New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Zheng, Lihua; Wang, Lin; Qin, Jie; Sun, Xin; Yang, Tingting; Ni, Yushan; Zhou, Yanmin

doi:10.2485/jhtb.24.54

Cited by 12 publications

(13 citation statements)

References 19 publications

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“…In contrast, it has been reported that lyophilized platelet products can release the vast majority of cytokines rapidly after 24‐h incubation (Zheng et al . ). In this study, cytokines were extracted using the lyophilized method and five representative cytokines, including TGF‐β1, PDGF‐BB, VEGF, IGF‐1 and bFGF, were detected.…”

Section: Discussionmentioning

confidence: 97%

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The potential application of concentrated growth factor in regenerative endodontics

Hong

Cai

et al. 2018

Int Endodontic J

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Aim To investigate the potential application of concentrated growth factor (CGF) to promote pulp regeneration within immature teeth. Methodology Concentrated growth factor clots produced from peripheral blood samples were investigated histologically by haematoxylin–eosin (HE) staining and evaluated morphologically by scanning electron microscope (SEM). The cytokines were extracted from the CGF, and representative cytokines were quantified by enzyme‐linked immunosorbent assay (ELISA). The biological effects of the CGF on human stem cells from the apical papilla (SCAPs) were then investigated and quantified, including cell proliferation, cell migration, mineralized nodule formation, and the gene expression of alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP) and dentine matrix protein (DMP)‐1. The results were analysed statistically using one‐way analysis of variance (anova). Results Concentrated growth factor had a complex three‐dimensional structure with a high density of platelets and nucleated cells. Representative growth factors including PDGF‐BB, IGF‐1, TGF‐β1, bFGF and VEGF were detected. The growth rate and migratory cell numbers of the CGF groups were significantly greater than those in the control groups (P < 0.05). The mineralization areas in the CGF groups were significantly larger than those in the control groups (P < 0.05). The expression levels of ALP, DSPP and DMP‐1 were significantly up‐regulated after induction by CGF (P < 0.05). Conclusions Concentrated growth factor promoted the proliferation, migration and differentiation of SCAPs and could be a promising biomaterial applied in regenerative endodontics.

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Section: Discussionmentioning

confidence: 97%

“…Growth factors extracted from the CGF were prepared according to previously reported protocols with slight modifications (Zheng et al 2015). In brief, the fresh CGF clot was placed in a lyophilizer overnight.…”

Section: Quantification Of Representative Growth Factors In the Cgfmentioning

confidence: 99%

The potential application of concentrated growth factor in regenerative endodontics

Hong

Cai

et al. 2018

Int Endodontic J

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“…The CGF gel was collected from the middle layer of the samples (between the upper, platelet-poor plasma layer, and the lower, red blood cell layer) and pressed onto a membranous film as previously described [13]. CGF conditioned media were prepared as follows [24]: CGF membranes (each membrane was prepared from 2 mL blood) were placed in lyophilizer overnight. The lyophilized CGF membranes were ground into powder and immersed into 2 mL DMEM at 37 °C for 24 h. The media were collected and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro

Zhang

2019

J Orthop Surg Res

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Objective The aim of this research is to investigate the effects of concentrated growth factor (CGF) on the proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells (PDCs) in vitro. Methods PDCs were isolated from the femoral and tibial periosteum of rabbits and cultured with or without CGF membranes or CGF conditioned media. Scanning electron microscopy (SEM) was used for the structural characterization. Cell Counting Kit-8 assay was used to measure cell proliferation. Alkaline phosphatase (ALP) activity of PDCs was also measured. Immunohistochemistry was used to detect the expression of CD34. Enzyme-linked immunosorbent assay (ELISA), quantitative real-time PCR (qPCR), and Western blot were used to evaluate the secretion and expression levels of osteogenic differentiation markers (bone morphogenetic protein-2, type I collagen, osteocalcin) and angiogenesis markers (vascular endothelial growth factor, basic fibroblast growth factor) in supernatants and PDCs at days 3, 7, 14, and 21. Results The SEM analysis showed a dense three-dimensional fibrin network in CGF, and CGF membranes were covered by PDCs with elongated or polygonal morphological features. Compared with the control group, CGF significantly promoted the proliferation of PDCs during the experimental period ( p < 0.05). Immunohistochemistry revealed that PDCs were dispersedly distributed among the CGF substrates, and CD34-positive cells were also present. Moreover, CGF significantly increased the ALP activity and upregulated the expression and secretion of osteogenic differentiation and angiogenesis markers in PDCs at days 3, 7, 14, and 21 ( p < 0.05). Conclusion CGF can increase the proliferation and promote the osteogenic differentiation and angiogenic potential of PDCs in vitro. These results indicate that CGF can be used as a new therapeutic means for biotechnological and clinical applications.

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“…LPCs express their growth factors more slowly than PCs. This was discovered in a study by Zheng [13] when they attempted to combine nano-hydroxyapatite (nHA), L-lactic acid-co-glycolic acid (PLGA), and the hydrogel (nHA/PLGA/Gel) scaffold with LPCs. The in vitro release experiments showed that the composite scaffold allowed for gradual and continuous release of PRF-derived growth factors.…”

Section: Overview Of Lyophilization For Platelet Concentratesmentioning

confidence: 99%

“…However, PCs were designed for immediate and short-term use (for up to 14-days); thus, this approach somewhat limits their therapeutic value [11,12]. As longer periods are reportedly required for the enhancement of bone regeneration, more effective methods for the delivery of growth factors over longer time-periods have been sought [13]. Furthermore, PCs are processed from autologous blood prior to clinical use, and, therefore, their bioactivity can vary between individuals.…”

Section: Introductionmentioning

confidence: 99%

Potential of Lyophilized Platelet Concentrates for Craniofacial Tissue Regenerative Therapies

et al. 2021

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Objective: The use of platelet concentrates (PCs) in oral and maxillofacial surgery, periodontology, and craniofacial surgery has been reported. While PCs provide a rich reservoir of autologous bioactive growth factors for tissue regeneration, their drawbacks include lack of utility for long-term application, low elastic modulus and strength, and limited storage capability. These issues restrict their broader application. This review focuses on the lyophilization of PCs (LPCs) and how this processing approach affects their biological and mechanical properties for application as a bioactive scaffold for craniofacial tissue regeneration. Materials and Methods: A comprehensive search of five electronic databases, including Medline, PubMed, EMBASE, Web of Science, and Scopus, was conducted from 1946 until 2019 using a combination of search terms relating to this topic. Results: Ten manuscripts were identified as being relevant. The use of LPCs was mostly studied in in vitro and in vivo craniofacial bone regeneration models. Notably, one clinical study reported the utility of LPCs for guided bone regeneration prior to dental implant placement. Conclusions: Lyophilization can enhance the inherent characteristics of PCs and extends shelf-life, enable their use in emergency surgery, and improve storage and transportation capabilities. In light of this, further preclinical studies and clinical trials are required, as LPCs offer a potential approach for clinical application in craniofacial tissue regeneration.

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New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Cited by 12 publications

References 19 publications

The potential application of concentrated growth factor in regenerative endodontics

The potential application of concentrated growth factor in regenerative endodontics

Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro

Potential of Lyophilized Platelet Concentrates for Craniofacial Tissue Regenerative Therapies

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