In vitro Evaluation of Potential Calcium Phosphate Scaffolds for Tissue Engineering

Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

“…Thus, BCP exhibits a lower tendency to grow new apatite crystals. In addition, b-TCP is a soluble phase that avoids Ca 2+ depletion in media [16].…”

“…CDA powder was synthesized by the controlled crystallization method described elsewhere [16][17][18]. CDA particles were prepared by aqueous precipitation from solutions of 0.6 M (NH 4 ) 2 HPO 4 (Merck) and 0.92 M Ca(NO 3 ) 2 Á4H 2 O (Sigma, Madrid, Spain) under an air atmosphere.…”

“…Although it is known that bioactive ceramics modify Ca 2+ concentration in biological media [16], there is a lack of information addressing the influence of these particles on mesenchymal stem cell maturation through modifications in the amount of Ca 2+ available.…”

Inhibition of bacterial adhesion on biocompatible zwitterionic SBA-15 mesoporous materials

“…Samples were sterilized on each side by UV-irradiation for 1 h and then submerged in 24-well tissue culture plates (Cultek SLU, Spain) with Dulbecco's modified Eagle's medium (DMEM) (Sigma Chemical Co., St. Louis, USA) supplemented with 200 lg ml À1 penicillin (Lonza BioWhittaker, Spain) and 200 lg ml À1 streptomycin (Lonza BioWhittaker, Spain) under a 5% CO 2 atmosphere and at 37°C for 3 days in order to become stabilized before cell culture [34].…”

Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials

“…Thus, an optimum balance between the more stable phase of HA and the more soluble b-TCP exists in BCP [2], whose bioactivity is controlled by an association of HA and b-TCP in adequate ratios [3,4]. For this reason, BCP is more efficient than HA alone for different applications [5] and BCP bioceramics are recommended for use as an alternative or additive to autogenous bone for orthopedic and dental applications [1,4]. Mixtures of ceramics with agarose, as natural biodegradable binder, have been recently performed in order to increase the flexibility of the ceramic component and to facilitate the biomaterial preparation in different pieces with the desired size and shape according to the needs of the patient [6,7].…”