In vitro evaluation of mitochondrial–chloroplast subcellular localization of heme oxygenase1 (HO1) in Glycine max

Dixit, Swati; Verma, Khushbu; Shekhawat, G. S.

doi:10.1007/s00709-013-0569-9

Cited by 19 publications

(6 citation statements)

References 24 publications

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“…Higher activity of antioxidants at 60 mM suggests that various antioxidants at this Values are mean ± SE (n = 3) and are statistically significant according to DMRT test (P \ 0.05). Data points marked with the different letters show significant differences (P \ 0.05) within treatments concentration work in an assembly for the plant's survival in which the major role was played by proline (Mahawar et al 2018b;Dixit et al 2014).…”

Section: Discussionmentioning

confidence: 99%

NaCl induced oxidative stress in legume crops of Indian Thar Desert: an insight in the cytoprotective role of HO1, NO and antioxidants

Khator

Mahawar

Shekhawat

2019

Physiol Mol Biol Plants

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The manuscript highlights the role of antioxidants in alleviation of salinity stress in two principal legume crops Cyamopsis tetragonoloba and Vigna radiata of Indian Thar Desert. The study evaluates correlation between the antioxidants of two cultivars in terms of morphological and physiological alterations. Hydroponically acclimatized seedlings of both the crops were subjected to NaCl stress at different concentrations ranges from 10 to 100 mM. After 96 h, the treated legumes were harvested to analyze the cellular homeostasis and salt tolerance mechanism via examining growth, stress parameters, osmoprotectants and enzymatic antioxidants. Differential response in the antioxidants activity was observed in crops. Equal contribution of antioxidants in mitigation of salinity stress was recorded in C. tetragonoloba while V. radiata shows greater tolerance by accumulating greater amount of proline which is approximately 2.72 folds higher than C. tetragonoloba. Moreover, the NR and HO1 activities in V. radiata were recorded to be 2.76 and 1.55 folds respectively which is 1.2 times higher in comparison to C. tetragonoloba. The detrimental effect of NaCl in terms of MDA content was also higher in V. radiata which concluded that V. radiata is more reactive towards salinity stress than C. tetragonoloba. The study is significant as this is the first report illustrating the sensitivity and tolerance level of NaCl in legumes of Thar Desert.

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Section: Discussionmentioning

confidence: 99%

NaCl induced oxidative stress in legume crops of Indian Thar Desert: an insight in the cytoprotective role of HO1, NO and antioxidants

Khator

Mahawar

Shekhawat

2019

Physiol Mol Biol Plants

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“…Now the genes encoding Heme oxygenase has been isolated from various living system including animals, higher plants, algae, cyanobacteria, cryptophyta and pathogenic bacteria. In higher plants HO 1 among various HO isoforms (HO 1, HO 2, HO 3 and HO 4) is the foremost stress response enzyme (Dixit et al 2014). HO 1 performs antioxidant activity under various abiotic stress including UV-B stress (Yannarelli et al 2006;Santa-Cruz et al 2017) and cadmium stress (Mahawar et al 2018a).…”

Section: Introductionmentioning

confidence: 99%

EsHO 1 mediated mitigation of NaCl induced oxidative stress and correlation between ROS, antioxidants and HO 1 in seedlings of Eruca sativa: underutilized oil yielding crop of arid region

Mahawar

Shekhawat

2019

Physiol Mol Biol Plants

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Study have focused on NaCl induced HO 1 production and its co-relation to ROS and antioxidant regulation in Eruca sativa. Seedlings were subjected to NaCl stress ranges from 10 to 150 mM. After 96 h of treatment, plants samples were harvested to evaluate the cellular equilibrium and salt tolerance mechanisms through morphological, stress parameters, non enzymatic and antioxidant enzymes. The HO 1 activity was found to be highest at 75 mM NaCl in leaves and roots which were 2.49 and 2.02 folds respectively. The expression of EsHO 1 was also observed and the higher expression was recorded in roots than leaves. The overall activity of other antioxidants (APX and proline) was also found to be higher at 75 mM concentration. The highest HO 1 activity with other antioxidants indicates the decline in LPX and ROS at 75 mM NaCl. The present study concluded that HO 1 helps in amelioration of NaCl stress by working within a group of antioxidants that create the defense machinery in seedlings of E. sativa by manipulating various physiological processes of plants. These findings for the first time suggest the protective role of HO 1 in scavenging ROS in E. sativa under salinity stress.

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“…20,21 BV generated by the HO-1 catalyzed reaction is an efficient scavenger of ROS. 22 In the soybean leaf, HO has been localized in chloroplast and mitochondria 23 and in the peribacteroid membrane of root nodules. 24 Moreover, expression of HO-1 in plants is regulated by ultraviolet-B (UV-B), H 2 O 2 , nitric oxide (NO), cytokinin, and heavy metals.…”

Section: -13mentioning

confidence: 99%

Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction

Lecube¹,

Noriega²,

Santa-Cruz³

et al. 2014

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Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in many plant processes including oxidative stress defense, but to the best of our knowledge no information is yet available about its possible action in drought stress. Methods: To this end, soybean plants were treated with 8% polyethylene glycol (PEG) or 100 μM IAA. To evaluate the behavior of IAA, plants were pretreated with this compound previous to PEG addition. Lipid peroxidation levels (thiobarbituric acid reactive substances (TBARS)), glutathione (GSH) and ascorbate (AS) contents, catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activities were determined to evaluate oxidative damage. Results: Drought treatment (8% PEG) caused a significant increase in TBARS levels as well as a marked decrease in the non-enzymatic (GSH and AS) and enzymatic (CAT, SOD, and POD) antioxidant defense systems. Pre-treatment with IAA prevented the alterations of stress parameters caused by drought, while treatment with IAA alone did not produce changes in TBARS levels, or GSH and AS contents. Moreover, the activities of the classical enzymes involved in the enzymatic defense system (SOD, CAT, and POD) remained similar to control values. Furthermore, this hormone could enhance HO-1 activity (75% with respect to controls), and this increase was positively correlated with protein content as well as gene expression. The direct participation of HO-1 as an antioxidant enzyme was established by performing experiments in the presence of Zn-protoporphyrin IX, a well-known irreversible inhibitor of this enzyme. It was also demonstrated that HO-1 is modulated by NO, as shown by experiments performed in the presence of an NO donor (sodium nitroprusside), an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide), or an NO synthesis inhibitor (N-nitro-L-arginine methyl ester, NAME). Discussion: It is concluded that IAA is responsible, at least in part, for the protection against oxidative stress caused by drought in soybean plants through the modulation of NO levels which, in turn, enhances HO-1 synthesis and activity.

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In vitro evaluation of mitochondrial–chloroplast subcellular localization of heme oxygenase1 (HO1) in Glycine max

Cited by 19 publications

References 24 publications

NaCl induced oxidative stress in legume crops of Indian Thar Desert: an insight in the cytoprotective role of HO1, NO and antioxidants

NaCl induced oxidative stress in legume crops of Indian Thar Desert: an insight in the cytoprotective role of HO1, NO and antioxidants

EsHO 1 mediated mitigation of NaCl induced oxidative stress and correlation between ROS, antioxidants and HO 1 in seedlings of Eruca sativa: underutilized oil yielding crop of arid region

Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction

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