In vitro effects of genistein and resveratrol on the production of interferon-γ (IFNγ) and interleukin-10 (IL-10) by stimulated murine splenocytes

Rachoń, Dominik; Rimoldi, Guillermo; Wuttke, W.

doi:10.1016/j.phymed.2005.10.006

Cited by 25 publications

(15 citation statements)

References 39 publications

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“…Resveratrol also inhibited the production of IFNγ and IL-10 from mouse splenocutes stimulated with 12-myristate 13-acetate (PMA) and ionomycin. 103 Resveratrol also modulates the function of G protein-coupled chemo attractant receptors, which play important roles in inflammation and immune responses. In micromolar concentrations, it potently reduced the production of superoxide anion by phagocytic leukocytes in response to the bacterial chemotactic peptide fMLF, a high affinity ligand for formylpeptide receptor FPR, and A beta42, an Alzheimer's disease-associated peptide.…”

Section: Resveratrol and Immune Systemmentioning

confidence: 99%

Resveratrol: A multitargeted agent for age-associated chronic diseases

Harikumar¹,

Aggarwal²

2008

Cell Cycle

481

339

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Extensive research within the last decade has revealed that most chronic illnesses such as cancer, cardiovascular and pulmonary diseases, neurological diseases, diabetes, and autoimmune diseases exhibit dysregulation of multiple cell signaling pathways that have been linked to inflammation. Thus mono-targeted therapies developed for the last two decades for these diseases have proven to be unsafe, ineffective and expensive. Although fruits and vegetables are regarded to have therapeutic potential against chronic illnesses, neither their active component nor the mechanism of action is well understood. Resveratrol (trans-3, 5, 4'-trihydroxystilbene), a component of grapes, berries, peanuts and other traditional medicines, is one such polyphenol that has been shown to mediate its effects through modulation of many different pathways. This stilbene has been shown to bind to numerous cell-signaling molecules such as multi drug resistance protein, topoisomerase II, aromatase, DNA polymerase, estrogen receptors, tubulin and F1-ATPase. Resveratrol has also been shown to activate various transcription factor (e.g., NFκB, STAT3, HIF-1α, β-catenin and PPAR-γ), suppress the expression of antiapoptotic gene products (e.g., Bcl-2, Bcl-X L , XIAP and survivin), inhibit protein kinases (e.g., src, PI3K, JNK and AKT), induce antioxidant enzymes (e.g., catalase, superoxide dismutase and hemoxygenase-1), suppress the expression of inflammatory biomarkers (e.g., TNF, COX-2, iNOS and CRP), inhibit the expression of angiogenic and metastatic gene products (e.g., MMPs, VEGF, cathepsin D and ICAM-1), and modulate cell cycle regulatory genes (e.g., p53, Rb, PTEN, cyclins and CDKs). Numerous animal studies have demonstrated that this polyphenol holds promise against numerous age-associated diseases including cancer, diabetes, Alzheimer, cardiovascular and pulmonary diseases. In view of these studies, resveratrol's prospects for use in the clinics are rapidly accelerating. Efforts are also underway to improve its activity in vivo through structural modification and reformulation. Our review describes various targets of resveratrol and their therapeutic potential.

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Section: Resveratrol and Immune Systemmentioning

confidence: 99%

Resveratrol: A multitargeted agent for age-associated chronic diseases

Harikumar¹,

Aggarwal²

2008

Cell Cycle

481

339

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“…Possible candidates for the beneficial actions of soybean compounds are isoflavones, such as genistein and daidzein, since they show a structural similarity to oestradiol (Rachoń et al 2006). Recent work from our group has found that these compounds stimulate key immune functions in ovariectomized mice (Baeza et al 2007(Baeza et al , 2009.…”

Section: Phytoestrogensmentioning

confidence: 99%

Effects of growth hormone, melatonin, oestrogens and phytoestrogens on the oxidized glutathione (GSSG)/reduced glutathione (GSH) ratio and lipid peroxidation in aged ovariectomized rats

et al. 2010

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Ovariectomy constitutes a commonly used model in rats and mice for human menopause. After ovariectomy, an imbalance between oxidant production and antioxidant levels appears in favour of the former, with increased oxidative stress and consequently an acceleration of ageing. In the present work, the levels of reduced glutathione (GSH), a relevant antioxidant, and oxidized glutathione (GSSG), an oxidant compound, as well as lipid peroxidation (through malondialdehyde (MDA) levels), were studied in liver, heart, kidney and spleen homogenates of old (24 months of age) unovariectomized and ovariectomized female Wistar rats. The results showed a significant increase of the GSSG/GSH ratio, a marker of oxidative stress, and higher MDA production in all the studied organs of ovariectomized rats as compared with unovariectomized animals. These data confirm the idea that ovariectomy accelerates the ageing process. Administration of growth hormone (GH), melatonin (MEL) and oestrogens (OE), as well as soybean phytoestrogens (PE) for 10 weeks, between 22 and 24 months of age, was able to decrease oxidative stress in the investigated organs of ovariectomized old rats, therefore slowing down the ageing process in those animals.

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“…The observed shift of the Th1/Th2 balance towards a Th2 type response demonstrated the anti-inflammatory activity of V. cinerea 21 . The mechanisms of cytokine production might be related to gene expression, mRNA stability, or the synthesis, stability, and production of proteins, however, the exact mechanism of action required clarification.…”

Section: Discussionmentioning

confidence: 77%

Anti-inflammatory activity of a Vernonia cinerea methanolic extract in vitro

Saraphanchotiwitthaya¹,

Sripalakit²

2015

ScienceAsia

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Vernonia cinerea Less. (Asteraceae) is a herbaceous plant commonly found in SE Asia. Different parts of V. cinerea have been used in various folklore medicine applications, including reducing inflammation and detoxification. This study investigates the effects of V. cinerea methanolic extract on the mouse immune system in vitro. Cytokine detection of ConA-stimulated splenocytes using ELISA, mitogen-induced splenocyte proliferation, and phagocytic activity were assayed. The results show that the extract shifted the Th1/Th2 balance towards a Th2 type response. Additionally, Th1-and Th2-related cytokine production correlated with lymphocyte proliferation by selective activation of T-cells into Th2-cells and suppression of Th1-cell proliferation. Our results confirmed the traditional applications of V. cinerea on immune-related remedies and anti-inflammation, suggesting a potential therapeutic application of this plant in the treatment of diseases associated with Th1-related cytokine production and inflammation.

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In vitro effects of genistein and resveratrol on the production of interferon-γ (IFNγ) and interleukin-10 (IL-10) by stimulated murine splenocytes

Cited by 25 publications

References 39 publications

Resveratrol: A multitargeted agent for age-associated chronic diseases

Resveratrol: A multitargeted agent for age-associated chronic diseases

Effects of growth hormone, melatonin, oestrogens and phytoestrogens on the oxidized glutathione (GSSG)/reduced glutathione (GSH) ratio and lipid peroxidation in aged ovariectomized rats

Anti-inflammatory activity of a Vernonia cinerea methanolic extract in vitro

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