Rapid detection of biofilm‐producing
            <i>Candida</i>
            species via MALDI‐TOF mass spectrometry

Aguiar, P.A.D.F.; Menezes, Ralciane de Paula; Penatti, Máario Paulo Amante; Moreira, Tomaz Aquino; Pimenta, J.P.; Röder, D.V.D.B.

doi:10.1111/jam.15066

Cited by 8 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were able to identify 72% of bacteria at species level and 83% at genus levels with high confidence. Aguiar et al [ 33 ] detected specific biofilm proteins with MALDI-TOF MS and suggested the suitability of using MALDI-TOF MS for biofilm-forming yeast detection. Kačániová et al [ 34 , 35 ] demonstrated the changes in protein profile analysed by MALDI-TOF MS in their previous studies and the inhibitory effects of Coriandrum sativum and Citrus aurantium EOs on antimicrobial activity and biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver

Galovičová

Borotová

Válková

et al. 2021

Plants

View full text Add to dashboard Cite

The aim of this study was to analyze the chemical composition and biological and antibiofilm activity of the essential oil (EO) of Thymus serpyllum with the use of a MALDI-TOF MS Biotyper. The main compounds of the EO were thymol, 18.8%; carvacrol, 17.4%; o-cymene, 15.4%; and geraniol, 10.7%. It was found that free-radical scavenging activity was high. The highest antimicrobial activity was observed against Pseudomonas aeruginosa, Salmonella enteritidis, and biofilm-forming bacteria. The changes in the biofilm structure after T. serpyllum EO application confirmed the inhibitory action and the most pronounced effect was observed on Bacillus subtilis biofilm. The antifungal activity of the vapor phase was the most effective against Penicillium crustosum. T. serpyllum should be a suitable alternative to synthetic antioxidants as well as antimicrobials. The EO of T. serpyllum can be used in the vapor phase in the storage of root vegetables as well as a growth inhibitor of Penicillium on bread.

show abstract

Section: Discussionmentioning

confidence: 99%

Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver

Galovičová

Borotová

Válková

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…albicans and in a significant number of strains. The second study was performed on 113 different Candida spp., 107 (95%) of which were high-biofilm-producing (CV absorbance >1.17) and in which the authors demonstrated that MALDI-TOF MS was able to differentiate between specific biofilm proteins, subsequently validating the correlation between 4 high-producing strains and 4 low-producing strains ( Aguiar, 2021 ). We also validated this result using a larger number of strains (51), revealing a correlation of 67.7%.…”

Section: Discussionmentioning

confidence: 90%

“…Moreover, MALDI-TOF MS is already being used to identify and differentiate between other intra-species characteristics, such as antibiotic resistance profile, association with virulence genes, or even subspecies ( Weis et al, 2020 , 2022 ). To date, few studies have examined the ability of MALDI-TOF MS to differentiate between strains according to whether they have high or low biofilm-forming capacity, and available data are limited in terms of the species tested ( Mlynáriková et al, 2016 ; Caputo et al, 2018 ; De Carolis et al, 2019 ; Aguiar, 2021 ). Moreover, no studies have assessed the use of MALDI-TOF MS for determination of biofilm production in bacteria and fungi from patients with bloodstream infections.…”

Section: Introductionmentioning

confidence: 99%

Determination of the ability of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify high-biofilm-producing strains

Rodríguez-Temporal

Díez²,

Díaz-Navarro³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

BackgroundThe traditional method for assessing the capacity of a microorganism to produce biofilm is generally a static in vitro model in a multi-well plate using the crystal violet (CV) binding assay, which takes 96 h. Furthermore, while the method is simple to perform, its reproducibility is poor.ObjectiveWe evaluated whether matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) could make it possible to differentiate between high-and low-biofilm-producing microorganisms on 24-h cultures of Staphylococcus aureus and Candida albicans.MethodsWe included 157 strains of S. aureus and 91 strains of C. albicans obtained from the blood cultures of patients with bacteremia/candidemia. We tested biofilm production using the CV binding assay as the gold standard to classify strains as low or high biofilm producers. We then applied MALDI-TOF MS to create a machine learning–based predictive model using 40 strains of S. aureus and C. albicans, each with extreme absorbance values, and validated this approach with the remaining 117 and 51 strains using the random forest algorithm and the support vector machine algorithm, respectively.ResultsOverall, 81.2% of the S. aureus strains (95/117) and 74.5% of the C. albicans strains (38/51) used for validation were correctly categorized, respectively, as low and high-biofilm-producing.ConclusionClassification based on MALDI-TOF MS protein spectra enables us to predict acceptable information about the capacity of 24-h cultures of S. aureus and C. albicans to form biofilm.

show abstract

“…Moreover, scanning electron microscopy or confocal microscopy are used in the evaluation of the density of the biofilm in centers where the infrastructure is sufficient. In the evaluation of biofilm formation in Candida species, the use of proteomic analyzes with the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has come to the fore, and more successful results have been obtained in the demonstration of biofilm compared to standard methods (19).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Crystal Violet Staining Assay and XTT Methods in the Evaluation of Biofilm Formation in Candida parapsilosis Candidemia Isolates

Doğan

Ataç

Babuççu

et al. 2021

Infect Dis Clin Microbiol

View full text Add to dashboard Cite

Objective: Biofilm formation is one of the most important virulence factors of Candida species which leads to permanent infection foci by adhering to foreign materials and which are difficult to treat. Candida parapsilosis, which is one of the most common causes of candidemia in our country, is frequently isolated as a causative agent in catheterrelated infections. The most commonly used methods for evaluating the biofilm formation of Candida species are measuring cell viability with XTT (2,3-bis (2-methoxy-4-nitro-5sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) and evaluating the total biofilm mass with crystal violet (CV). The aim of this study is to evaluate the biofilm formation ability of C. parapsilosis candidemia isolates by XTT and (CV) methods and compare these methods with each other.Materials and Methods: C. parapsilosis isolates sent from various hospitals between 2015 and 2019 were included in the study retrospectively, and the species-level identification was performed using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) The biofilm formation of the isolates was compared based on the optical density (OD) values obtained by crystal violet and XTT methods. The biofilm formation of the isolates was evaluated by categorizing them into low, medium and high biofilm groups as ± 20% according to the median value of all strains.Results: Totally, 79 C. parapsilosis candidemia isolates were included in this study and categorical compatibility between CV and XTT methods in low, medium and high biofilm groups was found as 69.6%, 60.6% and 73.9%, respectively. The OD values of the XTT method in the high biofilm group were found statistically significantly higher when compared with the values from the CV method. Conclusion:The compatibility of XTT and crystal violet methods in terms of biofilm measurement in C. parapsilosis isolates was considered acceptable, and no major variations were detected between the categories. According to these results, when evaluating the biofilm levels of C. parapsilosis isolates, high OD values obtained by the XTT method should be confirmed with the CV method.

show abstract

Rapid detection of biofilm‐producing Candida species via MALDI‐TOF mass spectrometry

Cited by 8 publications

References 34 publications

Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver

Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver

Determination of the ability of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify high-biofilm-producing strains

Comparison of Crystal Violet Staining Assay and XTT Methods in the Evaluation of Biofilm Formation in Candida parapsilosis Candidemia Isolates

Contact Info

Product

Resources

About