Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

Abstract

Background: Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to solve di cult childbirth. This study aimed to evaluate the in vitro uterotonic effects of aqueous and methanolic extracts of L. acida in female Wistar rats. Uterine strips isolated from female rats pretreated (48h) with oestradiol (5μg) were mounted in a singleorgan bath containing a well aerated and thermostated De Jalon solution (37°C). The effects of L. acida extracts were recorded in a non-cumulative manner after application. The effect of the methanolic extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a speci c type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist) and 2-Aminoethoxydiphenyl borate (2-ADB, a speci c antagonist of inositol 1,4,5triphosphate receptors type 1), and in calcium-free medium containing EGTA.Results: L. acidainduced uterine contraction in a concentration-dependent manner with the methanolic extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2 μmol/l), atropine (1 μmol/l), nifedipine (5 μmol), 2-APB (100 μmol), and calcium free medium containing EGTA (2 mmol) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium free medium containing EGTA.Conclusions: These results suggest that L. acida possesses an uterotonic effect mediated through oxytocin receptors with mobilization of extracellular calcium.

Comparative evaluation of the potential anti-spasmodic activity of Piper longum, Piper nigrum, Terminalia bellerica, Terminalia chebula, and Zingiber officinale in experimental animals

“…The composition of Tyrode solution in mM is NaCl 137, KCl 2.7, NaHCO 3 11.9, NaH 2 PO 4 0.4, MgCl 2 0.1–1.0, CaCl 2 1.8, and glucose 11.1. De Jalon was used in the rat uterus preparation and prepared as per the procedure described in the literature ( Ngadjui et al, 2021 ). The composition of De Jalon solution in mM is NaCl 154, KCl 5.6, NaHCO 3 6.0, CaCl 2 0.55, and glucose 2.78.…”

Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of <i>Anastatica hierochuntica</i> L. (Brassicaceae) on the Isolated Uterus of Mice

“…A mouse uterus was prepared according to the procedure described by [8]. After cervical dislocation, the mice uteri were collected and promptly removed.…”

“…Therefore, scientific research for new safe and effective uterotonic and tocolytic agents arouses considerable interest in public health [7]. In the literature, the contractile effect of certain plants commonly used to induce labor and facilitate delivery has been demonstrated, among others, Matricaria chamomilla (Asteraceae) [5], Lannea acida (Anacardiaceae) [8], Ananas comosus (Bromeliaceae) [9], Jussiaea repens (Onagraceae) [10].…”

Determination of potential sources of drug development for menstrual disorders: A qualitative analysis of published literature of in-vitro rat uterus experimental studies

“…The duplex uterus is common in some animals and will accommodate multiple embryos (a litter). 15,16 For in vitro pharmacological experiments on rat uterus, the rat is sacrificed and sections of uterus are oriented to measure changes in contraction of longitudinal or circular muscle layers, and mounted in 14 ml organ baths in physiological salt solution with aeration of 95% 0 2 and 5% C0 2 at 37 • C. The De Jalon's solution is used in the preparation of rat uterus for experiment. This is a physiological solution that is used to maintain the viability of tissues.…”