<i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity

Abstract

Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects.Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p &lt; 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.

Toxic action of substances from male fern Dryopteris filix-mas (L.) Schott (1834) on free-living soil nematode Caenorhabditis elegans Maupas (1900)

“…After third stage the rough filtration through lavsan filters was carried out followed by precipitation of suspended particles for 15 min at 4500 rpm (centrifuge VS-1536E) and final filtration through SF paper (slow filtration). Obtained extract was separated into two equal volume parts and dried under reduced pressure in vacuum rotary evaporator IR-1LT (15 mmHg, 25℃) to full remove of extragent [13,14].…”

“…The content of kaempferol-3-O-glucoside was determined by HPLC method using standard [15]. The amount of filixic acid was proved by combine method with use of HPLC, NMR С 13 and H 1 [18]. Phytochemical characteristics of obtained extracts are shown in Table 1.…”

Antioxidant Activities of the Leaf Extract and Fractions of Dryopteris filix-mas (L.) Schott could be Attributed to The Abundance of Polyphenol Compounds

“…Previous studies have found that it possesses antihelmintic activity (Urban et al, 2014), antimicrobial activity (Mandal andMondal, 2014), antidiarrheal (Uwumarongi et al, 2016), uterine relaxant (Bafor et al, 2017) and anti-inflammatory (Erhirhie et al, 2019) activities. A preliminary study by Sekender et al, (2012) revealed that it possesses anti-oxidant activity.…”

“…Its leaves are bipinnated and consist of 20-35 pinnae on each side of the rachis (Fig 1). Its stalks are covered with orange-brown scales (Bafor et al, 2017). The leaf decoction is ethno medicinally used in rheumatoid arthritis, wounds, bleeding disorders, ulcers, worm infestation and malaria (Tagarelli et al, 2010;Erhirhie et al, 2019).…”

Çocuk Hastaliklarinda Geleneksel Olarak Kullanilan Bazi Bi̇tki̇ler

“…Approximately 80% of the global population, particularly those of rural settlements, depend on medicinal plants in various forms as medicines for the maintenance of their health [11]. In the Western countries with highly developed health care systems, phytotherapy is considered as a part of complementary and Alternative Medicine (CAM) and it has significantly increased in recent years [12]. Globally, the use of medicinal plants for childcare and general well-being has long been recognized.…”