In vitro determination of the uterine stimulatory effect of the aqueous leaf extract of Ficus exasperata

Bafor, Enitome E.; Omogbai, Eric K. I.; Ozolua, Raymond I.

doi:10.1016/j.jep.2009.10.006

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…[91] Considering this observation, Bafor and co-workers extensively studied the effects of various concentrations of aqueous leaf extract of Ficus exasperata on uterine contractions in vitro. [17,92,93] Aqueous leaf extract of F. exasperata at the dose of 1.0 × 10 -2 mg mL -1 inhibited oxytocin-induced uterine contractions without significantly affecting acetylcholine or ergometrine-induced uterine contractions in isolated rat uterus. The results also indicated that the extract had no significant effect on the amplitude and frequency of spontaneous contractions.…”

Section: Uterotonicmentioning

confidence: 94%

“…However, diphenhydramine and phentolamine significantly inhibited the extract suggesting stimulation of uterine contractility by the extract might be due to the activation of histamine H1-and/or α-adrenergic receptors, interference with calcium channels and/or stimulation of prostaglandin synthesis in utero. [93] Antimicrobial The ethanol extract of F. exasperata leaves showed moderate anti-bacterial activity against Escherichia coli and Staphylococcus albus with minimum inhibitory concentrations (MIC) of 300 and 700mg mL -1 , respectively, while that of S. albusis 700mg mL -1 . However, the crudeplant extract in combination with the protein synthesis inhibitors exhibited significant antibacterial activity.…”

Section: Uterotonicmentioning

confidence: 99%

See 1 more Smart Citation

Traditional uses and pharmacological potential of Ficus exasperata vahl

Ahmed¹,

Ahmed²,

Abedin³

et al. 2012

Syst Rev Pharm

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Section: Uterotonicmentioning

confidence: 94%

Section: Uterotonicmentioning

confidence: 99%

Traditional uses and pharmacological potential of Ficus exasperata vahl

Ahmed¹,

Ahmed²,

Abedin³

et al. 2012

Syst Rev Pharm

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“…They have found that metabolites generated from this extract possess both tocolytic and uterotonic activities. Earlier, in-vitro contraction studies using isolated rat’s uteri revealed both stimulatory [17] and inhibitory [18] effects of the unpurified extract of Ficus exasperata. Further to this, Watcho et al, [1] demonstrated that fruit extract of Ficus asperifolia stimulates in-vitro contraction of the uteri isolated from oestrogenized rats.…”

Section: Introductionmentioning

confidence: 99%

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Salleh

Ahmad

2013

BMC Complement Altern Med

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BackgroundFicus deltoidea, is a perennial herb that is used to assist labor, firm the uterus post-delivery and to prevent postpartum bleeding. In view of its claimed uterotonic action, the mechanisms underlying plant’s effect on uterine contraction were investigated.MethodsAdult female SD rats were injected with 2 mg/kg 17β-oestradiol (E2) to synchronize their oestrous cycle. A day after injection, uteri were removed for in-vitro contraction studies. The dose dependent effect of Ficus deltoidea aqeous extract (FDA) on the tension produced by the isolated rat’s uteri was determined. The effects of atropine (2×10-8 M), atosiban (0.5 IU), THG113.31 (10 μM), oxodipine (0.25 mM), EDTA (1 mM), 2-amino-ethoxy-diphenylborate (2-APB) (40 mM) and thapsigargin (1 mM) on the maximum force of contraction (Emax) achieved following 2 mg/ml FDA administration were also investigated.ResultsFDA induced in-vitro contraction of the isolated rat’s uteri in a dose-dependent manner. Administration of atropine, atosiban and THG113.31 reduced the Emax with atosiban having the greatest effect. The Emax was also reduced following oxodipine and EDTA administration. There was no significant change observed following 2-APB administration. Thapsigargin, however, augmented Emax.ConclusionsFDA-induced contraction of the isolated rat’s uteri is mediated via multiple uterotonin receptors (muscarinic, oxytocin and prostaglandin F2α) and was dependent on the extracellular Ca2+. Contraction, however, was not dependent on the Ca2+ release from the internal stores. This in-vitro study provides the first scientific evidence on the claimed effect of Ficus Deltoidea on uterine contraction.

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“…Twenty-four hours prior to the day of experiments, each mouse was administered 1.0 mg/kg diethylstilbestrol orally by using a feeding syringe (Bafor, Omogbai & Ozolua 2010). This dose and route of administration had been previously determined in our laboratory to effectively induce oestrous.…”

Section: Contractility Studies Uterine Tissue Preparationmentioning

confidence: 99%

<i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity

Bafor

Omokaro

Uwumarongie³

et al. 2017

JOMPED

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Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects.Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.

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In vitro determination of the uterine stimulatory effect of the aqueous leaf extract of Ficus exasperata

Cited by 18 publications

References 24 publications

Traditional uses and pharmacological potential of Ficus exasperata vahl

Traditional uses and pharmacological potential of Ficus exasperata vahl

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

<i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity

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