Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

Saldaña, Laura; Sánchez‐Salcedo, Sandra; Izquierdo‐Barba, Isabel; Bensiamar, Fátima; Munuera, L.; Vallet‐Regí, María; Vilaboa, Nuria

doi:10.1016/j.actbio.2008.11.022

Cited by 55 publications

(44 citation statements)

References 48 publications

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“…This result is consistent with the findings of a previous study showing that calcium phosphate-based particles of increasing size impaired osteogenic differentiation and bone matrix mineralization in MSC. 42 However, other research has confirmed that a high dose of zinc oxide nanoparticles is lethal to proliferating pluripotent MSC but has negligible toxicity in osteogenically differentiated MSC. 43 Nanohydroxyapatite and nanohydroxyapatite-poly(lactic-co-glycolic acid) (PLGA) composites promote osteogenic differentiation of human MSC.…”

Section: Cell Differentiationmentioning

confidence: 99%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

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Background:The purpose of this study was to investigate the influences of nanoscale wear particles derived from titanium/titanium alloy-based implants on integration of bone. Here we report the potential impact of titanium oxide (TiO 2 ) nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells (MSC) from the cellular level to the molecular level in the Wistar rat. Methods: A series of TiO 2 nanoparticles (14 nm, 108 nm, and 196 nm) were synthesized and characterized by scanning electron microscopy and transmission electron microscopy, respectively. Results: The TiO 2 nanoparticles had negative effects on cell viability, proliferation, and the cell cycle of MSC in a dose-dependent and size-dependent manner. Confocal laser scanning microscopy was used to investigate the effects of particle internalization on adhesion, spreading, and morphology of MSC. The integrity of the cell membrane, cytoskeleton, and vinculin of MSC were negatively influenced by large TiO 2 nanoparticles. Conclusion: The Transwell migration assay and a wound healing model suggested that TiO 2 nanoparticles had a strong adverse impact on cell migration as particle size increased (P , 0.01). Furthermore, alkaline phosphatase, gene expression of osteocalcin (OC) and osteopontin (OPN), and mineralization measurements indicate that the size of the TiO 2 nanoparticles negatively affected osteogenic differentiation of MSC.

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Section: Cell Differentiationmentioning

confidence: 99%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

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show abstract

“…Therefore, the chemical composition, controlled biodegradability and biologically functional properties are all important attributes for scaffolds used in TE [1][2][3] . Calcium phosphates such as hydroxyapatite (HA; Ca 10 (PO 4 ) 6 (OH) 2 ) have shown good biocompatibility and osteoconductivity 4,5 , although their mechanical strength limits their use to non-load bearing applications 6,7 . HA synthesis by wet precipitation is largely used and can be obtained by different routes.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

2011

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Hydroxyapatite-collagen (HA/Col) composites are potential scaffolds for bone tissue engineering. In this work, three-dimensional (3-D) HA/Col (50/50 wt. (%)) scaffolds were synthesized using a selfassembly method and cross-linked with a 0.125% glutaraldehyde solution. Scaffolds were evaluated in vitro by cytotoxicity testing using MC3T3 cells; proliferation and differentiation were studied using STRO-1A human stromal cells for up to 21 days. Morphological and histological examinations showed a fibrous structure with a good distribution and homogeneous HA particles distribution. By thermogravimetric analysis, a ratio of 1.2 between inorganic and organic phase was found. The scaffolds presented no cytotoxicity when evaluated using three different parameters of cell survival and integrity: 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT), Neutral Red (NR) and Crystal Violet Dye Elution (CVDE). STRO-1A cells were found to adhere, proliferate and differentiate on the 3-D scaffold, but limited cell penetration was observed.

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“…chemical conditioning) was already probed (Goshima, Goldberg et al 1991;Ohgushi, Dohi et al 1993;Yuan, Yang et al 1998;Yuan, Kurashina et al 1999;Boo, Yamada et al 2002;Kasten, Luginbuhl et al 2003;Niemeyer, Krause et al 2003;Arinzeh, Tran et al 2005;Fan, Ikoma et al 2007;Nakamura 2007;Guarino, Causa et al 2008;Cheng, Ye et al 2009;Saldana, Sanchez-Salcedo et al 2009). For example, ceramic scaffolds (i.e.…”

Section: How To Develop Informative Scaffoldsmentioning

confidence: 99%

“…For example, ceramic scaffolds (i.e. hydroxyapatite) allow a faster and more efficient cell adhesion (Goshima, Goldberg et al 1991;Ohgushi, Dohi et al 1993;Yuan, Yang et al 1998;Yuan, Kurashina et al 1999;Kasten, Luginbuhl et al 2003;Arinzeh, Tran et al 2005;Fan, Ikoma et al 2007;Cheng, Ye et al 2009;Saldana, Sanchez-Salcedo et al 2009). The synthesis of complex inorganic materials mimicking natural structures offers exciting avenues for the chemical construction of macrostructures and a new generation of biologically and structurally inspired scaffolds for tissue engineering.…”

Section: How To Develop Informative Scaffoldsmentioning

confidence: 99%

“…In this context, the bioengineering challenge become ambitious, since the complex cellbiomaterial interaction moves on multiple spatial and temporal scales. The microenvironmental cues, such as chemical environmental variables, are able to stimulate specific cellular responses at the molecular level already at early time points (Goshima, Goldberg et al 1991;Ohgushi, Dohi et al 1993;Fabbri, Celotti et al 1995;Kon, Muraglia et al 2000;Erbe, Marx et al 2001;Endres, Hutmacher et al 2003;Kasten, Luginbuhl et al 2003;Livingston, Gordon et al 2003;Niemeyer, Krause et al 2003;Arinzeh, Tran et al 2005;Kotobuki, Ioku et al 2005;Kondo, Ogose et al 2006;Fan, Ikoma et al 2007;Mygind, Stiehler et al 2007;Gigante, Manzotti et al 2008;Ng, Tan et al 2008;Bernhardt, Lode et al 2009;Saldana, Sanchez-Salcedo et al 2009). For example, ceramic scaffolds (i.e.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cell-Biomaterial Interactions Reproducing a Niche

Scaglione¹,

Giannoni²,

Quarto³

2011

Advances in Regenerative Medicine

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Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

Cited by 55 publications

References 48 publications

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

In Vitro Biological Evaluation of 3-D Hydroxyapatite/Collagen (50/50 wt. (%)) Scaffolds

Cell-Biomaterial Interactions Reproducing a Niche

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