarian tissue after short‐term xenotransplantation onto the chick embryo chorioallantoic membrane

Cited by 6 publications

References 54 publications

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-Xenotransplantation.

Review of non-permeating cryoprotectants as supplements for vitrification of mammalian tissues

Contact Info

Product

Resources

About