Effect of theCopaifera langsdorffiiDesf. Leaf Extract on the Ethylene Glycol-Induced Nephrolithiasis in Rats

Abstract

The potential of the Copaifera langsdorffii leaves extract to prevent stone formation was analyzed by means of an ethylene glycol (EG) animal model of nephrolithiasis and an in vitro crystallization assay. Different doses of the C. langsdorffii leaves extract were administered to rats treated with EG. Urine biochemical parameters were quantified. CaOx deposits count and analysis of osteopontin expression were conducted on kidneys fixed in formalin. The in vitro assay was performed by turbidimetry. Phytochemical analyses of the extract were accomplished by HPLC-UV-DAD, and several compounds were isolated. C. langsdorffii leaf extract was able to avoid stone formation. The number of deposits was 50.30 ± 31.29 at the higher extract dose, compared to the value of 179.5 ± 45.96 achieved with the EG control. Significantly lower oxalate levels and OPN expression and increased citrate levels were observed after extract administration. In the in vitro assay, the extract diluted the formed crystals. Phytochemical analyses showed that the extract is rich in phenolic compounds that are capable of preventing stone formation. Thus, on the basis of our results, we suggest that the C. langsdorffii leaf extract has potential application in the prevention of kidney stone formation.

Investigation of the Effect of Dandelion Plant (Taraxacum Officinale) on Calcium Oxalate Monohydrate Crystal Growth

“…(De Oliveira et al, 2013;Akyol et al, 2016). Karahindiba bitkisinde bulunan asidik gruplarının (fenolik asitlerin) kristalin aktif büyüyen yüzeylerine adsorpsiyonunun ve de -COOH grupları ile Ca 2+ kristalitlerinin etkileşiminin kalsiyum oksalat çökmesini engellediği düşünülmektedir.…”

“…Ayrıca, torexacin, retinol, levulin, inulin gibi bileşikler içerir (Anonim, 2016). Karahindiba kristalizasyon engelleyici olarak etkili olduğu düşünülen fenolik asit bakımından da zengin bir bitkidir (Oliveira et al, 2013;Ivanov, 2014).…”

Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

“…kidney stone formation, in vaginal cream on the reproductive performance of female rats among others [6,[8][9][10][11][12]. Nevertheless, there is no data concerning its effect on human immune cells.…”

Avaliação farmacocinética do extrato bruto e de metabólitos secundários das folhas de Copaifera langsdorffii Desf.

“…Sendo assim, animais tratados com extrato hidroalcoólico de C. langsdorffii demonstraram que a média da massa total dos cálculos foi discretamente menor em comparação ao grupo controle (BRANCALION et al, 2012). Em outro estudo foi avaliado o potencial profilático do extrato no tratamento de pedras renais em ratos induzidos a nefrolitíase por etilenoglicol (OLIVEIRA et al, 2013).…”

“…De acordo com Schimidt; Gonzalez & Derendorf (2010) é possível prever a atividade farmacológica de uma substância, quando a mesma é encontrada na forma livre no seu sítio de ação. Contextualizando esta afirmação, pode-se inferir que a presença de metabólitos secundário de C. langsdorffii nos rins dos animais, pode estar relacionada com a cura/prevenção (BRANCALION et al, 2012;OLIVEIRA et al, 2013) da urolitíase.…”