In vitro development of human primordial follicles to preantral stage after vitrification

Khosravi, Fariba; Reid, Robert L.; Moini, Ashraf; Abolhassani, Farid; Valojerdi, Mojtaba Rezazadeh; Kan, Frederick W. K.

doi:10.1007/s10815-013-0105-z

Cited by 19 publications

(24 citation statements)

References 65 publications

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“…Usually, a short period of 2 days is not enough to assess changes in the follicular density of follicular growth [38,39]. However, the growth of follicles during the 7-day culture that we observed was in accordance with previous studies [40].…”

Section: Discussionsupporting

confidence: 91%

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model

Tanpradit

Chatdarong

Comizzoli

2016

J Assist Reprod Genet

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Purpose Temporary and reversible downregulation of metabolism may improve the survival of tissues exposed to nonphysiological conditions during transport, in vitro culture, and cryopreservation. The objectives of the study were to (1) optimize the concentration and duration of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP-a mitochondrial uncoupling agent) exposures for biopsies of domestic cat ovarian tissue and (2) examine the effects of FCCP preexposures on follicle integrity after tissue culture and/or cryopreservation. Methods Biopsies of cat ovarian tissue were first treated with various concentrations of FCCP (0, 10, 40, or 200 nM) for 10 or 120 min to determine the most suitable pre-exposure conditions. Based on these results, tissues were pre-exposed to 200 nM FCCP for 120 min for the subsequent studies on culture and cryopreservation. In all experiments and for each treatment group, tissue activity and integrity were measured by mitochondrial membrane potential (relative optical density of rhodamine 123 fluorescence), follicular viability (calcein assay), follicular morphology (histology), granulosa cell proliferation (Ki-67 immunostaining), and follicular density.Results Ovarian tissues incubated with 200 nM FCCP for 120 min led to the lowest mitochondrial activity (1.17 ± 0.09; P < 0.05) compared to control group (0 nM; 1.30 ± 0.12) while maintaining a constant percentage of viable follicles (75.3 ± 7.8 %) similar to the control group (71.8 ± 11.7 %; P > 0.05). After 2 days of in vitro culture, percentage of viable follicles (78.8 ± 8.9 %) in similar pre-exposure conditions was higher (P < 0.05) than in the absence of FCCP (61.2 ± 12.0 %) with percentages of morphologically normal follicles (57.6 ± 17.3 %) not different from the fresh tissue (70.2 ± 7.1 %; P > 0.05). Interestingly, percentages of cellular proliferation and follicular density were unaltered by the FCCP exposures. Based on the indicators mentioned above, the FCCP-treated tissue fragments did not have a better follicle integrity after freezing and thawing. Conclusions Pre-exposure to 200 nM FCCP during 120 min protects and enhances the follicle integrity in cat ovarian tissue during short-term in vitro culture. However, FCCP does not appear to exert a beneficial or detrimental effect during ovarian tissue cryopreservation.

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Section: Discussionsupporting

confidence: 91%

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model

Tanpradit

Chatdarong

Comizzoli

2016

J Assist Reprod Genet

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“…In vitro culture of ovarian follicle is a valuable bioengineering technique for investigating the factors that control oocyte development at different developmental stages and as an alternative therapy for fertility preservation (Khosravi et al , ; Kim et al , ). However, currently available in vitro follicle culture techniques are labour‐intensive and of low efficiency, as most investigators collect and seed single pre‐antral follicles for monofollicular maturation.…”

Section: Introductionmentioning

confidence: 99%

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

Kim

Kang

et al. 2016

J Tissue Eng Regen Med

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In vitro culture of ovarian follicles is a promising bioengineering technique for retrieving fertilizable oocytes from preserved ovarian tissues of cancer survivors. However, current in vitro follicle culture techniques are labour-intensive and of low efficiency, as only single follicle culture (SFC) has been possible to date. The present study investigated the feasibility of multifollicular cluster culture (MFCC) system using angiotensin II receptor (ATII-Rc) analogues. Ovarian pre-antral follicles isolated from 2-week-old C57BL6 mice were cultured with ATII-Rc agonist or antagonist and their maturation outcomes were compared with control group. When single follicles were cultured, the ovulation and maturation rates were similar in all three groups. When three-follicle clusters were cultured, up to three follicles were ovulated in the ATII-Rc agonist group while none or one follicle ovulated in control or antagonist groups (p < 0.0001). Significantly higher numbers of mature oocytes were obtained in the agonist group (three-follicle 28.2 ± 4.9 vs. SFC 11.0 ± 1.3, per 25 cultured droplets) (p < 0.0001), and the development of each fertilized oocytes was comparable to those from SFC. It is therefore concluded that this novel MFCC system can significantly improve the efficiency of in vitro mature oocyte retrieval via ATII-Rc modulation. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Vitrification of ovarian tissue is a promising approach with great potential to preserve fertility (Donnez et al. ; Donnez et al, ; Khosravi et al., ) of non‐human animals; however, few data are available, especially for canids. To our knowledge, this study was the first to assess follicular density and histological features from fresh and vitrified–warmed ovarian cortex from domestic dogs.…”

Section: Discussionmentioning

confidence: 99%

“…Isolated follicles in a 3D matrix have been cultured in vitro in an attempt to obtain antral follicles. Good results were obtained for primates (Khosravi et al., ; Xu et al., ) and rats (Wang et al., ); however, follicle isolation in dogs has been proven to be laborious and with a low rate of success (Ackermann et al. ).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of follicular growth and tissue viability in vitrified/warmed domestic dog ovaries after in vitro culture

Ackermann

Krisher

et al. 2016

Reprod Domestic Animals

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ContentsCryopreservation of gametes is an important tool to preserve fertility, but for most species, including domestic dogs, data regarding ovarian tissue cryopreservation are limited. We aimed to evaluate the follicular and tissue viability and follicular growth after in vitro culture of domestic dog ovarian cortical slices cryopreserved by vitrification. Ovarian cortex was obtained from ten pairs of ovaries from domestic dogs using two methods (A and B), one for each ovary from the same bitch. At least four slices for each method were obtained from each ovary, one was processed for histology and the other three were vitrified. When the vitrified slices were warmed, one slice from each method was processed for histology and the remaining two slices were cultured in vitro for 7 days, after which they were processed for histological evaluation. Density of follicles in fresh samples was similar for both methods. For Method A, density of secondary follicles decreased, while the density of primordial follicles was maintained throughout the process. For Method B, density of primary follicles decreased after 7 days of incubation, but density of secondary follicles increased, confirming follicular growth in Method B. Overall, there were no differences between Methods A and B in follicular integrity after incubation. Fresh samples showed better arterial, venous and follicle preservation, followed by vitrified-warmed samples, but no differences were observed between methods. In conclusion, the methodology used to isolate the ovarian cortex may affect tissue and follicle viability as well as follicular development during in vitro culture.

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In vitro development of human primordial follicles to preantral stage after vitrification

Cited by 19 publications

References 65 publications

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

Evaluation of follicular growth and tissue viability in vitrified/warmed domestic dog ovaries after in vitro culture

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