Abstract:The aim of this work was to study the probiotic-related characteristics of four strains of bacteria isolated from the normal flora of the guppy, Poecilia reticulata. In vitro results showed that the strains, namely, MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 had higher adhesion abilities than the tested indicator strains. However, an association between the cell-surface hydrophobicity and the ability to adhere to the intestinal mucus was not observed for these strains. Further, the selected strains were strongl… Show more
“…The obtained results were in agreement with those reported by Blakrishma (36); as he showed that there is no correlation between these parameters. In another study, Lp9 isolate has surface hydrophobicity of 37-38%, suggesting its adhesiveness (37).…”
Background
For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections.
Objectives
Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes.
Materials and Methods
The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides. The best strain was identified by PCR.
Results
From 70 lactobacilli isolates and according to the 16 rDNA sequences, isolates B6 and B10 showed the closest homology (99%) to the Lb. gasseri and Lb. plantarum respectively. They produced hydrogen peroxide (H2O2), tolerant to acid, bile, simulated human intestinal fluid, present a strong adhesion, highest percentages of aggregation, and antibacterial activity. These strains are resistant to the spermicide and actively acidify the growth medium.
Conclusions
Strains Lb. plantarum B10 and Lb. gasseri B6 have a strong potential probiotic confirming their value as a tool for prevention against urinary and vaginal infections.
“…The obtained results were in agreement with those reported by Blakrishma (36); as he showed that there is no correlation between these parameters. In another study, Lp9 isolate has surface hydrophobicity of 37-38%, suggesting its adhesiveness (37).…”
Background
For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections.
Objectives
Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes.
Materials and Methods
The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides. The best strain was identified by PCR.
Results
From 70 lactobacilli isolates and according to the 16 rDNA sequences, isolates B6 and B10 showed the closest homology (99%) to the Lb. gasseri and Lb. plantarum respectively. They produced hydrogen peroxide (H2O2), tolerant to acid, bile, simulated human intestinal fluid, present a strong adhesion, highest percentages of aggregation, and antibacterial activity. These strains are resistant to the spermicide and actively acidify the growth medium.
Conclusions
Strains Lb. plantarum B10 and Lb. gasseri B6 have a strong potential probiotic confirming their value as a tool for prevention against urinary and vaginal infections.
“…Furthermore, the coaggregation and AAg were found to be directly related properties. The results are in agreement with earlier reports [6,36]. Nature of cell surface components of probiotics/pathogens may account for differences in coaggregation and AAg [1,2].…”
Section: Discussionsupporting
confidence: 93%
“…Coaggregation ability of probiotics with pathogens is considered as a desired property as it may play an important role in eliminating the pathogens from the GIT by preventing their attachment/lodging to the host tissue [35]. Varying level of coaggregation ability of the LAB isolates with different pathogens indicated that coaggregation potential is specific attribute that depends upon type of probiotic strain and the specific pathogens [36]. Furthermore, the coaggregation and AAg were found to be directly related properties.…”
Wide variations among multifaceted-health benefitting attributes of probiotics fueled investigations on targeting efficacious probiotics. In the current study, lactic acid bacteria (LAB) isolated from poultry gut, feces of rat, chicken, human infants, and fermented foods were characterized for desired probiotic functional properties including the phytase-producing ability which is one of the wanted characteristics for probiotics for potential applications for upgrading animal nutrition, enhancing feed conversion, and minimizing anti-nutritional properties. Among 62 LAB isolates Weissella kimchii R-3 an isolate from poultry gut exhibited substantial phytase-producing ability (1.77 U/ml) in addition to other functional probiotic characteristics viz. hydrophobicity, autoaggregation, coaggregation with bacterial pathogens, and antimicrobial activity against pathogens. Survival of W. kimchii R-3 cells (in free and calcium alginate encapsulated state) was examined sequentially in simulated gastric and intestinal juices. Encapsulated cells exhibited better survival under simulated gut conditions indicating that encapsulation conferred considerable protection against adverse gut conditions. Furthermore, simulated gastric and intestinal juices with pepsin and pancreatin showed higher survival of cells than the juices without pepsin and pancreatin. W. kimchii R-3 due to its significant functional probiotic attributes may have prospective for commercial applications in human/animal nutrition.
“…The results revealed that aggregation, hydrophobicity and adhesion of LAB strains are strain‐specific and the correlations between autoaggregation and hydrophobicity, and autoaggregation and adhesion are positive among the nine L. lactis strains rather than the 18 strains belonging to different species tested. Therefore, bacterial aggregation and hydrophobicity can be used as a preliminary screening to assess their adhesion properties (Balakrishna ). In addition, porcine ileal epithelial cells were used in in vitro models to investigate aggregation and adhesiveness of Lactobacillus acidophilus M92 (Kos et al .…”
Eggs, gills, skin and intestinal tracts of fish harbour bacterial communities that are thought to impact fish health through their interaction with the tissues. In particular, the gastrointestinal (GI) tract possesses numerous microbes and is considered an infection route. During the infections caused by pathogenic bacteria, mucosal adhesion is a critical early phase and plays an important role in fish development. This review focused on knowledge regarding the adherence and colonization of microorganisms in fish GI tract mucus, including various methods such as in vivo, in vitro, and ex vivo, host specificity, lactic acid bacteria nonfish origin using the adherence of pathogenic bacteria, gnotobiotic approaches and molecular methods. However, probiotics might not be possible to truly colonize the digestive tract of fish. The term 'temporal colonization' might be a better phrase to use when discussing probiotic persistence and presence within the mucosal layer. Recent and promising results in this field were presented based on new perspectives and future research directions of the adherence and colonization of microorganisms in fish GI tracts.
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