2017
DOI: 10.1590/0037-8682-0032-2017
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In vitro evaluation of hydrolytic enzyme activity and biofilm formation of Candida parapsilosis species complex from a nosocomial environment

Abstract: Introduction: Candida parapsilosis complex species, frequently found in hospital environments, have gained importance as etiological agents of candidemia. Methods: Candida parapsilosis complex isolates from a nosocomial environment were identified and their hydrolitic enzyme activity and ability to form biofilm were characterized. Results: Twenty-two C. parapsilosis sensu stricto isolates produced proteinase and three produced phospholipase. Most Candida metapsilosis isolates produced proteinase and one also p… Show more

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Cited by 7 publications
(11 citation statements)
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References 14 publications
(14 reference statements)
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“…Previously, Ramos et al showed that 15 out of 16 C. parapsilosis clinical isolates, derived from cases of cutaneous candidiasis, actively secreted aspartyl proteases (Saps) (192). Similarly, additional studies reported that Ͼ88% of C. parapsilosis clinical isolates actively secreted these enzymes (168,(193)(194)(195). On the other hand, others reported a lower number (Ͻ37%) of clinical strains producing hydrolytic proteases (196)(197)(198)(199).…”
Section: Hydrolytic Enzyme Productionmentioning
confidence: 99%
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“…Previously, Ramos et al showed that 15 out of 16 C. parapsilosis clinical isolates, derived from cases of cutaneous candidiasis, actively secreted aspartyl proteases (Saps) (192). Similarly, additional studies reported that Ͼ88% of C. parapsilosis clinical isolates actively secreted these enzymes (168,(193)(194)(195). On the other hand, others reported a lower number (Ͻ37%) of clinical strains producing hydrolytic proteases (196)(197)(198)(199).…”
Section: Hydrolytic Enzyme Productionmentioning
confidence: 99%
“…C. parapsilosis also secretes phospholipases albeit with wide intraspecies variation, with studies reporting that 9 to 90% of clinical isolates produce the enzyme (193,196,198,199). However, their contribution to pathogenicity is still a matter of debate, as there is no direct link between their expression and the degree of virulence.…”
Section: Hydrolytic Enzyme Productionmentioning
confidence: 99%
“…Nos últimos anos, casos de candidíase invasiva causada por isolados NSA têm sido relatados no Brasil (Da Costa et al, 2014;Giacobino et al, 2016;Pinhati et al, 2016;Alencar et al, 2017 As três espécies do complexo C. parapsilosis podem formar biofilme, no entanto, tal capacidade e o tipo de estrutura formada, dependem da cepa envolvida (Cordeiro et al, 2017;Cavalheiro e Teixeira, 2018). Apesar desta dependência, os isolados com maior produção de biofilme são predominantemente de C. parapsilosis (sensu stricto) (Neji, Hadrich, et al, 2017;Paula-Mattiello et al, 2017;Brilhante et al, 2018), enquanto a menor produção tem sido observada principalmente em C. metapsilosis (Cordeiro et al, 2014;Neji, Hadrich, et al, 2017;Brilhante et al, 2018) (Lattif et al, 2010;Melo et al, 2011;Treviño-Rangel et al, 2015), ao demonstrar a falta de relação da biomassa e da viabilidade celular dos biofilmes de C. parapsilosis (sensu lato), ou seja, a atividade metabólica parece ser independente da quantidade de biomassa.…”
Section: Discussionunclassified
“…Muitos micro-organismos crescem predominantemente na forma de biofilme, caracterizado por uma comunidade microbiana envolvida por uma matriz extracelular, e frequentemente aderida a superfícies sólidas (bióticas e abióticas), favorecendo a sobrevivência, inclusive em dispositivos médicos (Nobile e Johnson, 2015;Silva et al, 2017;Iñigo e Del Pozo, 2018). Estas comunidades microbianas são praticamente ubíquas, sendo observadas em condições experimentais, e naturais, como em interfaces líquido-ar (Nobile e Johnson, 2015;Del Pozo, 2018).…”
Section: Introductionunclassified
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