In vitro flowering and production of viable pollen of cucumber

Kiełkowska, Agnieszka; Havey, Michael J.

doi:10.1007/s11240-011-0075-8

Cited by 21 publications

(13 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The formation of seeds requires flowering phase in such commercial oil crops. Therefore, in vitro propagation along with in vitro flowering will not only help in production of better quality of oil plants but will also ensure shortening of the breeding cycle (Sivanesan and Jeong 2007;Kielkowska and Havey 2012;Haque and Ghosh 2013a) and studying the developmental pattern of flowers from vegetative to floral phase (Huang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Baghel

Bansal

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl(-1)). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl(-1)) + GA3 (0.1 mgl(-1)). The plantlets thus formed were successfully hardened with 90 % survival.

show abstract

Section: Introductionmentioning

confidence: 99%

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Baghel

Bansal

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Also, flowering was observed during the acclimatization phase (Figure 1h), an interesting aspect since early flowering of micropropagated plantlets can shorten the breeding cycle to meet the market demands (Kiełkowska & Havey, 2012).…”

Section: Acclimatizationmentioning

confidence: 99%

A Rapid and Efficient Protocol for Clonal Propagation of Phenolic-Rich Lavandula multifida

Zuzarte¹,

Dinis²,

Salgueiro³

et al. 2015

JAS

View full text Add to dashboard Cite

Lavandula multifida (fernleaf lavender) grows in confined regions of Portugal. This species is known for its interesting chemical profile, rich in phenolic compounds, and significant antifungal and anti-inflammatory properties, making it an interesting plant for industrial exploitation and justifying alternative propagation methods. Therefore, the present study aims the development of an efficient protocol for large scale propagation of this species, assuring plant uniformity and aiming a sustainable industrial exploitation with preservation of natural resources.In vitro cultures were established on Murashige and Skoog medium (MS) with different concentrations of benzyladenine (BA) and zeatin (ZEA). Best multiplication rates (6.14 shoots per explant) were achieved using MS supplemented with 1.0 mg/L ZEA and rooting occurred spontaneously on the propagation medium avoiding additional rooting steps. Moreover, plants flowered during the acclimatization phase, confirming a more rapid in vitro development and shorter breeding cycle. This is a very interesting aspect for essential oil extraction. Trichome morphology was examined by scanning electron microscopy and the secretion analyzed through histochemical tests. Trichomes of micropropagated plantlets were identical to those of field-growing plants, and essential oil production was observed at early stages of development.The present study confirms that micropropagation under controlled conditions could be an interesting alternative for field production of L. multifida, since higher amounts of plants can be obtained in less time with guarantee of genetic stability and phytossanitary quality, very important features regarding essential oil production and commercialization.

show abstract

“…The culture medium was 1.0 % sucrose supplemented with 1.0 % boric acid, 5 mg L -1 GA 3 and 1.0 % agar. The hanging drop method was then applied in experiments, with exception of those where sugar agar constituted the culture medium base (Kielkowska and Havey 2012). Pollen vigor was tested by the TTC (2,3,5-Triphenyltetrazolium chloride) staining method, whereby pollen was placed into a 1 % TTC solution for 30 min for microscopic examination (Khoddamzadeh et al 2011).…”

Section: Southern Blotting and Northern Blottingmentioning

confidence: 99%

Inducing male sterility of tomato using two component system

Cao

Wei

Liu

et al. 2012

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Production of hybrid seeds and pursuing heterosis breeding of many crops have been accomplished using male sterile lines. However, not all crops have valuable male sterile lines due to instability of male sterility and absence of a restorer system. In this study, male sterile lines have been induced using a two-component system. The extracellular ribonuclease Barnase was cleaves into two inactive yet complementary fragments, designated as 'Bn-5' and 'Bn-3'. Both components were controlled by a TA29 promoter. They were transferred into the tomato inbred line 'Yellow tomato' by Agrobacterium method. Southern blotting identified that 11 transgenic Bn-5 plants (T 0 ) and 10 transgenic Bn-3 plants (T 0 ) were obtained. The vegetative phenotypes of all T 0 plants were similar to wild-type, and they were capable of producing viable pollen grains and normal fruit with seeds, indicating that Barnase had lost its function after it being split two partial fragments. After self-pollination, homozygous progenies (T 1 ) of transgenic Bn-5 and Bn-3 plants were chosen to cross each other, Barnase could be reconstituted and co-expressed in the same cell, which caused the hybrid plants to produce collapsed pollen grains with no viability and thus100 % male sterile plants were obtained. Stamens of male sterile plants were shorter than those of the wild type plants. PCR detection demonstrated that all male sterile plants contained Barnase, but male fertile plants did not. The male sterile plants were crossed with the male fertile inbred lines, and the result showed that hybrid (F 1 ) plants were capable of producing normal fruit with seeds, and their pollen grain fertility was restored. The co-segregation ratio of Bn-5 and Bn-3 fragments showed 1:1 among hybrid plants. In conclusion, the results verified that the male sterility could be generated by two component system and be used in hybrid seed production. The F 1 between the male sterile plant and the inbred line showed heterotic comparing to both parents. This system needs not breed restoration line.

show abstract

In vitro flowering and production of viable pollen of cucumber

Cited by 21 publications

References 41 publications

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

A Rapid and Efficient Protocol for Clonal Propagation of Phenolic-Rich Lavandula multifida

Inducing male sterility of tomato using two component system

Contact Info

Product

Resources

About