In vitro degradation behavior of chitosan based hybrid microparticles

Abstract

The degradation properties of the MPs is important to the long-term benefits of the use of the chitosan (CS) based hybrid MPs in bone tissue-engineering, because the degradation kinetics could affect a multitude of processes within the cell, such as cell growth, tissue regeneration, and host response. The aim of this study was to investigate the degradation of solid, hybrid CS microparticles (MPs), CS-10% calcium phosphate (CaHPO4, w/w), and CS-10% calcium carbonate (CaCO3, w/w) MPs in phosphate buffered solution (PBS) over a 30-week period. The hybrid MPs were synthesized by emulsification technique, cross-linked with 64% sodium tripolyphosphate (TPP), purified and air dried overnight. Each sample had 30 mg of MPs was placed in a glass vial with 9 ml of PBS added and then the vial was closed to prevent evaporation. Every week 4 ml of the incubated solution was removed for sample measurement and all samples were replaced with an equivalent amount of fresh medium. The samples were maintained at 37°C under continuous shaking. The hybrid MPs were measured for pH and calcium release, every week in triplicate. At 0, 5, 10, 15, 20, 25, and 30 weeks, surface and bulk morphology were analyzed with a scanning electron microscope (SEM). The degradation data suggested that the hybrid MPs were stable at least up to 25 week and maintain the physiologically relevant pH. Therefore, we can use these hybrid MPs to apply in the bone tissue engineering applications since they do not degrade within a short period.

Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery

“…Mechanical measurements showed that addition of HA significantly increased the elastic modulus of CHI-PVA-HA-Hep-GA cryogel (1085 ± 428 kPa) and that the value was significantly higher in comparison to other cryogel-based scaffolds designed for bone regeneration [43][44][45][46][47][48][49]. Degradation experiments confirmed a decreased in vitro degradation rate of HA-containing cryogels, which is in line with other published data [45,50]. A lower degradation rate could be explained by the reduction of Schiff's base groups after NaBH 4 addition, which removes hydrolytically active groups [51].…”

Enhancing the biological activity of chitosan and controlling the degradation by nanoscale interaction with bioglass

“…It is favorable to develop a material that its degradation rate is balanced with the extracellular matrix formation during tissue regeneration. Therefore, it is envisaged that a material supports the transfer of structural and functional roles of host tissue, maintains mechanical strength during tissue regeneration, and finally reabsorbs and metabolized by the body . The degradation rate of chitosan can be adjusted by varying the degree of deacetylation from 9 days to over 85 days .…”

Current wound healing procedures and potential care

“…Chitosan is biocompatible, biodegradable, and non toxic with cell cultures in vitro and in vivo animal experiments [83–87]. In addition, chitosan has been shown to have intrinsic antimicrobacterial properties on bacteria and fungai, and to be haemostatic.…”