In vitro feeding of Dermanyssus gallinae

Kirkwood, A.C.

doi:10.1016/0014-4894(71)90002-6

Cited by 24 publications

(14 citation statements)

References 18 publications

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“…Similar results were obtained by Voigt et al (1993) with the tick Amblyomna variegatum (L.) (Acari: Ixodidae) and it was suggested that EDTA might interfere with Ca + ‐dependent mechanisms. The best feeding and survival rates for artificially fed parasitic mites and ticks were obtained with heparin, a protein that is naturally present in blood (Kirkwood, 1971; Voigt et al , 1993; Bruneau et al , 2001).…”

Section: Discussionmentioning

confidence: 99%

Effects of blood type and blood handling on feeding success, longevity and egg production in the body louse, Pediculus humanus humanus

Mumcuoglu

Danilevich

Zelig

et al. 2011

Medical and Veterinary Entomology

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The effects of feeding different types of human blood to human body lice, Pediculus humanus humanus L. (Phthiraptera: Pediculidae), on feeding success, longevity and numbers of eggs laid were investigated using an artificial blood-feeding system in the laboratory. No significant differences were found between lice fed on different human blood types for any of the parameters tested. However, when lice were fed on human blood of one blood type followed immediately by a different blood type, they took significantly smaller bloodmeals, their longevity was reduced and they laid fewer eggs per female than control lice that had been fed twice on the same human blood type. When lice were fed human blood that had been stored for 1-26 weeks, the quantity of blood taken, the proportion of lice that became fully engorged and lice longevity diminished gradually as the storage time of the blood increased, but there was no effect of storage time on the mean number of eggs laid per female. However, lice would not feed on 26-week-old blood. The type of anticoagulant used had a significant effect on the proportion fed, longevity and number of eggs laid per female. Generally, EDTA (ethylenediaminetetraacetic acid)-treated blood reduced longevity and the number of eggs laid per female to a greater degree than heparinized or citrated blood. Lice fed on rabbit blood took significantly larger amounts of blood, lived longer and laid a higher mean number of eggs per female than lice fed on human blood.

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Section: Discussionmentioning

confidence: 99%

Effects of blood type and blood handling on feeding success, longevity and egg production in the body louse, Pediculus humanus humanus

Mumcuoglu

Danilevich

Zelig

et al. 2011

Medical and Veterinary Entomology

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“…Table 3 shows that 86.9% of mites fed on citrated blood, that is, as well as on heparinized blood in other tests (av. However, unlike northern fowl mites (Table 3), many fewer Dermanyssus gallinae (DeGeer) fed on heparinized blood (18.0~ than citrated blood (70.307o) (Kirkwood, 1971). On the other hand, fewer mites fed on oxalated blood and survived (66.1%), and these mites were all moribund.…”

Section: Bloodmentioning

confidence: 96%

“…Furthermore, 33 % of these oxalate-fed mites but only 22% of citrate-fed mites were dead by the 12th day. However, unlike northern fowl mites (Table 3), many fewer Dermanyssus gallinae (DeGeer) fed on heparinized blood (18.0~ than citrated blood (70.307o) (Kirkwood, 1971).…”

Section: Bloodmentioning

confidence: 99%

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Factors affecting the feeding of northern fowl mites, Ornithonyssus sylvarium, through a membrane

Crystal

1987

Entomologia Exp Applicata

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Northern fowl mites, Ornithonyssus sylviarum (Canestrini & Fanzago) (Acari: Macronyssidae), were fed artificially through chick skin membranes to investigate several factors that affected the degree of mite feeding. More mites fed after starving 24 h than unstarved mites or mites starved 48 or 72 h. Protonymphs fed as well as adults. More mites fed on refrigerated blood than on fresh blood and on frozen blood than on refrigated blood. However, mites fed well on newly drawn blood that had been lysed. They fed as well on plasma and serum as on whole blood but less well on washed erythrocytes. They also fed well on citrated blood, but fewer mites fed on oxalated blood or freshly defibrinated blood.

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“…The proteins of interest would probably be concealed due to the lack of evidence to suggest a natural immunity developed against exposed antigen, as previously mentioned. Feeding assays for D. gallinae have been developed previously (Kirkwood 1971;McDevitt et al 2006), and a modification of the McDevitt et al techniques was employed in the current study. This has enabled the preliminary testing of antibodies raised against D. gallinae proteins to determine potential vaccine candidates, before embarking on the more technically demanding and expensive process of in vivo challenge studies.…”

Section: Introductionmentioning

confidence: 99%

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

et al. 2009

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Dermanyssus gallinae (De Geer), the poultry red mite, is a blood-feeding ectoparasite that infests many bird species. We have used an in vitro feeding assay to allow the identification of protective D. gallinae antigens that may have potential as vaccine candidates. Homogenised mites were extracted sequentially with PBS, Tween 20, Triton X100 and urea giving four protein fractions. Five experimental groups of Lohmann Brown hens were used to generate antibodies; four groups were injected with one of each of the protein fractions in QuilA adjuvant and a control group was injected with adjuvant only. Booster injections were administered 2 and 4 weeks after initial immunisation. Eggs were collected throughout the experiment and soluble IgY antibodies were extracted from a pool of egg yolks collected at week six post-injection. Western blots, performed using post vaccination antibodies from test and control groups, revealed a strong antibody response against a range of injected proteins. Fresh chicken blood, supplemented with antibodies raised against these protein fractions, was fed to mites in an in vitro feeding assay in order to determine whether the antibodies had an anti-mite effect. Although there was variability in the numbers of feeding mites, it was found that the strongest anti-mite effect was seen with the PBS protein fraction, which had a cumulative average mortality of 34.8% 14 days after feeding compared with 27.3% for the control group (P = 0.043).

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In vitro feeding of Dermanyssus gallinae

Cited by 24 publications

References 18 publications

Effects of blood type and blood handling on feeding success, longevity and egg production in the body louse, Pediculus humanus humanus

Effects of blood type and blood handling on feeding success, longevity and egg production in the body louse, Pediculus humanus humanus

Factors affecting the feeding of northern fowl mites, Ornithonyssus sylvarium, through a membrane

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

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