In vitro effect of lemon and orange juices on calcium oxalate crystallization

Abstract

To investigate the inhibitory effect of lemon and orange juices on the growth of struvite crystals. Struvite crystals were grown by the single diffusion gel growth technique and the inhibitory effect of lemon and orange juices on the growth of struvitecrystals has been studied. With an increase in the concentration of lemon and orange juices, the weight of the formed crystals was gradually reduced from 2.49 g to 0.09 g (lemon juice) and 2.49 g to 0.21 g (orange juice) for the struvite crystals, respectively. The crystals harvested from the treated lemon and orange juices were characterized by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups. Results obtained indicated that lemon juice has the potential to inhibit the formation of struvitecrystals as compared to orange juice. This study confirms that using lemon juice can promote the formation of ammonium magnesium phosphate hexahydratecrystals and reduce the nucleation rate of struvitecrystals, a major component of urinary stone.

Effect of theCopaifera langsdorffiiDesf. Leaf Extract on the Ethylene Glycol-Induced Nephrolithiasis in Rats

“…In this experiment, approximately 30 min after mixing the calcium chloride and sodium oxalate solutions there was maximum increase in the optical density, which reflects the maximum crystal nucleation rate. After the solution reached equilibrium, the optical density became progressively lower, due to the diminished turbidity that resulted from the utilization of disperse particles in crystal aggregation [21]. In other words, decreased optical density was the outcome of enhanced crystal aggregation.…”

“…The in vitro effect of the copaiba leaf extract on the nucleation and aggregation of calcium oxalate monohydrate crystals was studied by using a previously described turbidimetric method [21]. Briefly, calcium oxalate monohydrate crystallization was obtained by mixing 12.5 mL calcium chloride (8 mmol/L) into 12.5 mL sodium oxalate (1 mmol/L) and sodium acetate/acetic acid buffer 10 mmol/L (pH 5.7).…”

Antioxidant Properties of Polysaccharide from the Brown Seaweed Sargassum graminifolium (Turn.), and Its Effects on Calcium Oxalate Crystallization

“…This assay quantifies crystal nucleation and aggregation in metastable solutions of Ca 2+ and Ox. There are three parameters that characterize the crystallization process [36]: first, the maximum increase of optical density (OD) over time, termed S N , mainly reflects the maximum rate of formation of new particles and thus, represents crystal nucleation. Second, the rate of aggregation, S A , is derived from the maximum decrease in optical density.…”

Herbal Medicines in the Management of Urolithiasis: Alternative or Complementary?

“…Dietary factors appear to affect the ability of urine to inhibit CaOx crystallization. In this regard, lemon juice has been found to inhibit the rate of crystal nucleation and aggregation [38,39]. Overall, the in vitro crystallization experiments confirmed that prophylaxis of renal stones could be achieved by reducing supersaturation through promotion of small crystal nucleates.…”