Complexo Candida parapsilosis: identificação molecular das espécies, análise proteômica dos biofilmes por MALDI-TOF MS e investigação de um surto envolvendo isolados clínicos resistentes aos azólicos

Thomaz, Danilo Yamamoto

doi:10.11606/t.5.2019.tde-13022019-163819

Cited by 1 publication

(1 citation statement)

References 139 publications

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“…(2016) evaluated isolates of C. parapsilosis stricto sensu (producing and nonproducing biofilms) and found no significant differences between them, showing a low reproducibility of this technique, even when different types of matrices were used for the analysis. However, as Thomaz (2018) suggested, the mentioned authors used a liquid culture medium to study the isolates, and the formation of biofilm on the wall of the bottles containing this medium could justify the absence of a difference between the samples analysed. Finally, De Carolis et al .…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of biofilm‐producing Candida species via MALDI‐TOF mass spectrometry

et al. 2021

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Aims The aim of this study was to evaluate the formation of biofilm by Candida spp. isolated from the bloodstream, using traditional spectrophotometric methodologies. In addition, the goal was to compare the results with those obtained through MALDI‐TOF/MS, as well as to verify its use as a potential tool for the detection of biofilm‐forming strains. Methods and results Hundred and thirteen isolates of Candida spp. were studied: 41 were Candida albicans, 27 C. tropicalis, 18 C. glabrata, 17 C. parapsilosis and 10 C. krusei. Metabolic activity was determined through the tetrazolium salt (XTT) reduction assay and biomass by staining with Crystal Violet. All isolates were able to form biofilm, 94% of which were strong producers, with high biomass quantification (95%; 107/113) and high metabolic activity (99%; 112/113). Mass spectra of the biofilm‐producing isolates showed differences in the intensity of mass peaks when compared with the spectra of the nonproducing strains. Conclusions It was demonstrated that MALDI‐TOF/MS was able to detect specific biofilm proteins, as the mass spectra of the isolates presented differences when compared with nonproducing strains. Significance and Impact of the Study MALDI‐TOF/MS can become a valuable tool for biofilm detection at the moment of the identification of the microorganism, thus contributing greatly to the management of patients with Candidemia.

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Section: Discussionmentioning

confidence: 99%