In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Abstract

BackgroundFicus deltoidea, is a perennial herb that is used to assist labor, firm the uterus post-delivery and to prevent postpartum bleeding. In view of its claimed uterotonic action, the mechanisms underlying plant’s effect on uterine contraction were investigated.MethodsAdult female SD rats were injected with 2 mg/kg 17β-oestradiol (E2) to synchronize their oestrous cycle. A day after injection, uteri were removed for in-vitro contraction studies. The dose dependent effect of Ficus deltoidea aqeous extract (FDA) on the tension produced by the isolated rat’s uteri was determined. The effects of atropine (2×10-8 M), atosiban (0.5 IU), THG113.31 (10 μM), oxodipine (0.25 mM), EDTA (1 mM), 2-amino-ethoxy-diphenylborate (2-APB) (40 mM) and thapsigargin (1 mM) on the maximum force of contraction (Emax) achieved following 2 mg/ml FDA administration were also investigated.ResultsFDA induced in-vitro contraction of the isolated rat’s uteri in a dose-dependent manner. Administration of atropine, atosiban and THG113.31 reduced the Emax with atosiban having the greatest effect. The Emax was also reduced following oxodipine and EDTA administration. There was no significant change observed following 2-APB administration. Thapsigargin, however, augmented Emax.ConclusionsFDA-induced contraction of the isolated rat’s uteri is mediated via multiple uterotonin receptors (muscarinic, oxytocin and prostaglandin F2α) and was dependent on the extracellular Ca2+. Contraction, however, was not dependent on the Ca2+ release from the internal stores. This in-vitro study provides the first scientific evidence on the claimed effect of Ficus Deltoidea on uterine contraction.

Ficus deltoidea promotes bone formation in streptozotocin-induced diabetic rats

“…The decoction of F. deltoidea has traditionally been used in postpartum care specifically to improve uterine strength, regain energy, and prevent postpartum bleeding (Sulaiman et al. 2008 ; Salleh and Ahmad 2013 ). It is also served as a health tonic or taken as herbal tea to relieve headache, fever, and toothache (Bunawan et al.…”

Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats

“…This medicinal plant is locally known as Mas Cotek due to the tiny golden spots on the leaves. Extensive pharmacological studies have validated the traditional use of Ficus deltoidea , particularly for maintenance and fertility of the female reproductive system [ 17 ]. Apart from its beneficial effects on female reproductive system, this medicinal plant has also been reported to have antidiabetic [ 18 ], anti-inflammatory and anti-nociceptive [ 19 ], anti-melanogenic and anti-photoaging [ 20 ], anti-bacterial [ 21 ], wound healing [ 22 ], anti-cancer and cytotoxicity properties [ 23 ].…”

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

“…Similar studies using rat [12,25] and mouse [26] uterine strip have led to conclude that bioactive compounds found in L. acida are responsible for the contractile effects. In the current study, the maximal concentration (2.18 mg/ml) which gives the maximal effect is highest than the maximal concentration (1.6 mg/ml and 2 mg/ml) used respectively by Watcho [19] and salleh and Ahmad [27].…”