Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials

Alcaide, María José; Serrano, María Concepción; Pagani, Raffaella; Sánchez‐Salcedo, Sandra; Vallet‐Regí, María; Portolés, María Teresa

doi:10.1016/j.biomaterials.2008.09.012

Cited by 58 publications

(51 citation statements)

References 28 publications

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“…Since SubG 1 fraction is attributed to cells with fragmented DNA, it was used as indicative of apoptosis. 14 As it can be observed in Figure 4, SubG 1 phase of cells cultured on the scaffolds increased progressively with culture time on cells cultured on nCHA scaffolds, thus indicating the induction of apoptosis by the biomaterial in osteoblasts. Nevertheless, high cell viability (80-90%) was detected in cells adhered to the scaffold surface with no changes in cell size/complexity (Fig.…”

Section: Resultsmentioning

confidence: 82%

Suppression of anoikis by collagen coating of interconnected macroporous nanometric carbonated hydroxyapatite/agarose scaffolds

Alcaide

Serrano

Román

et al. 2010

J Biomedical Materials Res

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Three dimensional interconnected macroporous (pore diameter: 600-800 μm) hydroxyapatite/agarose disks have been evaluated in this study as potential bone regeneration scaffolds. With this purpose, the adhesion and proliferation of human Saos-2 osteoblasts on this biomaterial were analyzed. As an index of cell function, the following parameters were measured: cell morphology, viability, cell size/complexity, cell cycle, reactive oxygen species (ROS) content, and lactate dehydrogenase (LDH) release. The existence of anoikis induced by inappropriate contacts between the cell and the scaffold has been detected by scanning electron microscopy, confocal microscopy, and flow cytometry. The intracellular nitric oxide content has been also measured as potential inducer of anoikis. The positive effects of previous scaffold coating with type I collagen on osteoblast adhesion as well as the collagen protection against anoikis have been demonstrated in this study.

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Section: Resultsmentioning

confidence: 82%

Suppression of anoikis by collagen coating of interconnected macroporous nanometric carbonated hydroxyapatite/agarose scaffolds

Alcaide

Serrano

Román

et al. 2010

J Biomedical Materials Res

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“…Due to the similarity in composition and crystal structure to natural bone minerals, calcium phosphates, notably hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 , HA], are widely used for bone repair [1][2][3]. After being implanted in vivo, the HA demonstrated well could chemically bond with host [4].…”

Section: Introductionmentioning

confidence: 99%

Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts

Qing

Wang

Hong

et al. 2012

J Mater Sci: Mater Med

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The effects of hydroxyapatite nanoparticles (HA-NPs) on two kinds of cells, human MG63 cells and the normal osteoblasts were investigated. According to the MTT assay and fluorescent staining assay, it was proved that HA-NPs could inhibit the growth of MG63 cells but slightly support proliferation of the osteoblasts. Meanwhile, transmission electron microscopy (TEM) was employed to observe the ultrastructural alterations of both cells. The TEM results showed that HA-NPs had entered the two kinds of cells. Typical apoptosis was observed in the MG63 cells, especially in the group of 250 μg/mL with 5 days culture. While no apoptosis could be found in the normal osteoblasts at any concentration group of HA-NPs. Our results suggested that the HA-NPs had selective effects to different kinds of cells: supporting proliferation to the normal bone cells while causing apoptosis to the osteosarcoma cells.

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“…(3) In another study, Alcaide et al reported that the presence of HA/B-Tcp in cell culture media decreased the survival rate of osteoblast-like cells but the difference was not significant and HA/B-Tcp was shown to be biocompatible with osteoblast-like cells. (13) Saldana et al evaluated the effects of Biphasic calcium phosphate (BCP) on human mesenchymal stem cells and reported that cell viability was not affected by BCP during the study (1 to 4 days) (14) Zhou et al evaluated the effects of synthetic silicate calcium phosphate on gingival fibroblasts in vitro and showed that this cement induced the formation of Hydroxyapatite crystals in Stimulated Body Fluid (SBF) with no toxicity, and that Si-cap had high biocompatibility with gingival fibroblasts. (12) Ayubian et al investigated the cell viability of Osteon, Bio-Oss, Tutudent and Cerasorb as bone graft materials using MTT assay.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of calcium silicate phosphate, osteon, bio_oss on cell viability and cell morphology of human osteoblast like cells (Saos2)

Sadatmansouri

Ayubianmarkazi

Aghajeri

et al. 2016

J Res Dentomaxillofac Sci

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ARTICLE INFOBackground and Aim: Several reports have been published on the successful application of various bone substitute materials (BSM). Appropriate physiologic and histologic characteristics and reactions of these materials against host cells are critically important. In this study, the biocompatibility of a new bone substitute material has been evaluated. Methods and Materials:In this experimental In vitro study, the biocompatibility of silicate calcium phosphate, Bio-Oss and Osteon were compared by evaluation of cell viability and differentiation rate of human osteoblast-like cell line (SaOS-2). No graft material was used in the control group. Cell viability rate was evaluated by MTT test after 1, 3 and 14 days of incubation. Inverted Light Microscope and SEM were utilized for evaluation of cell morphology. MTT and cell morphology were analyzed by ANOVA test in all groups. Results: Cell viability of the control group equaled 0.453±0.016, while in the test groups it equaled 0.453±0.016 for Bio-Oss, 0.439±0.011 for Osteon and 0.425±0.026 for silicate calcium phosphate. There was no significant difference between the control and the test groups. Spindle form was the dominant SaOS-2 cell morphology in all groups. Conclusion: This study showed that calcium silicate phosphate has appropriate biocompatibility comparable with that of Bio-Oss and Osteon.

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Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials

Cited by 58 publications

References 28 publications

Suppression of anoikis by collagen coating of interconnected macroporous nanometric carbonated hydroxyapatite/agarose scaffolds

Suppression of anoikis by collagen coating of interconnected macroporous nanometric carbonated hydroxyapatite/agarose scaffolds

Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts

Evaluation of the effect of calcium silicate phosphate, osteon, bio_oss on cell viability and cell morphology of human osteoblast like cells (Saos2)

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