In vitro evaluation of biodegradation of poly(lactic-co-glycolic acid) sponges

Yoshioka, Taiyo; Naoki, Katsuhiko; Tateishi, Tetsuya; Chen, Guoping

doi:10.1016/j.biomaterials.2008.04.011

Cited by 83 publications

(72 citation statements)

References 33 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ideal scaffolds must allow for proper cell adhesion and growth in combination with the necessary porosity, biocompatibility, biodegradability, and mechanical strength that are required for use in biological systems 3) . Poly (lactic-co-glycolic acid) (PLGA) was approved for use by the Food and Drug Administration (FDA) as a popular scaffold, o wi n g t o i t s a d j u s t a b l e d e gr a d a t i o n r a t e a n d g o o d biocompatibility 4) . However, its poor hydrophilicity and lacking of cell anchorage sites has been shown to impede cell attachment and penetration of the scaffold 5) .…”

Section: Introductionmentioning

confidence: 99%

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Zheng

Wang

Qin

et al. 2015

J. Hard Tissue Biology.

View full text Add to dashboard Cite

The temperature-sensitive triblock copolymer poly-(D, L-lactic acid-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-PLGA (PLGA-PEG-PLGA) is an FDA-approved material that has the ability to provide a sustained release of drugs and/or proteins. Platelet-rich fibrin(PRF)is second generation platelet concentration that contains growth factors such as transforming growth factor-1 (TGF-1), platelet derived growth factor-AB (PDGF-AB), and insulin-like growth factor-I (IGF-I). These growth factors affect the migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study sought to combine the hydrogel into scaffolds in order to serve as a sustained release system for PRF-derived growth factors. Poly (lactic-co-glycolic) acid (PLGA) and nano-hydroxyapatite (nHA) were used to prepare the hydrogel-containing scaffolds with the PRF-derived growth factors. We then investigated the effects of the hydrogel on modulating the activity of osteoblasts in vitro. We indicated that the hydrogel (Gel) was well-distributed in the inner surface of scaffolds, which themselves exhibited relatively interconnected pores with uniform sizes. The addition of the hydrogel didn't affect their inherently high porosity. In vitro release tests indicated that the system containing nHA/PLGA/Gel/PRF provided for a slow and sustained release of PRF-derived growth factors. The results from our in vitro studies indicated that the MG63 cells cultured with both scaffold media extracts did not appear to have cytotoxic responses, and the nHA/PLGA/Gel/PRF system could improve the adhesion and proliferation of MG63 cells when compared to controls (p < 0.05). This in vitro evaluation suggests that the hydrogel-scaffold system is suitable as a model for bone tissue engineering, and that it allows for the sustained release of growth factors to improve bone reconstruction.

show abstract

Section: Introductionmentioning

confidence: 99%

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Zheng

Wang

Qin

et al. 2015

J. Hard Tissue Biology.

View full text Add to dashboard Cite

show abstract

“…19 Many factors can affect PLGA degradation, such as (i) molecular weight, (ii) co-polymer composition, (iii) stereochemistry, (iv) end-group functionalization, (v) chemical derivatization, (vi) geometry of the material, and (vii) characteristics of the surrounding medium. 20 PLGA chain length is a very important parameter to be considered when selecting the adequate polymer properties for specific applications, as the physical strength and degradation speed of PLGA depend to a large extent on its molecular weight. By increasing the molecular weight of conventional PLGAs from 10-20 to 100 kDa, degradation rates were reported to range from several weeks to several months.…”

Section: Degradationmentioning

confidence: 99%

“…By increasing the molecular weight of conventional PLGAs from 10-20 to 100 kDa, degradation rates were reported to range from several weeks to several months. 20,21 The co-polymer composition (more specifically the ratio between lactic and glycolic acid) is also an important parameter that is used for controlling PLGA degradation. Lactic acid is more hydrophobic than glycolic acid, which makes that lactide-rich PLGA co-polymers are less hydrophilic, absorb less water, and, subsequently, degrade slower.…”

Section: Degradationmentioning

confidence: 99%

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Lanao

Jonker

Wolke

et al. 2013

Tissue Engineering Part B: Reviews

166

View full text Add to dashboard Cite

“…Therefore, curves point out a variation on the degradation process that could obey a preferential degradation of the amorphous region at the beginning that is followed by a slower degradation of the edge of crystallites [33][34][35]. In fact, the first step can be associated to a quick random chain scission mechanism that follows a first-order kinetic, irrespective of the chain length, whereas a slower chain-end-scission mechanism can be expected when degradation affects the crystalline domains [29,[36][37][38][39]. Note in Figure 5b the differences between the experimental curve and the simulated one considering a theoretical first order equation.…”

Section: Hydrolytic Degradation Of Gl-b-[gl-co-tmc-co-cl]-b-gl In Difmentioning

confidence: 99%

Influence of pH on Morphology and Structure during Hydrolytic Degradation of the Segmented GL-b-[GL-co-TMC-co-CL]-b-GL Copolymer

Márquez

Martínez

Turón³

et al. 2015

Fibers

View full text Add to dashboard Cite

Hydrolytic degradation in media having a continuous variation of pH from 2 to 12 was studied for a copolymer having two polyglycolide hard blocks and a middle soft segment constituted by glycolide, trimethylene carbonate, and ɛ-caprolactone units. The last units were susceptible to cross-linking reactions by γ irradiation that led to an increase of the molecular weight of the sample. Nevertheless, the susceptibility to hydrolytic degradation was enhanced with respect to non-irradiated samples and consequently such samples were selected to analyze the degradation process through weight loss measurements and the evaluation of changes on molecular weight, morphology, and SAXS patterns. Results reflected the different hydrolytic mechanisms that took place in acid and basic media and the different solubilization of the degradation products. Thus, degradation was faster and solubilization higher in the basic media. In this case, fibers showed a high surface erosion and the formation of both longitudinal and deep circumferential cracks that contrasted with the peeling process detected at intermediate pHs (from 6 to 8) and the absence of longitudinal cracks at low pHs. SAXS measurements indicated that degradation was initiated through the OPEN ACCESSFibers 2015, 3 349 hydrolysis of the irregular molecular folds placed on the amorphous interlamellar domains but also affected lamellar crystals at the last stages. Subsequent heating processes performed with degraded samples were fundamental to reveal the changes in microstructure that occurred during degradation and even the initial lamellar arrangement. In particular, the presence of interfibrillar domains and the disposition of lamellar domains at different levels along the fiber axis for a determined cross-section were evidenced.

show abstract

In vitro evaluation of biodegradation of poly(lactic-co-glycolic acid) sponges

Cited by 83 publications

References 33 publications

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Influence of pH on Morphology and Structure during Hydrolytic Degradation of the Segmented GL-b-[GL-co-TMC-co-CL]-b-GL Copolymer

Contact Info

Product

Resources

About