In vitro evaluation of platelet concentrates, prepared from pooled buffy coats, stored for 8 days after filtration

Abstract

PCs prepared from a pool of four buffy coats can be filtered and subsequently stored for 8 days (starting +/- 24 hours after whole blood collection) without detriment to platelet function, metabolism, or activation.

Decreased platelet aggregation of platelet concentrate during storage recovers in the body after transfusion

“…There have been several reports showing changes in PLT aggregation ability, during the storage time in the PC form 7‐11 . According to these reports, the PLT aggregation ability decreased over the storage time, when aggregation was induced by thrombin, ADP, collagen, epinephrine, or PLT‐activating factor.…”

Platelets: Testing, Dosing and the Storage Lesion—Recent Advances

“…By current US Food and Drug Administration (FDA) rule, whole blood-derived platelets must be pooled prior to issue as opposed to prestorage. [7][8][9] Individually culturing multiple concentrates comprising a single platelet dose would be prohibitively cumbersome and expensive, so for whole blood-derived platelets, non-licensed, non-culture methods such as pH and glucose testing (using a dipstick or automated analyzer) and Gram staining are performed. However, the sensitivity and specificity of these non-culture methods is inferior to culture-based methods.…”

The quality of platelets after storage for 7 days

“…However, there was a reduction in response to higher concentrations of collagen by both types of PC between 4 and 7 days. Only small changes in the ability of platelets to bind to fibrinogen or collagen are observed under conditions of flow in vitro in BC PC over 8 days of storage (Boomgaard et al ., 1994c). Other studies have shown for both BC and PRP PC that adhesion to collagen is reduced to around 80–85% of day 1 values at day 7/8 (Boomgaard et al ., 1994a, 1995) and platelet adhesion to subendothelial matrix is 65% at day 3 and 45% at day 7 compared to day 1 values (Boomgaard et al ., 1994a).…”