NaCl induced oxidative stress in legume crops of Indian Thar Desert: an insight in the cytoprotective role of HO1, NO and antioxidants

Abstract

The manuscript highlights the role of antioxidants in alleviation of salinity stress in two principal legume crops Cyamopsis tetragonoloba and Vigna radiata of Indian Thar Desert. The study evaluates correlation between the antioxidants of two cultivars in terms of morphological and physiological alterations. Hydroponically acclimatized seedlings of both the crops were subjected to NaCl stress at different concentrations ranges from 10 to 100 mM. After 96 h, the treated legumes were harvested to analyze the cellular homeostasis and salt tolerance mechanism via examining growth, stress parameters, osmoprotectants and enzymatic antioxidants. Differential response in the antioxidants activity was observed in crops. Equal contribution of antioxidants in mitigation of salinity stress was recorded in C. tetragonoloba while V. radiata shows greater tolerance by accumulating greater amount of proline which is approximately 2.72 folds higher than C. tetragonoloba. Moreover, the NR and HO1 activities in V. radiata were recorded to be 2.76 and 1.55 folds respectively which is 1.2 times higher in comparison to C. tetragonoloba. The detrimental effect of NaCl in terms of MDA content was also higher in V. radiata which concluded that V. radiata is more reactive towards salinity stress than C. tetragonoloba. The study is significant as this is the first report illustrating the sensitivity and tolerance level of NaCl in legumes of Thar Desert.

Ursolic Acid Limits Salt-Induced Oxidative Damage by Interfering With Nitric Oxide Production and Oxidative Defense Machinery in Rice

“…Pretreatment of wheat seedling with glucose activates NR to produce NO under aluminum stress (Sun et al, 2018). It has been reported that endogenous NO production could influence the salinity tolerance of several plants, mostly via stimulation of antioxidant defense mechanisms (Khator et al, 2000;Ali et al, 2017;Gadelha et al, 2017;Klein et al, 2018) and suppression in uptake of toxic ions (Fatma et al, 2016;Gadelha et al, 2017). The UA-mediated endogenous NO production may be partly explained the alleviation of salt stress in rice cultivars in the present study.…”

Short term UV-B radiation mediated modulation of physiological traits and withanolides production in Withania coagulans (L.) Dunal under in-vitro condition

“…In the present study, we also demonstrated the lipid peroxidation level under UV-B exposure to analyse the oxidative damage within the cellular membrane. In W. coagulans, UV-B supplementation increased MDA content gradually with UV-B exposure, which is a well-known indicator of membrane damage in plant cells (Khator et al 2020 ). Significant increase in MDA content after 3 h UV-B exposure suggested that UV-B radiation created oxidative damage cannot be counteracted by an antioxidative defense mechanism.…”

The Putative Auto-Inhibitory Domain of Durum Wheat Catalase (TdCAT1) Positively Regulates Bacteria Cells in Response to Different Stress Conditions

“…These metalloenzymes are highly conserved and represent the major H 2 O 2 scavenging enzyme [ 5 ]. Moreover, they are regulators of different biological and physiological processes such as growth and development, cell cycle and photosynthetic functions as well as plant responses to biotic and abiotic stresses [ 5 , 6 , 7 ]. In plants, CATs are encoded by a multigenic family [ 8 ].…”